ID G7LUE0_9GAMM Unreviewed; 416 AA.
AC G7LUE0;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=Lipid III flippase {ECO:0000256|HAMAP-Rule:MF_02024};
GN Name=wzxE {ECO:0000256|HAMAP-Rule:MF_02024};
GN ORFNames=BrE312_0256 {ECO:0000313|EMBL:EHD19713.1};
OS Brenneria sp. EniD312.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Pectobacteriaceae; Brenneria.
OX NCBI_TaxID=598467 {ECO:0000313|EMBL:EHD19713.1};
RN [1] {ECO:0000313|EMBL:EHD19713.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EniD312 {ECO:0000313|EMBL:EHD19713.1};
RG US DOE Joint Genome Institute;
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Mikhailova N., Monk A.C., Detter J.C., Han C., Tapia R.,
RA Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Balakrishnan V.,
RA Glasner J., Perna N., Woyke T.;
RT "Complete sequence of Brenneria sp. EniD312.";
RL Submitted (JUL-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Mediates the transbilayer movement of Und-PP-GlcNAc-ManNAcA-
CC Fuc4NAc (lipid III) from the inner to the outer leaflet of the
CC cytoplasmic membrane during the assembly of enterobacterial common
CC antigen (ECA). {ECO:0000256|HAMAP-Rule:MF_02024}.
CC -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC antigen biosynthesis. {ECO:0000256|HAMAP-Rule:MF_02024}.
CC -!- SUBUNIT: Probably part of a complex composed of WzxE, WzyE and WzzE.
CC {ECO:0000256|HAMAP-Rule:MF_02024}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000256|HAMAP-
CC Rule:MF_02024}; Multi-pass membrane protein {ECO:0000256|HAMAP-
CC Rule:MF_02024}. Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-pass
CC membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the polysaccharide transport (PST) (TC 2.A.66.2)
CC family. {ECO:0000256|HAMAP-Rule:MF_02024}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CM001230; EHD19713.1; -; Genomic_DNA.
DR RefSeq; WP_009111033.1; NZ_CM001230.1.
DR AlphaFoldDB; G7LUE0; -.
DR STRING; 598467.BrE312_0256; -.
DR eggNOG; COG2244; Bacteria.
DR HOGENOM; CLU_042154_0_0_6; -.
DR UniPathway; UPA00566; -.
DR Proteomes; UP000002759; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd13125; MATE_like_10; 1.
DR HAMAP; MF_02024; WzxE; 1.
DR InterPro; IPR002797; Polysacc_synth.
DR InterPro; IPR044550; WzxE.
DR InterPro; IPR032896; WzxE_Proteobacteria.
DR PANTHER; PTHR30250:SF30; LIPID III FLIPPASE; 1.
DR PANTHER; PTHR30250; PST FAMILY PREDICTED COLANIC ACID TRANSPORTER; 1.
DR Pfam; PF01943; Polysacc_synt; 1.
PE 3: Inferred from homology;
KW Cell inner membrane {ECO:0000256|HAMAP-Rule:MF_02024};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475, ECO:0000256|HAMAP-
KW Rule:MF_02024};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|HAMAP-Rule:MF_02024};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|HAMAP-
KW Rule:MF_02024};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989, ECO:0000256|HAMAP-
KW Rule:MF_02024}; Transport {ECO:0000256|HAMAP-Rule:MF_02024}.
FT TRANSMEM 12..33
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 45..67
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 79..106
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 118..139
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 146..165
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 171..195
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 216..237
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 257..278
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 299..322
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 334..355
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 362..382
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
FT TRANSMEM 394..412
FT /note="Helical"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02024"
SQ SEQUENCE 416 AA; 44914 MW; B714655912F102D5 CRC64;
MSLAKASVWT AGSTLIKIGA GLVVVKLLAV AFGPAGVGQA GNFRQLITVL GVLSGAGIFN
GVTKYVAEYQ QQPDRLRPLL GTSVALVLGF STLLAAVFVL AAAPIAHALF GHDEYREVIR
ALALIQMAIA YANLFLAILK GYRDAVGNAL AVIGGSLIGL AAYWLCFTLG GYRGALAGLA
LVPALAALPA GWLMLRRTPL TLAWLKPAWD KAMAGHLGKF TLMALMTAVT LPVAYIMMRN
LLAAHYGWDD VGIWQGVSTI SDAYLQFITA SFTVYLLPTL SRLTDKTLLA QEIVRSLKFV
LPAVAAVSLG VWLLRDFAIW LLFSSQFTAM RDLFAWQLVG DVMKVGAYVF GYLVIAKAAL
RFYLLTEVSQ FLLLTGFAHW LIPVNGALGA AQAYMATYLV YFLLCCSVFI IYRRRA
//
