ID G7H7M8_9ACTN Unreviewed; 171 AA.
AC G7H7M8;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 24-JAN-2024, entry version 33.
DE RecName: Full=3'-5' exoribonuclease {ECO:0000256|HAMAP-Rule:MF_00977};
DE EC=3.1.13.- {ECO:0000256|HAMAP-Rule:MF_00977};
GN ORFNames=GOARA_089_00350 {ECO:0000313|EMBL:GAB11853.1};
OS Gordonia araii NBRC 100433.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Gordoniaceae;
OC Gordonia.
OX NCBI_TaxID=1073574 {ECO:0000313|EMBL:GAB11853.1, ECO:0000313|Proteomes:UP000035088};
RN [1] {ECO:0000313|EMBL:GAB11853.1, ECO:0000313|Proteomes:UP000035088}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 100433 {ECO:0000313|EMBL:GAB11853.1,
RC ECO:0000313|Proteomes:UP000035088};
RA Yoshida Y., Hosoyama A., Tsuchikane K., Katsumata H., Yamazaki S.,
RA Fujita N.;
RT "Whole genome shotgun sequence of Gordonia araii NBRC 100433.";
RL Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Exonuclease that cleaves single-stranded 3' overhangs of
CC double-stranded RNA. {ECO:0000256|HAMAP-Rule:MF_00977}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00977};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|HAMAP-Rule:MF_00977};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|HAMAP-Rule:MF_00977}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAB11853.1}.
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DR EMBL; BAEE01000089; GAB11853.1; -; Genomic_DNA.
DR RefSeq; WP_007323927.1; NZ_JABELY010000013.1.
DR AlphaFoldDB; G7H7M8; -.
DR STRING; 1073574.GOARA_089_00350; -.
DR OrthoDB; 4640719at2; -.
DR Proteomes; UP000035088; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0004532; F:RNA exonuclease activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR HAMAP; MF_00977; 3_5_Exoribonuc_actinobact; 1.
DR InterPro; IPR030853; 3_5_Exoribonuc_actinobac.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR033390; Rv2179c-like.
DR NCBIfam; NF033638; RNase_AS; 1.
DR Pfam; PF16473; Rv2179c-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 3: Inferred from homology;
KW Exonuclease {ECO:0000256|HAMAP-Rule:MF_00977};
KW Hydrolase {ECO:0000256|HAMAP-Rule:MF_00977};
KW Magnesium {ECO:0000256|HAMAP-Rule:MF_00977};
KW Metal-binding {ECO:0000256|HAMAP-Rule:MF_00977};
KW Nuclease {ECO:0000256|HAMAP-Rule:MF_00977}.
FT DOMAIN 2..155
FT /note="3'-5' exoribonuclease Rv2179c-like"
FT /evidence="ECO:0000259|Pfam:PF16473"
FT REGION 6..9
FT /note="RNA binding"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00977"
FT BINDING 6
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00977"
SQ SEQUENCE 171 AA; 19197 MW; E252D29CE9533932 CRC64;
MRFFYDTEFI EDGRTIDLVS IGVAAADGRE YYAVSTEFDP ERAGDWVRAN VLPKLPSPAS
KAWRSRRQIR DELLEFLTAG GEPVELWAWT GAYDHVALCQ LWGPMTALPR EIPRFTRELR
QVWEDAGRPS LPSLPDDAHD ALADARHNLA KWNRIVGAPD ADPGARGGPL R
//
