ID G5C9Y4_HETGA Unreviewed; 4388 AA.
AC G5C9Y4;
DT 14-DEC-2011, integrated into UniProtKB/TrEMBL.
DT 14-DEC-2011, sequence version 1.
DT 24-JAN-2024, entry version 58.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=GW7_13236 {ECO:0000313|EMBL:EHB18345.1};
OS Heterocephalus glaber (Naked mole rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Bathyergidae;
OC Heterocephalus.
OX NCBI_TaxID=10181 {ECO:0000313|EMBL:EHB18345.1, ECO:0000313|Proteomes:UP000006813};
RN [1] {ECO:0000313|EMBL:EHB18345.1, ECO:0000313|Proteomes:UP000006813}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21993625; DOI=10.1038/nature10533;
RA Kim E.B., Fang X., Fushan A.A., Huang Z., Lobanov A.V., Han L.,
RA Marino S.M., Sun X., Turanov A.A., Yang P., Yim S.H., Zhao X.,
RA Kasaikina M.V., Stoletzki N., Peng C., Polak P., Xiong Z., Kiezun A.,
RA Zhu Y., Chen Y., Kryukov G.V., Zhang Q., Peshkin L., Yang L., Bronson R.T.,
RA Buffenstein R., Wang B., Han C., Li Q., Chen L., Zhao W., Sunyaev S.R.,
RA Park T.J., Zhang G., Wang J., Gladyshev V.N.;
RT "Genome sequencing reveals insights into physiology and longevity of the
RT naked mole rat.";
RL Nature 479:223-227(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the UPL family. TOM1/PTR1 subfamily.
CC {ECO:0000256|ARBA:ARBA00034494}.
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DR EMBL; JH174082; EHB18345.1; -; Genomic_DNA.
DR STRING; 10181.G5C9Y4; -.
DR eggNOG; KOG0939; Eukaryota.
DR InParanoid; G5C9Y4; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000006813; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd00078; HECTc; 1.
DR CDD; cd14288; UBA_HUWE1; 1.
DR Gene3D; 3.30.720.50; -; 1.
DR Gene3D; 6.10.250.1630; -; 1.
DR Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR010309; E3_Ub_ligase_DUF908.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR InterPro; IPR015940; UBA.
DR InterPro; IPR009060; UBA-like_sf.
DR InterPro; IPR041918; UBA_HUWE1.
DR InterPro; IPR004170; WWE-dom.
DR InterPro; IPR037197; WWE_dom_sf.
DR PANTHER; PTHR11254:SF67; E3 UBIQUITIN-PROTEIN LIGASE HUWE1; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF06012; DUF908; 1.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF00627; UBA; 1.
DR Pfam; PF14377; UBM; 3.
DR Pfam; PF02825; WWE; 1.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00165; UBA; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF46934; UBA-like; 1.
DR SUPFAM; SSF117839; WWE domain; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50030; UBA; 1.
DR PROSITE; PS50918; WWE; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000006813};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 1315..1354
FT /note="UBA"
FT /evidence="ECO:0000259|PROSITE:PS50030"
FT DOMAIN 1602..1679
FT /note="WWE"
FT /evidence="ECO:0000259|PROSITE:PS50918"
FT DOMAIN 4049..4388
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 705..758
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 978..1006
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1290..1319
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1395..1414
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1689..1717
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2016..2065
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2262..2343
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2355..2480
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2706..2982
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3001..3024
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3049..3071
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3256..3279
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3366..3386
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3417..3438
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3484..3520
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3545..3576
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3747..3768
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3791..3858
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3905..3942
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 737..756
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1290..1309
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1698..1717
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2016..2035
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2036..2060
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2262..2295
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2384..2402
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2406..2471
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2706..2730
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2731..2781
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2827..2899
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2917..2946
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3002..3024
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3053..3071
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3256..3272
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3545..3565
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3793..3809
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3823..3840
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3905..3922
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 4355
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 4388 AA; 483198 MW; 696EC4D67C715F9B CRC64;
MKVDRTKLKK TPTEAPADCR ALIDKLKVCN DEQLLLELQQ IKTWNIGKCE LYHWVDLLDR
FDGILADAGQ TVENMSWMLV CDRPEREQLK MLLLAVLNFT ALLIEYSFSR HLYSSIEHLT
TLLASSDMQV VLAVLNLLYV FSKRSNYITR LGSDKRTPLL TRLQHLAESW GGKENGFGLA
ECCRDLHMMK YPPSATTLHF EFYADPGAEV KIEKKTTSNT LHYIHIEQLD KISESPSEIM
ESLTKMYSIP KDKQMLLFTH IRLAHGFSNH RKRLQAVQAR LHAISILVYS NALQESANSI
LYNGLIEELV DVLQITDKQL MEIKAASLRT LTSIVHLERT PKLSSIIDCT GTASYHGFLP
VLVRNCIQAM IDPSMDPYPH QFATALFSFL YHLASYDAGG EALVSCGMME ALLKVIKFLG
DEQDQITFVT RAVRVVDLIT NLDMAAFQSH SGLSIFIYRL EHEVDLCRKE CPFVIKPKIQ
RPSNTQEGEE METDMDGVQC IPQRAALLKS MLNFLKKAIQ DPAFSDGIRH VMDGSLPTSL
KHIISNAEYY GPSLFLLATE VVTVFVFQEP SLLSSLQDNG LTDVMLHALL IKDVPATREV
LGSLPNVFSA LCLNARGLQS FVQCQPFERL FKVLLSPDYL PAMRRRRSSD PLGDTASNLG
SAVDELMRHQ PTLKTDATTA IIKLLEEICN LGRDPKYICQ KPSIQKADGT AAAPPPRSNH
AAEEASSEDE EEEEVQAMQS FNSAQQSETE PNQQVVGTEE RIPIPLMDYI LNVMKFVESI
LSNNTTDDHC QEFVNQKGLL PLVTILGLPN LPIDFPTSAA CQAVAGVCKS ILTLSHEPKV
LQEGLLQLDS ILSSLEPLHR PIESPGGSVL LRELACAGNV ADATLSAQAT PLLHALTAAH
AYIMMFVHTC RVGQSEIRSI SVNQWGSQLG LSVLSKLSQL YCSLVWESTV LLSLCTPNSL
PSGCEFGQAD MQKLVPKDEK AGTTQSGKRS DGEQDGTAGG MDASTQGLLE GIGLDGDTLA
PMETDEPASE AKAKSKITPA MAARIKQIKP LLSASSRLGR ALAELFGLLV KLCVGSPVRQ
RRSHHAASTT TAPTPAARST ASALTKLLTK GLSWQPPPYT PTPRFRLTFF ICSVGFTSPM
LFDERKYPYH LMLQKFLCSG GHNALFETFN WALSMGGKVP VSEGLEHSDL PDGTGEFLDA
WLMLVEKMVN PTTVLESPHS LPAKLPGGVQ NFPQFSALRF LVVTQKVPFT CIKNLWNRKP
LKVYGGRMAE SMLAILCHIL RGEPVIRERL SKEKEGSRGE EDAGQEEGGS RREPQVNQQQ
LQQLIDMGFT REHAMEALLN TSTMEQATEY LLTHPPPIMG GVVRDLSMSE EDQMMRAIAM
SLGQDIPMDQ RAESPEEVAC RKEEEERKAR EKQEEEEAKC LEKFQDADPL EQDELHTFTD
TMLPGCFHLL DELPDTVYRV CDLIMTAIKR NGADYRDMIL KQVVNQVWEA ADVLIKAALP
LTTSDTKTVS EWISQMATLP QASNLATRIL LLTLLFEELK LPCAWVVESS GILNVLIKLL
EVVQPCLQAA KEQKEVQTPK WITPVLLLID FYEKTAISSK RRAQMTKYLQ SNSNNWRWFD
DRSGRWCSYS ASNNSTIDSA WKSGETSVRF TAGRRRYTVQ FTTMVQVNEE TGNRRPVMLT
LLRVPRLNKN SKNNNGQELE KTLEESKEMD IKRKENKGSD TSLTLEITNT EKEVSLEETK
IGEILIQGLT EDMVTVLIRA CVSMLGVPVD PDTLHATLRL CLRLTRDHKY AMMFAELKST
RMILSLTQSS GFNGFTPLVT LLLRHIIEDP CTLRHTMEKV VRSAATSGAG STTSGVVSGS
LGSREINYIL RVLGPAACRN PDIFTEVANC CIRIALPAPR GSGTASDDEF ENLRIKGPNA
VQLVKTTPLK PSSLPVIPDT IKEVIYDMLN ALAAYHAPEE ADKSDPKPGG MTQEVGQLLQ
DMGDDVYQQY RSLTRHSSDF DTQSSFSLNS QVFAADGASG ETSTSGTSQG EGASTPEESR
DGKKDKEGDR ASEENKQKGK GSKPLMPTST ILRLLAELVR SYVGIATLIA NYSYTVGQSE
LIKEDCSVLA FVLDHLLPHT QNAEDKDTPA LARLFLASLA AAGSGTDAQV ALVNEVKAAL
GRALAMAEST EKHARLQAVM CIISTIMESC PSTSSFYSSA TAKTQHNGMN NIIRLFLKKG
LVNDLARVPH SLDLSSPNMA NTVNAALKPL ETLSRIVNQP SSLFGSKSAS SKSKSEQDAQ
GASQDSNSNQ QDPGEPGEAE VQEEDHDVTQ TEVADGDIMD GEAETDSVVI AGQPEVLSSQ
EMQVENELED LIDELLERDG GSGNSTIIVS RSGEDESQED VLMDEAPSNL SQASTLQANR
EDSMNILDPE DEEEHTQEED SSGSNEDEDD SQDEEEEEEE DEEDDQEDDE GEEGDEDDDD
DGSEMELDED YPDMNASPLV RFERFDREDD LIIEFDNMFS SATDIPPSPG NIPTTHPLMV
RHADHSSLTL GSGSSTTRLT QGIGRSQRTL RQLTANTGHT IHVHYPGNRQ PNPPLILQRL
LGPSAAADIL QLSSSLPLQS RGRARLLVGN DDVHIIARSD DELLDDFFHD QSTATSQAGK
AKNLNLCSPT GTLSSIPTAL TRWTEECKVL DAESMHDCVS VVKVPIVNHL EFLRDEELEE
RREKRRKQLA EEETKITDKG KEDKENRDQS AQCTASKTDD STEQNLSDGT PMPDSYPTTP
SSTDAATSES KETLGTLQPS QQQPALPPPP ALEEVPQELQ SPAGEGGSSA QLLMPVESEE
LGPTRPSGEA ETTQMELSPA PTITSLSPER AEDSDALTAV SSQLEGSPMD TSSLASCTLE
EAVGDTSAAG NSEQPTAGGS TPGDALPVVA EAQGRADGTG ESTQPTEDSS PPASSESSST
RDSAVAISGA DSRGILEEPL PSTSSEEEDP LAGISLPEGV DPSFLAALPD DIRREVLQNQ
LGIRPPTRSA PSTNSTATTV VGNPGVTEVS PEFLAALPPA IQEEVLAQQR AEQQRRELAQ
NASSDTPMDP VTFIQTLPSD LRRSVLEDME DSVLAVMPPD IAAEAQALRR EQEARQRQLM
HERLFGHSST SALSAILRSP AFTSRLSGNR GVQYTRLAVQ RGGTFQMGGS SSHNRPSGSN
VDTLLRLRGR LLLDHEALSC LLVLLFVDEP KLNTSRLHRV LRNLCYHAQT RHWVIRSLLS
ILQRSSESEL CIETPKLSTS EEKGKKSSKS CGSSSHENRP LDLLHKMESK SSNQLSWLSV
SMDAALGCRT NIFQIQRSGG RKHTEKHASS GSTVHIHPQA APVVCRHVLD TLIQLAKVFP
SHFTQQRTKE TNCESDRERG SKQACSPCSS QTTSSGICTD FWDLLVKLDN MNVSRKGKNS
VKSVPVSTGG EGETSPYSLE ASPLGQLMNM LSHPVIRRSS LLTEKLLRLL SLISIALPEN
KVSEAQANSG SSASSTTAAS STTSTATTTA TATPPAATTP VTSAPALVAA TAISTVAVAA
STTVTTPTTA TTTVSTSTTT KGSKSPAKVG EGGSSGADFK MVSSGLTENQ LQLSVEVLTS
HSCSEEGLED AANVLLQLSR GDPGTRDTVL KLLLNGARHL GYTLCKQIGT LLAELREYNL
EQQRRAQCET LSPDGLPEEQ PQTTKLKGKM QSRFDMAENV VIVASQKRPL GGRELQLPSM
SMLTSKTSTQ KFFLRVLQVI IQLRDDTRRA NKKAKQTGRL GSSGLGSASS IQAAVRQLEA
EADAIIQMVR EGQRARRQQQ AATSESSQSE ASVRREESPM DVDQPSPSAQ DTQSIVASDG
TPQGEKEKEE RPPELPLLSE QLSLDELWDM LGECLKELEE SHDQHAVLVL QPAVEAFFLV
HATERESKPP VRDTRESQLA HIKDEPPPLS PAPLTPATPS SLDPFFSREP SSMHISSSLP
PDTQKFLRFA ETHRTVLNQI LRQSTTHLAD GPFAVLVDYI RVLDFDVKRK YFRQELERLD
EGLRKEDMAV HVRRDHVFED SYRELHRKSP EEMKNRLYIV FEGEEGQDAG GLLREWYMII
SREMFNPMYA LFRTSPGDRV TYTINPSSHC NPNHLSYFKF VGRIVAKAVY DNRLLECYFT
RSFYKHILGK SVRYTDMESE DYHFYQGLVY LLENDVSTLG YDLTFSTEVQ EFGVCEVRDL
KPNGANILVT EENKKEYVHL VCQMRMTGAI RKQLAAFLEG FYEIIPKRLI SIFTEQELEL
LISGLPTIDI DDLKSNTEYH KYQSNSIQVS FCCWFWRALR SFDQADRAKF LQFVTGTSKV
PLQGFAALEG MNGIQKFQIH RDDRSTDRLP SAHTCFNQLD LPAYESFEKL RHMLLLAIQE
CSEGFGLA
//
