ID G7UVU4_PSEUP Unreviewed; 871 AA.
AC G7UVU4;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=aconitate hydratase {ECO:0000256|ARBA:ARBA00012926};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
GN Name=acnA {ECO:0000313|EMBL:AER56413.1};
GN OrderedLocusNames=DSC_08815 {ECO:0000313|EMBL:AER56413.1};
OS Pseudoxanthomonas spadix (strain BD-a59).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Pseudoxanthomonas.
OX NCBI_TaxID=1045855 {ECO:0000313|EMBL:AER56413.1, ECO:0000313|Proteomes:UP000005870};
RN [1] {ECO:0000313|EMBL:AER56413.1, ECO:0000313|Proteomes:UP000005870}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BD-a59 {ECO:0000313|EMBL:AER56413.1,
RC ECO:0000313|Proteomes:UP000005870};
RX PubMed=22207748; DOI=10.1128/JB.06436-11;
RA Lee S.H., Jin H.M., Lee H.J., Kim J.M., Jeon C.O.;
RT "Complete Genome Sequence of the BTEX-Degrading Bacterium Pseudoxanthomonas
RT spadix BD-a59.";
RL J. Bacteriol. 194:544-544(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the aconitase/IPM isomerase family.
CC {ECO:0000256|ARBA:ARBA00007185}.
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DR EMBL; CP003093; AER56413.1; -; Genomic_DNA.
DR RefSeq; WP_014160589.1; NC_016147.2.
DR AlphaFoldDB; G7UVU4; -.
DR STRING; 1045855.DSC_08815; -.
DR KEGG; psd:DSC_08815; -.
DR eggNOG; COG1048; Bacteria.
DR HOGENOM; CLU_013476_2_1_6; -.
DR OrthoDB; 9764318at2; -.
DR Proteomes; UP000005870; Chromosome.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019679; P:propionate metabolic process, methylcitrate cycle; IEA:InterPro.
DR Gene3D; 6.10.190.10; -; 1.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 2.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR012708; 2Me_IsoCit_deHydtase_FeS-dep.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR006249; Aconitase/IRP2.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR NCBIfam; TIGR02333; 2met_isocit_dHY; 1.
DR PANTHER; PTHR11670:SF43; 2-METHYLCITRATE DEHYDRATASE (2-METHYL-TRANS-ACONITATE FORMING); 1.
DR PANTHER; PTHR11670; ACONITASE/IRON-RESPONSIVE ELEMENT FAMILY MEMBER; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR PRINTS; PR00415; ACONITASE.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000005870}.
FT DOMAIN 66..540
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 664..795
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 871 AA; 94638 MW; F167BDA94F31343E CRC64;
MNSAFRKPLP GTDLDYFDAR AAVEALQPGA WDTLPYTSRV HAENLVRRCD PALLDDALQQ
LIQRRRDLDF PWFPARVVCH DILGQTALVD LAGLRDAIAS QGGDPAQVNP VVPTQLIVDH
SLAVEYAGFD RNAFAKNRAV EDRRNEDRFH FIDWTSRAFK NVDVIPPGNG IMHQINLERM
SPVVHAVDGV AYPDTLVGTD SHTPHVDALG VIAIGVGGLE AESVMLGRAS WMRLPDIIGV
ELTGRPQPGI TATDVVLALT EFLRKEKVVG AYLEFFGQGA DALTIGDRAT ISNMTPEFGA
TAAMFYIDQQ TIDYLRLTGR EDAQVKLVQT YAKAAGLWAD ALKTAQYERV LRFDLSSVVR
NMAGPSNPHR RVATTELAAR GIADEAKLEA GKAEQAKGLM PDGAVIIAAI TSCTNTSNPR
NVIAAGLLAR NANARGLTRK PWVKSSLAPG SKAVQLYLED AKLLSELEQL GFGIVAFACT
TCNGMSGALD PAIQQEIIDR DLYATAVLSG NRNFDGRIHP YAKQAFLASP PLVVAYAIAG
TVRFDIEKDV LGLDQAGNPV TLKDIWPSDA QIDAIVAQSV KPEHFRRVYD PMFARTAHSG
ARIDPLYAWR PRSTYIRRPP YWEGALAGAR TLRGMRPLAV LGDNITTDHL SPSNAILPDS
AAGEYLAKMG VPEEDFNSYA THRGDHLTAQ RATFANPKLI NEMAVVDGQV KQGSLARIEP
EGKVTRMWEA IETYMARKQP LIIIAGADYG QGSSRDWAAK GVRLAGVEAI VAEGFERIHR
TNLVGMGVLP LEFKPGTDRK TLGIDGTETF DVVGTPTPRA DLTLVIHRKD GSQQQVAVTC
RLDTAEEVAI YEAGGVLQRF AQDFLASSKA A
//
