ID G7WH48_DESOD Unreviewed; 569 AA.
AC G7WH48;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE SubName: Full=Ni,Fe-hydrogenase I large subunit {ECO:0000313|EMBL:AET69556.1};
GN OrderedLocusNames=Desor_4119 {ECO:0000313|EMBL:AET69556.1};
OS Desulfosporosinus orientis (strain ATCC 19365 / DSM 765 / NCIMB 8382 / VKM
OS B-1628 / Singapore I) (Desulfotomaculum orientis).
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Desulfitobacteriaceae;
OC Desulfosporosinus.
OX NCBI_TaxID=768706 {ECO:0000313|EMBL:AET69556.1, ECO:0000313|Proteomes:UP000006346};
RN [1] {ECO:0000313|Proteomes:UP000006346}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628
RC {ECO:0000313|Proteomes:UP000006346};
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Ovchinnikova G., Teshima H., Detter J.C., Han C., Tapia R.,
RA Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Pester M.,
RA Spring S., Ollivier B., Rattei T., Klenk H.-P., Wagner M., Loy A.,
RA Woyke T.;
RT "Complete sequence of Desulfosporosinus orientis DSM 765.";
RL Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AET69556.1, ECO:0000313|Proteomes:UP000006346}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 19365 / DSM 765 / NCIMB 8382 / VKM B-1628
RC {ECO:0000313|Proteomes:UP000006346};
RX PubMed=23105050; DOI=10.1128/JB.01392-12;
RA Pester M., Brambilla E., Alazard D., Rattei T., Weinmaier T., Han J.,
RA Lucas S., Lapidus A., Cheng J.F., Goodwin L., Pitluck S., Peters L.,
RA Ovchinnikova G., Teshima H., Detter J.C., Han C.S., Tapia R., Land M.L.,
RA Hauser L., Kyrpides N.C., Ivanova N.N., Pagani I., Huntmann M., Wei C.L.,
RA Davenport K.W., Daligault H., Chain P.S., Chen A., Mavromatis K.,
RA Markowitz V., Szeto E., Mikhailova N., Pati A., Wagner M., Woyke T.,
RA Ollivier B., Klenk H.P., Spring S., Loy A.;
RT "Complete genome sequences of Desulfosporosinus orientis DSM765T,
RT Desulfosporosinus youngiae DSM17734T, Desulfosporosinus meridiei DSM13257T,
RT and Desulfosporosinus acidiphilus DSM22704T.";
RL J. Bacteriol. 194:6300-6301(2012).
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000256|ARBA:ARBA00001967,
CC ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- SUBUNIT: Heterodimer of a large and a small subunit.
CC {ECO:0000256|ARBA:ARBA00011771}.
CC -!- SIMILARITY: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit
CC family. {ECO:0000256|ARBA:ARBA00009292, ECO:0000256|RuleBase:RU003896}.
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DR EMBL; CP003108; AET69556.1; -; Genomic_DNA.
DR RefSeq; WP_014186363.1; NC_016584.1.
DR AlphaFoldDB; G7WH48; -.
DR STRING; 768706.Desor_4119; -.
DR KEGG; dor:Desor_4119; -.
DR PATRIC; fig|768706.3.peg.4172; -.
DR eggNOG; COG0374; Bacteria.
DR HOGENOM; CLU_030087_0_0_9; -.
DR OrthoDB; 9761717at2; -.
DR Proteomes; UP000006346; Chromosome.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:InterPro.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR Gene3D; 1.10.645.10; Cytochrome-c3 Hydrogenase, chain B; 1.
DR InterPro; IPR001501; Ni-dep_hyd_lsu.
DR InterPro; IPR018194; Ni-dep_hyd_lsu_Ni_BS.
DR InterPro; IPR029014; NiFe-Hase_large.
DR PANTHER; PTHR42958; HYDROGENASE-2 LARGE CHAIN; 1.
DR PANTHER; PTHR42958:SF2; UPTAKE HYDROGENASE LARGE SUBUNIT; 1.
DR Pfam; PF00374; NiFeSe_Hases; 1.
DR SUPFAM; SSF56762; HydB/Nqo4-like; 1.
DR PROSITE; PS00507; NI_HGENASE_L_1; 1.
DR PROSITE; PS00508; NI_HGENASE_L_2; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|PIRSR:PIRSR601501-1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR601501-1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR601501-1};
KW Nickel {ECO:0000256|ARBA:ARBA00022596, ECO:0000256|PIRSR:PIRSR601501-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|RuleBase:RU003896};
KW Reference proteome {ECO:0000313|Proteomes:UP000006346}.
FT BINDING 41
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 60
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 63
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 63
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 548
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 551
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 554
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
SQ SEQUENCE 569 AA; 63504 MW; 7BFF98609EBEDF2C CRC64;
MTRIVVDPIT RIEGHLRIEA VVEGTRIVEA MSSGTVFRGI EKIVENRDPR DVWAFVQRIC
GVCTHIHALA SIRAVEDALD IRIPKNANYI RNIMTATQFV QDHVIHFYHL HGFDWIDVVS
ALKADPVQTS ALARSISSWP NSSDGYFRDV QAKVKTLVES GQLGIFANAY WGHPAYRLPP
EANLLGVAHY LDALTWQKEI AKIHTIFGGK NPHPNYLVGG MASTLNMNDN SVINMERLNL
VKTKIDEAQV FVDQVLIPDI FAIANFYKKD LFGGGVGNYL AYGGIPIVDI REPDSFLFPS
GVVLNRDLSK VLEVDLKDPK QIQEFVSHSW YRYDNTNAGL HPWQGQTEPA YDGPRPPYDN
LEENKGYSWI KEPRWQGHSM EVGPLARFVI AYARGNKEIK GMVDEALKRL DLPISAVFST
LGRTLARALE SKYLVNHLAG FYADLVDNIK AGDSQTFNGE KWDPKKWPAQ AQGVGFAEAP
RGALGHWVKI NNGKVSAYQA VVPTTWNASP RDDSGQKGPY ESSLLDTPIA IEDQPLELLR
TIHSFDPCLA CATHLITPKG QEIAKVRVF
//
