ID G7ZI97_AZOL4 Unreviewed; 443 AA.
AC G7ZI97;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 27-MAR-2024, entry version 70.
DE RecName: Full=Citrate synthase {ECO:0000256|PIRNR:PIRNR001369, ECO:0000256|RuleBase:RU003370};
GN Name=gltA2 {ECO:0000313|EMBL:CBS91232.1};
GN OrderedLocusNames=AZOLI_p50235 {ECO:0000313|EMBL:CBS91232.1};
OS Azospirillum lipoferum (strain 4B).
OG Plasmid AZO_p5 {ECO:0000313|EMBL:CBS91232.1,
OG ECO:0000313|Proteomes:UP000005667}.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Azospirillaceae; Azospirillum.
OX NCBI_TaxID=862719 {ECO:0000313|EMBL:CBS91232.1, ECO:0000313|Proteomes:UP000005667};
RN [1] {ECO:0000313|Proteomes:UP000005667}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=4B {ECO:0000313|Proteomes:UP000005667};
RC PLASMID=Plasmid AZO_p5 {ECO:0000313|Proteomes:UP000005667};
RX PubMed=22216014; DOI=10.1371/journal.pgen.1002430;
RA Wisniewski-Dye F., Borziak K., Khalsa-Moyers G., Alexandre G.,
RA Sukharnikov L.O., Wuichet K., Hurst G.B., McDonald W.H., Robertson J.S.,
RA Barbe V., Calteau A., Rouy Z., Mangenot S., Prigent-Combaret C.,
RA Normand P., Boyer M., Siguier P., Dessaux Y., Elmerich C., Condemine G.,
RA Krishnen G., Kennedy I., Paterson A.H., Gonzalez V., Mavingui P.,
RA Zhulin I.B.;
RT "Azospirillum genomes reveal transition of bacteria from aquatic to
RT terrestrial environments.";
RL PLoS Genet. 7:E1002430-E1002430(2011).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.16;
CC Evidence={ECO:0000256|ARBA:ARBA00000308,
CC ECO:0000256|RuleBase:RU003370};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 1/2. {ECO:0000256|ARBA:ARBA00004751,
CC ECO:0000256|RuleBase:RU003370}.
CC -!- SIMILARITY: Belongs to the citrate synthase family.
CC {ECO:0000256|ARBA:ARBA00010566, ECO:0000256|PIRNR:PIRNR001369,
CC ECO:0000256|RuleBase:RU003406}.
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DR EMBL; FQ311873; CBS91232.1; -; Genomic_DNA.
DR RefSeq; WP_014250054.1; NC_016624.1.
DR AlphaFoldDB; G7ZI97; -.
DR KEGG; ali:AZOLI_p50235; -.
DR HOGENOM; CLU_025068_0_0_5; -.
DR OrthoDB; 9800864at2; -.
DR UniPathway; UPA00223; UER00717.
DR Proteomes; UP000005667; Plasmid AZO_p5.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR CDD; cd06114; EcCS_like; 1.
DR Gene3D; 2.20.28.60; -; 1.
DR Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR InterPro; IPR002020; Citrate_synthase.
DR InterPro; IPR019810; Citrate_synthase_AS.
DR InterPro; IPR024176; Citrate_synthase_bac-typ.
DR InterPro; IPR036969; Citrate_synthase_sf.
DR InterPro; IPR010953; Citrate_synthase_typ-I.
DR NCBIfam; TIGR01798; cit_synth_I; 1.
DR PANTHER; PTHR42871; CITRATE SYNTHASE; 1.
DR PANTHER; PTHR42871:SF1; CITRATE SYNTHASE; 1.
DR Pfam; PF00285; Citrate_synt; 1.
DR PIRSF; PIRSF001369; Citrate_synth; 1.
DR PRINTS; PR00143; CITRTSNTHASE.
DR SUPFAM; SSF48256; Citrate synthase; 1.
DR PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000313|EMBL:CBS91232.1};
KW Plasmid {ECO:0000313|EMBL:CBS91232.1};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR001369};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532,
KW ECO:0000256|RuleBase:RU003370}.
FT ACT_SITE 320
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
FT ACT_SITE 378
FT /evidence="ECO:0000256|PIRSR:PIRSR001369-1"
SQ SEQUENCE 443 AA; 49546 MW; FD0CEA1CB21B65A9 CRC64;
MESSKHTPHG DVTQVGTVTL TDDATGKTLK LPLLGGSTGP NVIDIRKLYA ETGYFTYDPG
FTSTAACDSA ITYIDGDEGV LLHRGYAIAD LAENCDFLEV CHLLLNGELP NPQQKADFER
TITYHTMVHE QLAKFYSGFR RDAHPMAIMC GVVGALSAFY HDSTDIFDPV QRKIAAHRLI
AKMPTMAAMA HKYTVGEPFM YPRNDLTYAE NFLYMTFGTP CEEWTVDPVL SEAMDKIFIL
HADHEQNAST STVRLAGSSH ANPFACIASG IAALWGPAHG GANEAVLKML EEIGSVDRIP
EFVRRAKDKN DSFRLMGFGH RVYKNYDPRA KIMRETCYQV LDRLGIHDEP HLAIAMELER
IALSDEYFIE KKLYPNVDFY SGIILKAMGF PTSMFTVLFA LARTVGWISQ WKEMIEDPAQ
KIGRPRQLFT GHAARPFVPL DKR
//
