ID G5JZQ9_9STRE Unreviewed; 905 AA.
AC G5JZQ9;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN ORFNames=STRIC_0773 {ECO:0000313|EMBL:EHI70994.1};
OS Streptococcus ictaluri 707-05.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=764299 {ECO:0000313|EMBL:EHI70994.1, ECO:0000313|Proteomes:UP000003330};
RN [1] {ECO:0000313|EMBL:EHI70994.1, ECO:0000313|Proteomes:UP000003330}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=707-05 {ECO:0000313|EMBL:EHI70994.1,
RC ECO:0000313|Proteomes:UP000003330};
RX PubMed=24625962; DOI=10.1093/gbe/evu048;
RA Richards V.P., Palmer S.R., Pavinski Bitar P.D., Qin X., Weinstock G.M.,
RA Highlander S.K., Town C.D., Burne R.A., Stanhope M.J.;
RT "Phylogenomics and the dynamic genome evolution of the genus
RT Streptococcus.";
RL Genome Biol. Evol. 6:741-753(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EHI70994.1}.
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DR EMBL; AEUX02000001; EHI70994.1; -; Genomic_DNA.
DR AlphaFoldDB; G5JZQ9; -.
DR STRING; 764299.STRIC_0773; -.
DR eggNOG; COG1523; Bacteria.
DR Proteomes; UP000003330; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 2.60.40.1220; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR014755; Cu-Rt/internalin_Ig-like.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR019931; LPXTG_anchor.
DR InterPro; IPR011838; Pullulan_Gpos.
DR InterPro; IPR040806; SpuA_C.
DR NCBIfam; TIGR01167; LPXTG_anchor; 1.
DR NCBIfam; TIGR02102; pullulan_Gpos; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR Pfam; PF00746; Gram_pos_anchor; 1.
DR Pfam; PF18033; SpuA_C; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE 4: Predicted;
KW Cell wall {ECO:0000256|ARBA:ARBA00022512};
KW Peptidoglycan-anchor {ECO:0000256|ARBA:ARBA00023088};
KW Reference proteome {ECO:0000313|Proteomes:UP000003330};
KW Secreted {ECO:0000256|ARBA:ARBA00022512};
KW Signal {ECO:0000256|ARBA:ARBA00022729}.
FT DOMAIN 871..905
FT /note="Gram-positive cocci surface proteins LPxTG"
FT /evidence="ECO:0000259|PROSITE:PS50847"
SQ SEQUENCE 905 AA; 100673 MW; 0AD86A06AC070B55 CRC64;
MILDKSKSGD AIKVQPKDYL FKDLVNHTQI FVKGTDPKVY NNPYYIDQVS LKGAQQTELD
ELRASFTTLD GLDKDDILKA IKVMDKDGKE VAIDDLSFDK ENPVVILKGD FKVAGSDYTV
TFGDLSQVAR QSWQLKDKLY TYEGELGANL FKDGSVDLAL WSPSADSVKV VIYDKDDQTK
VLGQVDLTKS EKGVWTSHLS ADSIRGISDY TGYYYLYEIT RGQKKVMVLD PYAKSLAAWD
SKTVTDEIKT AKAAFVNSSS LGPKDLGFAK IENFSKREDA IIYGAHVRDF TSDQALDGKL
SSPFGTFSAF VERLDYLKDL GVTHVQLLPV LSYFYVNELD KARSTAYTSS DNNYNWGYDP
QSYFALSGMY SENPKDPALR ISELKELVNE IHKRGMGVIL DVVYNHTART YLFEDLEPHY
YHFMNADGTA RESFGGGRLG TTHAMSRRVL VDSITYLTNE FKVDGFRFDM MGDHDAQAID
QAFQAAKAIN PNTIMIGEGW RTYQGDEGHK EQAADQEMKA TDTVGVFSDD IRNILKTGFP
NEGTPAFITG GAKDIESLFR SIKAQPANFE ADDPGDVVQY IAAHDNLTLH DVIAKSLNLD
PKSAQEEIHR RIRLGNTLIL TSQGTAFLHS GQEYGRSKQL LNKDYKSKVT DDKVPNKATL
IDAVKEYPYF IHDSYDSSDA VNHFDWTKAT DKEFNPISTN TQAYTAGLIA LRRSTDAFCK
ASKAEVEASV HLIGQIGQDG IEKEDKLIAY QTRDSNGDLY AIFVNADSQS RRLRLNDKYR
FLLKGQVIVD ADRAGTSELS NPKGVSFTKD AINLDALTAI VIKVEAPDVD NIEQSHPSKE
MTEVMPLVTQ SAKNSETISR QTQSHAIKET LPKTGTQDSK GLVAMGLLAL SAAFRLVTFK
TSSKN
//
