ID G5ZXY6_9PROT Unreviewed; 1061 AA.
AC G5ZXY6;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 24-JAN-2024, entry version 47.
DE RecName: Full=Error-prone DNA polymerase {ECO:0000256|HAMAP-Rule:MF_01902};
DE EC=2.7.7.7 {ECO:0000256|HAMAP-Rule:MF_01902};
GN Name=dnaE2 {ECO:0000256|HAMAP-Rule:MF_01902};
GN ORFNames=HIMB100_00008870 {ECO:0000313|EMBL:EHI49317.1};
OS SAR116 cluster alpha proteobacterium HIMB100.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; SAR116 cluster.
OX NCBI_TaxID=909943 {ECO:0000313|EMBL:EHI49317.1, ECO:0000313|Proteomes:UP000004261};
RN [1] {ECO:0000313|EMBL:EHI49317.1, ECO:0000313|Proteomes:UP000004261}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=strain HIMB100 {ECO:0000313|Proteomes:UP000004261};
RX PubMed=22675578; DOI=10.4056/sigs.1854551;
RA Grote J., Bayindirli C., Bergauer K., Carpintero de Moraes P., Chen H.,
RA D'Ambrosio L., Edwards B., Fernandez-Gomez B., Hamisi M., Logares R.,
RA Nguyen D., Rii Y.M., Saeck E., Schutte C., Widner B., Church M.J.,
RA Steward G.F., Karl D.M., Delong E.F., Eppley J.M., Schuster S.C.,
RA Kyrpides N.C., Rappe M.S.;
RT "Draft genome sequence of strain HIMB100, a cultured representative of the
RT SAR116 clade of marine Alphaproteobacteria.";
RL Stand. Genomic Sci. 5:269-278(2011).
CC -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC translesion synthesis (TLS). It is not the major replicative DNA
CC polymerase. {ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632, ECO:0000256|HAMAP-
CC Rule:MF_01902};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC subfamily. {ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EHI49317.1}.
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DR EMBL; AFXB01000006; EHI49317.1; -; Genomic_DNA.
DR AlphaFoldDB; G5ZXY6; -.
DR STRING; 909943.HIMB100_00008870; -.
DR PATRIC; fig|909943.3.peg.904; -.
DR eggNOG; COG0587; Bacteria.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000004261; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd07434; PHP_PolIIIA_DnaE2; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR HAMAP; MF_01902; DNApol_error_prone; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR023073; DnaE2.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004013; PHP_dom.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF4; ERROR-PRONE DNA POLYMERASE; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_01902};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|HAMAP-Rule:MF_01902};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_01902}; Reference proteome {ECO:0000313|Proteomes:UP000004261};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_01902}.
FT DOMAIN 15..137
FT /note="PHP"
FT /evidence="ECO:0000259|Pfam:PF02811"
FT DOMAIN 289..542
FT /note="Bacterial DNA polymerase III alpha subunit NTPase"
FT /evidence="ECO:0000259|Pfam:PF07733"
FT DOMAIN 545..711
FT /note="DNA polymerase III alpha subunit finger"
FT /evidence="ECO:0000259|Pfam:PF17657"
FT DOMAIN 784..872
FT /note="DNA polymerase helix-hairpin-helix motif"
FT /evidence="ECO:0000259|Pfam:PF14579"
SQ SEQUENCE 1061 AA; 118465 MW; 7D8EFCC958FC8A45 CRC64;
MSGRIAYSPK AVLGCQTNFS FLSGASHPEE LISAADQLGW QAVGIADKFS CGGLVRAHMT
IKDLDRCSSV NKLICGVSVT LHDGGDILCY ARSLDGYHSL CQWLSAAMMR SGHDSRVLPD
MHASDLGRLS SDVIVITLPP VLANTDYREQ ARTIALFAPG DVYVGGYLMR DGCDEERLYR
LSYEAAADDL GFVALGHVLY HRPERRPLAD VLSCIRHKTS LPEAGWLLSR NAERHLLAPD
EMARRWQGYE DALRTADIIA ESCQFSLDDL RYDYPAHEGR SGRSAMDELT WQTHEGSKKR
YPDGVPDKVK AYLDKELALI RKLDYAPFFL TVFDIVRFAR SRNILCQGRG SAANSAVCYC
LFITSVDPAR SEPLFERFVS EARNEPPDID VDFEHNRREE VIQYIYHRYG RHRAGLASSV
ITYKGRSAFV EVAKAFGLSR DVQRALSGQV WGRKNNGLNS ESLRAVGLDV QDPTLQMVVK
LARQIQGFPR HLSQHVGGFV IVQDRLDKLC VVRPAAMQGR SIIEWDKDDL DALGLLKIDV
LALGMLSCLA GAFALMRQYY HTSLTLATIP PEDKMTYDML CRGESVGVFQ VESRAQMAML
PRLQPRCFHD LIVQVAIVRP GPIQGDMVHP YLRRRAGLEH IDYPSDELRS VLDRTLGVPL
FQEQAMKIAI VGAGFSGAEA DKLRRAMATF RKNGDIHKFF DKMVNGMIER GYDAEFAQRC
FKQIEGFGTY GFPESHAASF ALLVYASAWL KCHYPEVFTC ALLNAQPMGF YSPAQLISDA
QRSGVEVRPV DINHSAWESI LEPTPQARHA LRLGLRLVKG LNAAEAGRIT ACRGEGYQSV
EEIIYRSDVS AKTIELIAAA DGLRSLGLDA RQGFWQARKQ GRLKMNKLPL FAHADSQGRT
TQDSDNSHEH LPARPFGEQI AQDYASTGLS LKGHPVDSLK LWFEKDRWQS CSIIDEMPNG
RRLRLIGLVT MRQRPGTAKG TVFVTLEDSL LSVNVIVWPH IVERDRTALL GAHLMGVYGR
LQRQGPITHV IADAVHNCDS YLGFLQTATQ KSPHLKSRDF H
//
