ID G7M4W9_9CLOT Unreviewed; 281 AA.
AC G7M4W9;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE SubName: Full=2,5-didehydrogluconate reductase {ECO:0000313|EMBL:EHI99189.1};
DE EC=1.1.1.274 {ECO:0000313|EMBL:EHI99189.1};
GN ORFNames=CDLVIII_2577 {ECO:0000313|EMBL:EHI99189.1};
OS Clostridium sp. DL-VIII.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=641107 {ECO:0000313|EMBL:EHI99189.1, ECO:0000313|Proteomes:UP000005106};
RN [1] {ECO:0000313|EMBL:EHI99189.1, ECO:0000313|Proteomes:UP000005106}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DL-VIII {ECO:0000313|EMBL:EHI99189.1,
RC ECO:0000313|Proteomes:UP000005106};
RX PubMed=23929491;
RA Taghavi S., Izquierdo J.A., van der Lelie D.;
RT "Complete Genome Sequence of Clostridium sp. Strain DL-VIII, a Novel
RT Solventogenic Clostridium Species Isolated from Anaerobic Sludge.";
RL Genome Announc. 1:e00605-e00613(2013).
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CM001240; EHI99189.1; -; Genomic_DNA.
DR RefSeq; WP_009169861.1; NZ_CM001240.1.
DR AlphaFoldDB; G7M4W9; -.
DR STRING; 641107.CDLVIII_2577; -.
DR eggNOG; COG0656; Bacteria.
DR HOGENOM; CLU_023205_0_1_9; -.
DR Proteomes; UP000005106; Chromosome.
DR GO; GO:0050580; F:2,5-didehydrogluconate reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR GO; GO:0044281; P:small molecule metabolic process; IEA:UniProt.
DR CDD; cd19133; AKR_AKR5F1; 1.
DR Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR InterPro; IPR020471; AKR.
DR InterPro; IPR018170; Aldo/ket_reductase_CS.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR PANTHER; PTHR43827; 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE; 1.
DR PANTHER; PTHR43827:SF3; ALDO-KETO REDUCTASE; 1.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR PIRSF; PIRSF000097; AKR; 2.
DR PRINTS; PR00069; ALDKETRDTASE.
DR SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
DR PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE 4: Predicted;
KW Oxidoreductase {ECO:0000313|EMBL:EHI99189.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000005106}.
FT DOMAIN 23..255
FT /note="NADP-dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF00248"
FT ACT_SITE 49
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-1"
FT BINDING 107
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-2"
FT SITE 74
FT /note="Lowers pKa of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-3"
SQ SEQUENCE 281 AA; 32103 MW; 530B0D3D843F73A6 CRC64;
MKYVKLNNDV KMPILGYGVF QIPDQEECER CVLDAIEVGY RLIDTAQAYG NEEAVGKAIK
KCGVPREGLF ITTKVWIANA GYENAKKSIE ESLKKLQLDY LDLVLIHQPF NDYYGTYRAM
EELYKEGKLK AIGVSNFYPD RLIDLIKFNE VVPAVNQVET HVFNQQVKAQ EVMKKYGVQI
QAWAPFAEGK NNLFSNETLK AVGDKYNKSI AQVALRYLIQ RGVSVLPKSV SKERMIQNID
VFDFELTKED MDLIAVLDKA ESLFFSHYDP QTVEYLTGLV R
//
