ID G7ID01_MEDTR Unreviewed; 1340 AA.
AC G7ID01; A0A0C3UTD7;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2014, sequence version 2.
DT 27-MAR-2024, entry version 70.
DE RecName: Full=Eukaryotic translation initiation factor 5B {ECO:0000256|ARBA:ARBA00013824};
DE AltName: Full=Translation initiation factor IF-2 {ECO:0000256|ARBA:ARBA00032478};
GN Name=11422436 {ECO:0000313|EnsemblPlants:AES62375};
GN OrderedLocusNames=MTR_1g099000 {ECO:0000313|EMBL:AES62375.2};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880 {ECO:0000313|EMBL:AES62375.2, ECO:0000313|Proteomes:UP000002051};
RN [1] {ECO:0000313|EMBL:AES62375.2, ECO:0000313|Proteomes:UP000002051}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A17 {ECO:0000313|EMBL:AES62375.2}, and cv. Jemalong A17
RC {ECO:0000313|EnsemblPlants:AES62375,
RC ECO:0000313|Proteomes:UP000002051};
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E., Geurts R., Cannon S.B., Udvardi M.K.,
RA Benedito V.A., Mayer K.F., Gouzy J., Schoof H., Van de Peer Y., Proost S.,
RA Cook D.R., Meyers B.C., Spannagl M., Cheung F., De Mita S.,
RA Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K., Murray J.D.,
RA Naoumkina M.A., Rosen B., Silverstein K.A., Tang H., Rombauts S.,
RA Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M., Bellec A., Berger A.,
RA Berges H., Bidwell S., Bisseling T., Choisne N., Couloux A., Denny R.,
RA Deshpande S., Dai X., Doyle J.J., Dudez A.M., Farmer A.D., Fouteau S.,
RA Franken C., Gibelin C., Gish J., Goldstein S., Gonzalez A.J., Green P.J.,
RA Hallab A., Hartog M., Hua A., Humphray S.J., Jeong D.H., Jing Y.,
RA Jocker A., Kenton S.M., Kim D.J., Klee K., Lai H., Lang C., Lin S.,
RA Macmil S.L., Magdelenat G., Matthews L., McCorrison J., Monaghan E.L.,
RA Mun J.H., Najar F.Z., Nicholson C., Noirot C., O'Bleness M., Paule C.R.,
RA Poulain J., Prion F., Qin B., Qu C., Retzel E.F., Riddle C., Sallet E.,
RA Samain S., Samson N., Sanders I., Saurat O., Scarpelli C., Schiex T.,
RA Segurens B., Severin A.J., Sherrier D.J., Shi R., Sims S., Singer S.R.,
RA Sinharoy S., Sterck L., Viollet A., Wang B.B., Wang K., Wang M., Wang X.,
RA Warfsmann J., Weissenbach J., White D.D., White J.D., Wiley G.B.,
RA Wincker P., Xing Y., Yang L., Yao Z., Ying F., Zhai J., Zhou L., Zuber A.,
RA Denarie J., Dixon R.A., May G.D., Schwartz D.C., Rogers J., Quetier F.,
RA Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [2] {ECO:0000313|EMBL:AES62375.2, ECO:0000313|Proteomes:UP000002051}
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17 {ECO:0000313|EnsemblPlants:AES62375,
RC ECO:0000313|Proteomes:UP000002051};
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [3] {ECO:0000313|EnsemblPlants:AES62375}
RP IDENTIFICATION.
RC STRAIN=cv. Jemalong A17 {ECO:0000313|EnsemblPlants:AES62375};
RG EnsemblPlants;
RL Submitted (APR-2015) to UniProtKB.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Classic translation factor GTPase family. IF-2 subfamily.
CC {ECO:0000256|ARBA:ARBA00007733}.
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DR EMBL; CM001217; AES62375.2; -; Genomic_DNA.
DR RefSeq; XP_003592124.2; XM_003592076.2.
DR STRING; 3880.G7ID01; -.
DR PaxDb; 3880-AES62375; -.
DR EnsemblPlants; AES62375; AES62375; MTR_1g099000.
DR GeneID; 11422436; -.
DR Gramene; AES62375; AES62375; MTR_1g099000.
DR KEGG; mtr:11422436; -.
DR eggNOG; KOG1144; Eukaryota.
DR HOGENOM; CLU_002656_2_1_1; -.
DR OrthoDB; 169393at2759; -.
DR Proteomes; UP000002051; Chromosome 1.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-KW.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:InterPro.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003743; F:translation initiation factor activity; IBA:GO_Central.
DR GO; GO:0006413; P:translational initiation; IBA:GO_Central.
DR CDD; cd03703; aeIF5B_II; 1.
DR CDD; cd16266; IF2_aeIF5B_IV; 1.
DR CDD; cd01887; IF2_eIF5B; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 2.
DR Gene3D; 3.40.50.10050; Translation initiation factor IF- 2, domain 3; 1.
DR InterPro; IPR029459; EFTU-type.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005225; Small_GTP-bd_dom.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR015760; TIF_IF2.
DR InterPro; IPR023115; TIF_IF2_dom3.
DR InterPro; IPR036925; TIF_IF2_dom3_sf.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR43381:SF4; EUKARYOTIC TRANSLATION INITIATION FACTOR 5B; 1.
DR PANTHER; PTHR43381; TRANSLATION INITIATION FACTOR IF-2-RELATED; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF14578; GTP_EFTU_D4; 1.
DR Pfam; PF11987; IF-2; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF52156; Initiation factor IF2/eIF5b, domain 3; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 3: Inferred from homology;
KW Chloroplast {ECO:0000256|ARBA:ARBA00022528};
KW Initiation factor {ECO:0000256|ARBA:ARBA00022540,
KW ECO:0000313|EMBL:AES62375.2}; Plastid {ECO:0000256|ARBA:ARBA00022640};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000002051}.
FT DOMAIN 748..964
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
FT REGION 1..621
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 694..744
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 46..74
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 116..140
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 175..190
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 218..233
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 277..294
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 356..371
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 407..424
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 451..558
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 586..601
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 710..736
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1340 AA; 147183 MW; 2D84A6589F1A7D3B CRC64;
MGKKKPAIRD DENPPPQQGG GKSKKKAPVI DDDEYSIGVE VSEDAIPVAV EEKPVKKKGK
KGNAKSKKDN DDDFDDAVVV DDDDVNEIVF AGKKKGKSKK SSGGSVFASG FGVLGDEEDV
DNDDDEKSEV TGEGDDDEDE PVVSFTGKKK SSKGSKKGGG GSVFTAAAGF GLLGDDEDVD
NDDGEEKSDE DEPVVFSGKK KPSKGWKKGA SSLFSALDEN DDGEEESKNE KVEDDDDEPI
TFSGKKKKSS KGSKKAGVSL SKAIPEEDSV SVSESAKGGD YEKDEDDVSF AFTGKKKSSK
KKSGSAAAKV SDEIEFGSES VNVVEAEKPS VDNGNISKSE EVVGTSKNKK KNKKKSGRTK
EEEDDLDKLL AELGEAPATA QPAAAPPQQD DKVQPVPVVG SAPGASGEKE GEDETVESAA
TKKKKKKKEK EKEKKAAAAA AGSAPVVEAV EEKAEAIEPK KNDSKTKAAD KKVPKHVREM
QELLARRKEA EEKKKKEEEE KQRKEEEERR RIEELERQAE EAKRRKKEKE KEKLLKKKQE
GKLLTGKQKE EARRLEAMRR QILNSTGGVT LPGADTGGPS KKPIYQTKKG KSTNRNHNGA
AAVKTEENVE ATETTADLDT EELEKVEEVE SVQMEDKVEL PEVVEEVVDE DDDVEDEWDA
KSWDDVNLND RGAFADEEVD SEPEPIVKKE IKNGIPSKNA GATNKPVTKP AAEETEDRKQ
AKVVVEDKKK KHDPQLSAVP SKPSEGNLRS PICCIMGHVD TGKTKLLDCI RGTNVQEGEA
GGITQQIGAT YFPAENIRER TKELKADATL KVPGLLVIDT PGHESFNNLR SRGSGLCDIA
ILVVDIMHGL EPQTIESLNL LKMRNTEFIV ALNKVDRLYG WKTCRNAPIR KAMTQQSKDV
QNEFNMRVTQ IVTQFKEQGL NTELYYKNKE MGETFSIVPT SAISGEGIPD MLLLLVQWTQ
KTMTEKLTYS EEVQCTVLEV KVIEGHGTTI DVVLVNGVLH EGDQIVVSGM QGPIVTTIRA
LLTPHPMKEL RVKGSYIHHK EIKAAMGIKI TAQGLEHAIA GASLYVVKPD DDLEYIKKAA
LEDVESVLSR IDRSGEGVCV QASTLGSLEA LLEFLKTPEV NIPVSAINIG PVHKKDVMKA
SVMLEKKREY ATILAFDVKV TPEARDLAEE LGVKIFIADI IYHLFDQFKA YMDNIKEEKK
KESADEAVFP CVLKILPNCV FNKKDPIVLG VDILEGILKI GTPICIPSQE FIDIGRIASI
ENNHKPVDYA KKGQKVAIKI VGSNSEEQQK MFGRHFEIDD ELVSHISRRS IDILKTNYRD
DLTMEEWKLV VKLKTLFKIQ
//
