ID G7JB32_MEDTR Unreviewed; 1203 AA.
AC G7JB32; A0A0C3VQL2;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 01-OCT-2014, sequence version 2.
DT 24-JAN-2024, entry version 69.
DE RecName: Full=RNA-dependent RNA polymerase {ECO:0000256|RuleBase:RU363098};
DE EC=2.7.7.48 {ECO:0000256|RuleBase:RU363098};
GN Name=11431963 {ECO:0000313|EnsemblPlants:AES73660};
GN OrderedLocusNames=MTR_3g107390 {ECO:0000313|EMBL:AES73660.2};
GN ORFNames=MtrunA17_Chr3g0137841 {ECO:0000313|EMBL:RHN70648.1};
OS Medicago truncatula (Barrel medic) (Medicago tribuloides).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Medicago.
OX NCBI_TaxID=3880 {ECO:0000313|EMBL:AES73660.2, ECO:0000313|Proteomes:UP000002051};
RN [1] {ECO:0000313|EMBL:AES73660.2, ECO:0000313|Proteomes:UP000002051}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=A17 {ECO:0000313|EMBL:AES73660.2}, and cv. Jemalong A17
RC {ECO:0000313|EnsemblPlants:AES73660,
RC ECO:0000313|Proteomes:UP000002051};
RX PubMed=22089132; DOI=10.1038/nature10625;
RA Young N.D., Debelle F., Oldroyd G.E., Geurts R., Cannon S.B., Udvardi M.K.,
RA Benedito V.A., Mayer K.F., Gouzy J., Schoof H., Van de Peer Y., Proost S.,
RA Cook D.R., Meyers B.C., Spannagl M., Cheung F., De Mita S.,
RA Krishnakumar V., Gundlach H., Zhou S., Mudge J., Bharti A.K., Murray J.D.,
RA Naoumkina M.A., Rosen B., Silverstein K.A., Tang H., Rombauts S.,
RA Zhao P.X., Zhou P., Barbe V., Bardou P., Bechner M., Bellec A., Berger A.,
RA Berges H., Bidwell S., Bisseling T., Choisne N., Couloux A., Denny R.,
RA Deshpande S., Dai X., Doyle J.J., Dudez A.M., Farmer A.D., Fouteau S.,
RA Franken C., Gibelin C., Gish J., Goldstein S., Gonzalez A.J., Green P.J.,
RA Hallab A., Hartog M., Hua A., Humphray S.J., Jeong D.H., Jing Y.,
RA Jocker A., Kenton S.M., Kim D.J., Klee K., Lai H., Lang C., Lin S.,
RA Macmil S.L., Magdelenat G., Matthews L., McCorrison J., Monaghan E.L.,
RA Mun J.H., Najar F.Z., Nicholson C., Noirot C., O'Bleness M., Paule C.R.,
RA Poulain J., Prion F., Qin B., Qu C., Retzel E.F., Riddle C., Sallet E.,
RA Samain S., Samson N., Sanders I., Saurat O., Scarpelli C., Schiex T.,
RA Segurens B., Severin A.J., Sherrier D.J., Shi R., Sims S., Singer S.R.,
RA Sinharoy S., Sterck L., Viollet A., Wang B.B., Wang K., Wang M., Wang X.,
RA Warfsmann J., Weissenbach J., White D.D., White J.D., Wiley G.B.,
RA Wincker P., Xing Y., Yang L., Yao Z., Ying F., Zhai J., Zhou L., Zuber A.,
RA Denarie J., Dixon R.A., May G.D., Schwartz D.C., Rogers J., Quetier F.,
RA Town C.D., Roe B.A.;
RT "The Medicago genome provides insight into the evolution of rhizobial
RT symbioses.";
RL Nature 480:520-524(2011).
RN [2] {ECO:0000313|EMBL:AES73660.2, ECO:0000313|Proteomes:UP000002051}
RP GENOME REANNOTATION.
RC STRAIN=cv. Jemalong A17 {ECO:0000313|EnsemblPlants:AES73660,
RC ECO:0000313|Proteomes:UP000002051};
RX PubMed=24767513; DOI=10.1186/1471-2164-15-312;
RA Tang H., Krishnakumar V., Bidwell S., Rosen B., Chan A., Zhou S.,
RA Gentzbittel L., Childs K.L., Yandell M., Gundlach H., Mayer K.F.,
RA Schwartz D.C., Town C.D.;
RT "An improved genome release (version Mt4.0) for the model legume Medicago
RT truncatula.";
RL BMC Genomics 15:312-312(2014).
RN [3] {ECO:0000313|EnsemblPlants:AES73660}
RP IDENTIFICATION.
RC STRAIN=cv. Jemalong A17 {ECO:0000313|EnsemblPlants:AES73660};
RG EnsemblPlants;
RL Submitted (APR-2015) to UniProtKB.
RN [4] {ECO:0000313|Proteomes:UP000265566}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Jemalong A17 {ECO:0000313|Proteomes:UP000265566};
RX PubMed=30397259; DOI=10.1038/s41477-018-0286-7;
RA Pecrix Y., Staton S.E., Sallet E., Lelandais-Briere C., Moreau S.,
RA Carrere S., Blein T., Jardinaud M.F., Latrasse D., Zouine M., Zahm M.,
RA Kreplak J., Mayjonade B., Satge C., Perez M., Cauet S., Marande W.,
RA Chantry-Darmon C., Lopez-Roques C., Bouchez O., Berard A., Debelle F.,
RA Munos S., Bendahmane A., Berges H., Niebel A., Buitink J., Frugier F.,
RA Benhamed M., Crespi M., Gouzy J., Gamas P.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 4:1017-1025(2018).
RN [5] {ECO:0000313|EMBL:RHN70648.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaves {ECO:0000313|EMBL:RHN70648.1};
RA Pecrix Y., Gamas P., Carrere S.;
RT "Whole-genome landscape of Medicago truncatula symbiotic genes.";
RL Nat. Plants 0:0-0(2018).
CC -!- FUNCTION: Probably involved in the RNA silencing pathway and required
CC for the generation of small interfering RNAs (siRNAs).
CC {ECO:0000256|RuleBase:RU363098}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.48; Evidence={ECO:0000256|ARBA:ARBA00024517,
CC ECO:0000256|RuleBase:RU363098};
CC -!- SIMILARITY: Belongs to the RdRP family. {ECO:0000256|ARBA:ARBA00005762,
CC ECO:0000256|RuleBase:RU363098}.
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DR EMBL; CM001219; AES73660.2; -; Genomic_DNA.
DR EMBL; PSQE01000003; RHN70648.1; -; Genomic_DNA.
DR RefSeq; XP_003603409.2; XM_003603361.2.
DR AlphaFoldDB; G7JB32; -.
DR STRING; 3880.G7JB32; -.
DR PaxDb; 3880-AES73660; -.
DR EnsemblPlants; AES73660; AES73660; MTR_3g107390.
DR Gramene; AES73660; AES73660; MTR_3g107390.
DR eggNOG; KOG0988; Eukaryota.
DR HOGENOM; CLU_001366_3_1_1; -.
DR OrthoDB; 5477883at2759; -.
DR Proteomes; UP000002051; Chromosome 3.
DR Proteomes; UP000265566; Chromosome 3.
DR GO; GO:0031380; C:nuclear RNA-directed RNA polymerase complex; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IBA:GO_Central.
DR GO; GO:0030422; P:siRNA processing; IBA:GO_Central.
DR CDD; cd00590; RRM_SF; 1.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR007855; RNA-dep_RNA_pol_euk-typ.
DR PANTHER; PTHR23079; RNA-DEPENDENT RNA POLYMERASE; 1.
DR PANTHER; PTHR23079:SF18; RNA-DEPENDENT RNA POLYMERASE 6; 1.
DR Pfam; PF05183; RdRP; 1.
DR SUPFAM; SSF54928; RNA-binding domain, RBD; 1.
PE 3: Inferred from homology;
KW Nucleotidyltransferase {ECO:0000256|RuleBase:RU363098,
KW ECO:0000313|EMBL:RHN70648.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000002051};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|RuleBase:RU363098};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484,
KW ECO:0000256|RuleBase:RU363098};
KW RNA-mediated gene silencing {ECO:0000256|RuleBase:RU363098};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU363098}.
SQ SEQUENCE 1203 AA; 136906 MW; F178898F038872D7 CRC64;
MDLEGSVKDS VLTTTQVSIG GFDSDVKASD LVSYLESKIG YVDRCRLKTS CTPPESYPEF
TMTDTKQIEI TDDYKKVAPH AFVHFTLPDS ATEALNAAGR CDLILNGKVL KVISGPQNPN
FLNQRRRTEA PFKMSDVIVE IGTLVSPADF TVAWRGCDKG VNFLVDPFDK ICKICFNRDT
AFSFKGIGKQ AVIKCDFKVG FLIRDIEEIQ RYDDTSHLVV LLRLASSPLV WYRTADDDID
ESVTIDLLDD DDPWIRTTDF TPSGAIGRCN YYRISIPPRH GAKLNKALEY LRRQRVPRIP
LKRPPKIQNE PDFGVPMSDA FFYIDSQKDI SFDIMFLINA IVHRGIFNQY SLSNKFFELL
KNQPKDVNVA ALKHICTYKH PVFDAAKRLE AVQKWLLRNP KFYQISKQSD DVMEVRRLVV
TPTKAYCISP EVELSNRVLR RFREVSDRFL RVTFMDEGQQ TLNNYALNYY VAPIVKEITS
NSFQQKTRIY KRVKTILDDG FYFCGRKYSF LAFSSNQLRD RSAWFFAEDK DITCDDIRNW
MGRFNQKNVA KCAARMGQCF SSTYASVEVP ANEVNSMLPD VERNNYVFSD GIGIITTDLA
VEVAEKLKLD KAPSAYQIRY AGFKGVVACW PAKGDGIKLS LRPSMNKFQS THTTLEICAW
TRFQPGFLNR QIITLLSALD VPDEIFWKMQ EGMISSLNQM LVDRDVAYDV LTKSCAEHGN
AAAIMLSCGF SPKTEPHLRG MLNSIRAAQL WGLREKSRIF VSSGRWLMGV LDELGVLEQG
QCFVQVSTPS LENCFSKHGS RFSETKTVHV VKGIVVIAKN PCLHPGDVRV LEAVDVPDLH
HLYDCVVFPQ KGERPHTDEA SGSDLDGDLY FVTWDGNLIP PSKRSWPPME YAAQESRLQT
RQVTVQDIID FFVRNMVNEQ LGSICNAHVV HADSSDYGAL DENCVHLAEL AATAVDFPKT
GKLVTMPSNL KPKLYPDFMG KEHHQSYKSK KILGRLYRRI KDAYDKDIDA PEFNCVSSDI
LYDTDLEVPG SSEFIADAWE QRCSYDGQLS GLLGQYKVKT EEEVVTGQIW SMPKYNSRKQ
GELKERLKHS YSALKKEFRQ IFEKLKSDVG ELSDKEKNLL YEQKASAWYQ VTYHPKWVKK
SLDLQLKSSD NQSADSLGNM VMLSFPWIAV DYLACTKTRH RRVKDFDSTK QVDSLAKYLS
ERL
//
