ID G8R3P7_OWEHD Unreviewed; 1430 AA.
AC G8R3P7;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE RecName: Full=DNA polymerase III subunit alpha {ECO:0000256|ARBA:ARBA00019114};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN OrderedLocusNames=Oweho_3182 {ECO:0000313|EMBL:AEV34134.1};
OS Owenweeksia hongkongensis (strain DSM 17368 / CIP 108786 / JCM 12287 / NRRL
OS B-23963 / UST20020801).
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales; Owenweeksiaceae;
OC Owenweeksia.
OX NCBI_TaxID=926562 {ECO:0000313|EMBL:AEV34134.1, ECO:0000313|Proteomes:UP000005631};
RN [1] {ECO:0000313|EMBL:AEV34134.1, ECO:0000313|Proteomes:UP000005631}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=DSM 17368 / JCM 12287 / NRRL B-23963
RC {ECO:0000313|Proteomes:UP000005631};
RX PubMed=23450211; DOI=10.4056/sigs.3296896;
RA Riedel T., Held B., Nolan M., Lucas S., Lapidus A., Tice H., Del Rio T.G.,
RA Cheng J.F., Han C., Tapia R., Goodwin L.A., Pitluck S., Liolios K.,
RA Mavromatis K., Pagani I., Ivanova N., Mikhailova N., Pati A., Chen A.,
RA Palaniappan K., Rohde M., Tindall B.J., Detter J.C., Goker M., Woyke T.,
RA Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Klenk H.P.,
RA Kyrpides N.C.;
RT "Genome sequence of the orange-pigmented seawater bacterium Owenweeksia
RT hongkongensis type strain (UST20020801(T)).";
RL Stand. Genomic Sci. 7:120-130(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR EMBL; CP003156; AEV34134.1; -; Genomic_DNA.
DR RefSeq; WP_014203481.1; NC_016599.1.
DR STRING; 926562.Oweho_3182; -.
DR KEGG; oho:Oweho_3182; -.
DR PATRIC; fig|926562.3.peg.3204; -.
DR eggNOG; COG0587; Bacteria.
DR HOGENOM; CLU_001600_1_0_10; -.
DR OrthoDB; 9803237at2; -.
DR Proteomes; UP000005631; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd06127; DEDDh; 1.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd12113; PHP_PolIIIA_DnaE3; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 1.10.10.1600; Bacterial DNA polymerase III alpha subunit, thumb domain; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR041931; DNA_pol3_alpha_thumb_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF0; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF00929; RNase_T; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00479; EXOIII; 1.
DR SMART; SM00481; POLIIIAc; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 4: Predicted;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000313|EMBL:AEV34134.1};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000005631};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1..191
FT /note="Exonuclease"
FT /evidence="ECO:0000259|SMART:SM00479"
FT DOMAIN 254..321
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
SQ SEQUENCE 1430 AA; 161198 MW; 9D8B130FDCBE02C8 CRC64;
MFLIFDTETT GLPLRDDAPL TDFENWPRAV QIAWQLHDDD GSLIEVKDYV IRPDGFDIPF
NAAKIHGITT EVAEHYGVPL EQAMAEFSEA VKKSKYLVGH NIQFDINVLG CEYLRLEQDN
LFEGMGSIDT GEETKNYCKL PGGRGGGFKM PKLEELHTIL FGEGFAEAHN AAADVEATSR
AFLEAVRIGI YTDERLDWKP ENREAFNRVN PDTVQPIGLK VESFKELARK LAEKSASDRQ
NEEKIELSAD TSFVHLHVHT QFSILQATCD IPGLVNKAKS LGMPAVAMTD HANLYGAFHF
VQTALANDIK PIVGCEFYVN RNHKDKTVKD NGFQVLFLAK NKAGYHNLAK LSSCAFVDGF
YYVPRIDKEL ILQYKDDLIV TTGNTYGEVP SLILNVGEKQ AEEAFVWYHE NFGEDFYVEL
LDHGLDEEKV VNETLLKFAR KYGVKYFGSN NVYYLGKEDA NAHDILLCVK EGEYQSTPKG
RGRGFRFGFP NDEFYFKDTE AMKDIFKDTP EAILTTLEIA DKIESYTLAR DVLLPAFDIP
ERFIDPADEE DGGKRGENAY LRHLTYVGAE KRYGEITEEI KQRLDFELET IANTGYPGYF
LIVQDFTTEA RNMGVSVGPG RGSAAGSAVA YCIGITNVDP IAYDLLFERF LNPDRVSLPD
IDIDFDDEGR PRIIDWVVKK YGSNQVAQII TYGSMAAKSA IRDTGRVLQL PLSETDKIAK
LVPDTKLSKL FSWSDKEMSQ NLNGDLYQLG KQLRDLAETN GPEAQVINQA RILEGSVRNT
GIHACGVIIT PDDITKFVPV SLAKDSDLYV TQFDNSVVES AGMLKMDFLG LKTLTIIKDA
IAIIKQRHDV HIDPDEIPLD DEKTFELYQR GETNGTFQFE SAGMQKHLKA LKPDKFADLI
AMNALYRPGP IEYIPNFIAR KNGQEEIAYD LPEMEEYLAE TYGITVYQEQ VMLLSQSLAG
FTKGEADVLR KAMGKKQKAV LDKMKPKFIE QGSERGHDAT ILEKVWKDWE AFAAYAFNKS
HSTCYSVVAF HTGYLKANYP AEYMASVLTH NMSDIKKVTF FMEECKRMGI PVLGPDVNES
QFKFAVNAKG EIRFGLGGMK GVGENAVMAI VNERQENGPY QNIFDMLKRI DLRAVNKKTL
ENLALGGAFD LYEGSNRAVY FYANGDEKTF LERAIKYAQN YKAAEDSSQV SLFGGSAEVD
LPEPEIPQVE EWPRLMRLNK EKDVNGIYLS SHPLDDFKFE MKFLCTHELT ALNTPAGIAD
YTVAGIITEA RHLMSKQGKP FGFFTIEDYS GNYEYRLFGD EYLKVKPFME SNMMVMISGY
VKRYTPWQGA EERIEAKIKE IKLLPDVMES ESKSVAIKIP LPNVTPQNID DLNDLVDRYR
GNKRLSLLIF DPEKPKVNVR MPSRGGGILI NKDLLDELSS RDYMKIALNK
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