ID G7PAM2_MACFA Unreviewed; 1053 AA.
AC G7PAM2;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE RecName: Full=Fanconi-associated nuclease {ECO:0000256|RuleBase:RU365033};
DE EC=3.1.4.1 {ECO:0000256|RuleBase:RU365033};
GN ORFNames=EGM_15799 {ECO:0000313|EMBL:EHH62932.1};
OS Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini;
OC Cercopithecidae; Cercopithecinae; Macaca.
OX NCBI_TaxID=9541;
RN [1] {ECO:0000313|EMBL:EHH62932.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CE-4 {ECO:0000313|EMBL:EHH62932.1};
RX PubMed=22002653; DOI=10.1038/nbt.1992;
RA Yan G., Zhang G., Fang X., Zhang Y., Li C., Ling F., Cooper D.N., Li Q.,
RA Li Y., van Gool A.J., Du H., Chen J., Chen R., Zhang P., Huang Z.,
RA Thompson J.R., Meng Y., Bai Y., Wang J., Zhuo M., Wang T., Huang Y.,
RA Wei L., Li J., Wang Z., Hu H., Yang P., Le L., Stenson P.D., Li B., Liu X.,
RA Ball E.V., An N., Huang Q., Zhang Y., Fan W., Zhang X., Li Y., Wang W.,
RA Katze M.G., Su B., Nielsen R., Yang H., Wang J., Wang X., Wang J.;
RT "Genome sequencing and comparison of two nonhuman primate animal models,
RT the cynomolgus and Chinese rhesus macaques.";
RL Nat. Biotechnol. 29:1019-1023(2011).
CC -!- FUNCTION: Nuclease required for the repair of DNA interstrand cross-
CC links (ICL). Acts as a 5'-3' exonuclease that anchors at a cut end of
CC DNA and cleaves DNA successively at every third nucleotide, allowing to
CC excise an ICL from one strand through flanking incisions.
CC {ECO:0000256|RuleBase:RU365033}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolytically removes 5'-nucleotides successively from the
CC 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides.;
CC EC=3.1.4.1; Evidence={ECO:0000256|ARBA:ARBA00000983,
CC ECO:0000256|RuleBase:RU365033};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU365033};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|RuleBase:RU365033};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|RuleBase:RU365033}.
CC -!- SIMILARITY: Belongs to the FAN1 family. {ECO:0000256|ARBA:ARBA00005533,
CC ECO:0000256|RuleBase:RU365033}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CM001282; EHH62932.1; -; Genomic_DNA.
DR AlphaFoldDB; G7PAM2; -.
DR eggNOG; KOG2143; Eukaryota.
DR Proteomes; UP000009130; Chromosome 7.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0004528; F:phosphodiesterase I activity; IEA:UniProtKB-EC.
DR GO; GO:0036297; P:interstrand cross-link repair; IEA:InterPro.
DR CDD; cd22326; FAN1-like; 1.
DR Gene3D; 3.40.1350.10; -; 1.
DR InterPro; IPR033315; Fan1-like.
DR InterPro; IPR049132; FAN1-like_euk.
DR InterPro; IPR049126; FAN1-like_TPR.
DR InterPro; IPR049125; FAN1-like_WH.
DR InterPro; IPR049138; Fan1_SAP_met.
DR InterPro; IPR011856; tRNA_endonuc-like_dom_sf.
DR InterPro; IPR014883; VRR_NUC.
DR PANTHER; PTHR15749; FANCONI-ASSOCIATED NUCLEASE 1; 1.
DR PANTHER; PTHR15749:SF4; FANCONI-ASSOCIATED NUCLEASE 1; 1.
DR Pfam; PF21315; FAN1_HTH; 1.
DR Pfam; PF21169; Fan1_SAP; 1.
DR Pfam; PF21170; FAN1_TPR; 2.
DR Pfam; PF08774; VRR_NUC; 1.
DR SMART; SM00990; VRR_NUC; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|RuleBase:RU365033};
KW DNA repair {ECO:0000256|RuleBase:RU365033};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU365033};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|RuleBase:RU365033};
KW Manganese {ECO:0000256|ARBA:ARBA00023211, ECO:0000256|RuleBase:RU365033};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU365033};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|RuleBase:RU365033};
KW Nucleus {ECO:0000256|RuleBase:RU365033}.
FT DOMAIN 929..1044
FT /note="VRR-NUC"
FT /evidence="ECO:0000259|SMART:SM00990"
FT REGION 104..123
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 170..201
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 246..281
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 104..118
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 182..196
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 252..269
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1053 AA; 116464 MW; E3FBACA33591E570 CRC64;
MHLAIDIAAH SLATVQFLPF QFRDVVEKVE YRPEPGWFSP GVKQDLAPQA LPVQALPELI
LLCSCHGPGR CESQASLPMW DGKGVLLELM LKIFVSLPPI SLKSQGSNPY NDPQGHTSGA
DTHKMVPRYD LNRHLDEMCA NSDFVQVDPG QVGLINSNVS TVDLTSVTLE DVTPKKSPPP
KRNLTPGQSD SAKMDAKQKT SPYFKSKDGV VCKHQDTLRN RSVKVICLGS LASKLSRKYV
KAKKSIDKDE EFAGSSPQSS RSTVDKSLVD NSSEIEDEDQ ILQNSSQKEN VFVCDSLKEE
CIPEHMIRGS KIMEAKNQKA TQECEKSALS PGFSDNAIML FSPDFTLGNT LKSTSEDSLV
KQECIQGVVE KREACREEVK MTVASEAKMQ LSDSEAKSHS SADDASAWSN IQEPPLQDNS
CLNSDIPCSI PLEQGSSCDG PGQTTGHPYY LRSFLVVLKT VLENEDDMML FDEQEKGIIT
QFYQLSVTGQ KLYVRLFQRK LSWIKMTKLE YEEIASDLTP VIEELKNAGF LQTESELQEL
SEVLELLSAP ELKSLAKTFH LVNPNGQKQQ LVDAFLKLAK QRSVCTWGKN KPGIGAVILK
RAKALAGQSI RICKGPRAVF SRILLLFSLT DSMEDEDAAC GGQGQLSTVL LVNLGRMEFP
TYTINRKTQI FQDRDDLIRY AAATHMLSDI SSAMANGNWE EAKELAQCAK RDWNRLKNHP
SLSIEKKVKT DSCSVCLCVD ESVSQDSLGV AASILEGAPQ TIKCITEGLA DPEVRTGHRL
SLYQRAVRLR ESPSCKKFRR LFQQLPEMAV QDVKHVTITG RLCPQRGMGK SVFVMEAGEA
ADPTTVLCSV EELALAHYRR SGFDQGIHGE GSTFSTLCGL LLWDIIFMDG IPDVFRNACQ
AFPLDLCTDS FFASRRPALE ARLQLIHDAP AESLRAWVAA TWQEQEGRVA SLVSWDRFTS
LQQAQDLVSC LGGPVLSGVC RRLAADFRHC RGGLPDLVVW NSQSHRFKLV EVKGPNDRLS
HKQMIWLAEL QKLGAEVEVC HVVAVGAKSQ SLS
//
