ID   G7ZAJ6_AZOL4            Unreviewed;       839 AA.
AC   G7ZAJ6;
DT   25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT   25-JAN-2012, sequence version 1.
DT   24-JAN-2024, entry version 52.
DE   RecName: Full=cellulase {ECO:0000256|ARBA:ARBA00012601};
DE            EC=3.2.1.4 {ECO:0000256|ARBA:ARBA00012601};
GN   OrderedLocusNames=AZOLI_p10561 {ECO:0000313|EMBL:CBS88800.1};
OS   Azospirillum lipoferum (strain 4B).
OG   Plasmid AZO_p1 {ECO:0000313|EMBL:CBS88800.1,
OG   ECO:0000313|Proteomes:UP000005667}.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC   Azospirillaceae; Azospirillum.
OX   NCBI_TaxID=862719 {ECO:0000313|EMBL:CBS88800.1, ECO:0000313|Proteomes:UP000005667};
RN   [1] {ECO:0000313|Proteomes:UP000005667}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=4B {ECO:0000313|Proteomes:UP000005667};
RC   PLASMID=Plasmid AZO_p1 {ECO:0000313|Proteomes:UP000005667};
RX   PubMed=22216014; DOI=10.1371/journal.pgen.1002430;
RA   Wisniewski-Dye F., Borziak K., Khalsa-Moyers G., Alexandre G.,
RA   Sukharnikov L.O., Wuichet K., Hurst G.B., McDonald W.H., Robertson J.S.,
RA   Barbe V., Calteau A., Rouy Z., Mangenot S., Prigent-Combaret C.,
RA   Normand P., Boyer M., Siguier P., Dessaux Y., Elmerich C., Condemine G.,
RA   Krishnen G., Kennedy I., Paterson A.H., Gonzalez V., Mavingui P.,
RA   Zhulin I.B.;
RT   "Azospirillum genomes reveal transition of bacteria from aquatic to
RT   terrestrial environments.";
RL   PLoS Genet. 7:E1002430-E1002430(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC         cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC         Evidence={ECO:0000256|ARBA:ARBA00000966};
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DR   EMBL; FQ311869; CBS88800.1; -; Genomic_DNA.
DR   RefSeq; WP_014188254.1; NC_016585.1.
DR   AlphaFoldDB; G7ZAJ6; -.
DR   KEGG; ali:AZOLI_p10561; -.
DR   HOGENOM; CLU_355896_0_0_5; -.
DR   OrthoDB; 1153097at2; -.
DR   Proteomes; UP000005667; Plasmid AZO_p1.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0007154; P:cell communication; IEA:InterPro.
DR   Gene3D; 2.60.40.2030; -; 1.
DR   Gene3D; 2.60.60.40; -; 2.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR038081; CalX-like_sf.
DR   InterPro; IPR003644; Calx_beta.
DR   InterPro; IPR031768; CBM60_xylan-bd.
DR   InterPro; IPR001547; Glyco_hydro_5.
DR   InterPro; IPR018087; Glyco_hydro_5_CS.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR35923:SF2; CELLULASE DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR35923; MAJOR EXTRACELLULAR ENDOGLUCANASE; 1.
DR   Pfam; PF03160; Calx-beta; 1.
DR   Pfam; PF16841; CBM60; 2.
DR   Pfam; PF00150; Cellulase; 1.
DR   SMART; SM00237; Calx_beta; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF141072; CalX-like; 1.
DR   PROSITE; PS00659; GLYCOSYL_HYDROL_F5; 1.
PE   4: Predicted;
KW   Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000313|EMBL:CBS88800.1};
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:CBS88800.1};
KW   Plasmid {ECO:0000313|EMBL:CBS88800.1};
KW   Repeat {ECO:0000256|ARBA:ARBA00022737};
KW   Signal {ECO:0000256|ARBA:ARBA00022729}.
FT   DOMAIN          648..751
FT                   /note="Calx-beta"
FT                   /evidence="ECO:0000259|SMART:SM00237"
FT   REGION          276..312
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   839 AA;  88033 MW;  9C6AC92DF027C91F CRC64;
     MATNPVDMTF VVNASGVAAG GVWPHFKFLV DGVSVGQATV NSTSPGRYSF TTKVAPDAAH
     KVQVVYDNDW LSTDKTGMYR DLIVKSIEVN GKTIASNSST VSYAAEGQGT LSGREILSWS
     GSLDFNMSKD VFAGAAGTPT TPTPTPPATG GTPIVVNAYG NAAGGVNAHF KVLVDGKVIG
     EATAGTAAKD FTFNANVSAD MAHKVQIQFD NDGVVNGADR NLYVNKITIG GHAVAPTDSI
     VSIDKGALDG KDIVAGQKAM WWNSTMEVKA DQSWFAGATT PTPNPSNPSQ PSLSVSDVSV
     AEPTGNTSSG GIQGFLSTKG NQIVDEAGNA VRLTGINWFG GEGYNYVPAG LWADSYQGHL
     EKMKSVGFNT IRLTMSDEMF TSSAVTNGID YSKNPDLVGL TRLQVYDKVI EHAGKLGMKV
     ILDHHRNDGG AGTNEHGLWY SNRFPESTVI ENWKMLAKHY AGNETVIGAD LHNEPSVTAT
     WGGSAATDWA SAAERIGNAI QSVNKDWLLF VEGTEWSSTL AGAQSRPIEF DTPNKLVYSP
     HAYGHSVGNF SWLNDPAYPN NLPAQYDKMW GYLYKNDTAP ILLGEWGGLF NSSAEQTWAR
     TMGKYLNGDW NLDGKSDIAA GDKGMSWTFW AWTPESGDVG GILMDDYKTI NQSKINLIKP
     AMEGAVFDTP GGSSTPNTAN ATFTVKLSSA SGTPVTVDYA TVDGTAKAGS DYTATSGKLT
     FAAGETTKTV TVKVLSDSVT EGNETFGLKI SNSTAATIAD ATGTGTIVSA AAKAAAASLD
     SADLLAHTMT TANDPATAGD LSAAALASQD AFAADLSYAN LGVNYHELHT TDGSLAHAA
//