ID G8BRF9_TETPH Unreviewed; 568 AA.
AC G8BRF9;
DT 25-JAN-2012, integrated into UniProtKB/TrEMBL.
DT 25-JAN-2012, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=tRNA (uracil-O(2)-)-methyltransferase {ECO:0000256|ARBA:ARBA00017788, ECO:0000256|RuleBase:RU368004};
DE EC=2.1.1.211 {ECO:0000256|ARBA:ARBA00012795, ECO:0000256|RuleBase:RU368004};
GN Name=TPHA0C01790 {ECO:0000313|EMBL:CCE62335.1};
GN OrderedLocusNames=TPHA_0C01790 {ECO:0000313|EMBL:CCE62335.1};
OS Tetrapisispora phaffii (strain ATCC 24235 / CBS 4417 / NBRC 1672 / NRRL
OS Y-8282 / UCD 70-5) (Yeast) (Fabospora phaffii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Tetrapisispora.
OX NCBI_TaxID=1071381 {ECO:0000313|EMBL:CCE62335.1, ECO:0000313|Proteomes:UP000005666};
RN [1] {ECO:0000313|EMBL:CCE62335.1, ECO:0000313|Proteomes:UP000005666}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24235 / CBS 4417 / NBRC 1672 / NRRL Y-8282 / UCD 70-5
RC {ECO:0000313|Proteomes:UP000005666};
RX PubMed=22123960; DOI=10.1073/pnas.1112808108;
RA Gordon J.L., Armisen D., Proux-Wera E., OhEigeartaigh S.S., Byrne K.P.,
RA Wolfe K.H.;
RT "Evolutionary erosion of yeast sex chromosomes by mating-type switching
RT accidents.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:20024-20029(2011).
CC -!- FUNCTION: Adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-
CC methyltransferase. {ECO:0000256|RuleBase:RU368004}.
CC -!- FUNCTION: Probable adenosyl-L-methionine (AdoMet)-dependent tRNA
CC (uracil-O(2)-)-methyltransferase. {ECO:0000256|ARBA:ARBA00002778}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC Evidence={ECO:0000256|ARBA:ARBA00000660,
CC ECO:0000256|RuleBase:RU368004};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|RuleBase:RU368004}.
CC -!- SIMILARITY: Belongs to the TRM44 family.
CC {ECO:0000256|ARBA:ARBA00009056, ECO:0000256|RuleBase:RU368004}.
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DR EMBL; HE612858; CCE62335.1; -; Genomic_DNA.
DR RefSeq; XP_003684769.1; XM_003684721.1.
DR AlphaFoldDB; G8BRF9; -.
DR STRING; 1071381.G8BRF9; -.
DR GeneID; 11533940; -.
DR KEGG; tpf:TPHA_0C01790; -.
DR eggNOG; KOG3790; Eukaryota.
DR HOGENOM; CLU_018580_2_0_1; -.
DR OMA; IREPNIN; -.
DR OrthoDB; 1353816at2759; -.
DR Proteomes; UP000005666; Chromosome 3.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0141101; F:tRNA(Ser) (uridine(44)-2'-O-)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR PANTHER; PTHR21210:SF0; TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED; 1.
DR PANTHER; PTHR21210; UNCHARACTERIZED; 1.
DR Pfam; PF07757; AdoMet_MTase; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|RuleBase:RU368004};
KW Methyltransferase {ECO:0000256|RuleBase:RU368004};
KW Reference proteome {ECO:0000313|Proteomes:UP000005666};
KW S-adenosyl-L-methionine {ECO:0000256|RuleBase:RU368004};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU368004};
KW tRNA processing {ECO:0000256|RuleBase:RU368004}.
FT REGION 467..486
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 568 AA; 65873 MW; A820ABB309FB9544 CRC64;
MTESKSKSQR DQFITKINEP SILGSEWYCM YETDTKVNFL REHFETAMMN VIREPNINST
AVLRADILKE IKYENSKTDD KIDHNEEITI DNTNNEDFNI SVADSKQTNI LDMELRIPQL
SEELDFHTFL GIVRRMVPRN PYKDALINQT CLMMESPVRY PESSLVIYTP HLDKKDDCPF
YIPAVKNVAI LFHEEHLSVH YLFFSDEQMK QLIQDESERI VRTAWRLLQT AYKHSRGVMQ
GYEKRVNHDQ VVDKVKFQNQ YITIKKKYSQ FLVENWKEST DPKKHVFEDI AIAAFLLELW
NKIYGPDFKN KMQFRDLGCG NGVLCYILIE EGCKGIGIDA RHRKSWAMYP TTVRKCLKEQ
VIVPSVLLRP HPDLKKNNPY LTHNNNLFPV KMTSQDLIAP ATVLFTSEDL LNSPQVNATE
FPTDTFVIGN HSDELTCWIP LLGYNFMIIP CCSHNFSGQK IRFNVRKQPK PDKQASSDKN
ASSSKISNST YAGLVDHVCY ISEKVGWKIE KEMLRIPSTR NAAIIGYKND KLSEFPTQEV
YDMLQEDGGP SGWIHNTMQL MKKNPRNH
//
