ID   G8SBN5_ACTS5            Unreviewed;      2088 AA.
AC   G8SBN5;
DT   22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT   22-FEB-2012, sequence version 1.
DT   27-MAR-2024, entry version 66.
DE   SubName: Full=Non-ribosomal peptide synthetase {ECO:0000313|EMBL:AEV84014.1};
DE            EC=5.1.1.13 {ECO:0000313|EMBL:AEV84014.1};
GN   Name=lgrB {ECO:0000313|EMBL:AEV84014.1};
GN   OrderedLocusNames=ACPL_3119 {ECO:0000313|EMBL:AEV84014.1};
OS   Actinoplanes sp. (strain ATCC 31044 / CBS 674.73 / SE50/110).
OC   Bacteria; Actinomycetota; Actinomycetes; Micromonosporales;
OC   Micromonosporaceae; Actinoplanes.
OX   NCBI_TaxID=134676 {ECO:0000313|EMBL:AEV84014.1, ECO:0000313|Proteomes:UP000005440};
RN   [1] {ECO:0000313|Proteomes:UP000005440}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 31044 / CBS 674.73 / SE50/110
RC   {ECO:0000313|Proteomes:UP000005440};
RA   Schwientek P., Szczepanowski R., Kalinowski J., Klein A., Selber K.,
RA   Wehmeier U.F., Stoye J., Puehler A.;
RT   "The complete genome sequence of the acarbose producer Actinoplanes sp.
RT   SE50/110.";
RL   Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC         Evidence={ECO:0000256|ARBA:ARBA00001957};
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DR   EMBL; CP003170; AEV84014.1; -; Genomic_DNA.
DR   RefSeq; WP_014690086.1; NZ_LT827010.1.
DR   STRING; 134676.ACPL_3119; -.
DR   KEGG; ase:ACPL_3119; -.
DR   PATRIC; fig|134676.3.peg.3040; -.
DR   eggNOG; COG1020; Bacteria.
DR   HOGENOM; CLU_000022_0_3_11; -.
DR   OrthoDB; 5476914at2; -.
DR   Proteomes; UP000005440; Chromosome.
DR   GO; GO:0047689; F:aspartate racemase activity; IEA:UniProtKB-EC.
DR   GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR   GO; GO:0043604; P:amide biosynthetic process; IEA:UniProt.
DR   GO; GO:0008610; P:lipid biosynthetic process; IEA:UniProt.
DR   GO; GO:1901566; P:organonitrogen compound biosynthetic process; IEA:UniProt.
DR   CDD; cd17652; A_NRPS_CmdD_like; 2.
DR   CDD; cd19540; LCL_NRPS-like; 1.
DR   Gene3D; 3.30.300.30; -; 2.
DR   Gene3D; 3.40.50.980; -; 4.
DR   Gene3D; 1.10.1200.10; ACP-like; 1.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 2.
DR   Gene3D; 3.30.559.30; Nonribosomal peptide synthetase, condensation domain; 2.
DR   InterPro; IPR010071; AA_adenyl_domain.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR025110; AMP-bd_C.
DR   InterPro; IPR045851; AMP-bd_C_sf.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR   InterPro; IPR023213; CAT-like_dom_sf.
DR   InterPro; IPR001242; Condensatn.
DR   InterPro; IPR020806; PKS_PP-bd.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   InterPro; IPR006162; Ppantetheine_attach_site.
DR   NCBIfam; TIGR01733; AA-adenyl-dom; 2.
DR   PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR   PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR   Pfam; PF00501; AMP-binding; 2.
DR   Pfam; PF13193; AMP-binding_C; 2.
DR   Pfam; PF00668; Condensation; 2.
DR   Pfam; PF00550; PP-binding; 2.
DR   SMART; SM00823; PKS_PP; 2.
DR   SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 2.
DR   SUPFAM; SSF47336; ACP-like; 2.
DR   SUPFAM; SSF52777; CoA-dependent acyltransferases; 4.
DR   PROSITE; PS00455; AMP_BINDING; 2.
DR   PROSITE; PS50075; CARRIER; 2.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 2.
PE   4: Predicted;
KW   Isomerase {ECO:0000313|EMBL:AEV84014.1};
KW   Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW   Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005440}.
FT   DOMAIN          947..1022
FT                   /note="Carrier"
FT                   /evidence="ECO:0000259|PROSITE:PS50075"
FT   DOMAIN          1997..2072
FT                   /note="Carrier"
FT                   /evidence="ECO:0000259|PROSITE:PS50075"
FT   REGION          926..947
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   2088 AA;  223019 MW;  59155D25FC745E0B CRC64;
     MTIPLTAAQR GIWYARQLAS DSRMHTIGEY LDITGPIRTD VFEAALRRTV DEADCLTVRF
     TATPDGEVHQ QTGAAAPWRL TVDDLSDEAD PFEAAMRWMR TDLDRPFEPE AGRVFRYALL
     RLSPGRHLWW SSYHHLVLDG FGMGLITRRV AEVYTALHAG TAVPACPFGR LSDLVARDRA
     YQESPRRTQD QQFWADYLRD CPQPRGLAHA QPAPAGPVLR RSGWIDGATA RLLTERAEAC
     GTRWPLVVVA AWAAYLHRMT GEREVVVGLP AAARRDPHDR VTPAMVSNLL PLRVRVDSGV
     GVTALVEQVA AQARIVLAHQ NYRVEDLRRD RSPGAELIAS RANVMSFAYG VSFGDARARA
     YNLAGGFPED LAMLVWDRRD GEDYRVDFDA NPRRYSAPDV VGHRDRFLAF LTRFATADPP
     VEPARLDLLT DADRAATARW AGPGPAIEAA TLAELFAAQV TRTPQAPALV GDDTTLTYAQ
     LDAVTDRLAR SLLARGVGAE DLVALAFPRS VHWVTAMLAV TKTGAAYLPI DPAYPADRIR
     YMLTDARPVL LLSSAETAAT VPCAGVPVMI LDSADPADPA APPARPAPAR PAGADNTAYV
     IYTSGSTGRP KGVAVTHRGL AGLAAAQGER LCVRPGDRML QFASPSFDAA QSDIWVTLLA
     GATLVTPSAD RLTVGPPLAA TVAERGVTHL KLPPAALAVL PGDALDGIRV LAVAGEALPE
     SLVARWHTGR RMINVYGPTE NTVTATLSGP LTPGGPVPIG TPITGTRAYV LDTRLRPVPP
     NAVGELYLTG AGLARGYLRR PALTAGRFVA CPFGAPGTRM YRTGDLARWT SDGRLVFAGR
     ADDQVKLRGF RIELGEIEAV LSEHPGVRQA VATVREDRGG DRRLVAYVVG DAAPADVRAR
     AAATLPAHMV PAAVVVVDRI PLTPNGKLDR QSLPAPDWSA AGEPARTPRD DREELLCRLF
     AQLLGVPSVG PGDDFFALGG HSLLATRLLG RIRTVLRAEV SLRELFAAPT PARLAELVAG
     SRRGPRPPLR PVPRPEAPPL SAAQRRLWFL HRLQGPGATY NMPYALRLTG PLDVAALRAA
     LTDVVGRHES LRTVFAAPDG VPHQVILPAD RATPEISRPA GDPGQWLSRA AHLPFDLSRD
     LPIRVHLGVL GPDEHLLLMV LNHVAADGWS LAPLMRDLST AYAARLGGTG PAWPPLPVQY
     TDYALWQSRL LGTIDDPDSL LSRQLAFWRT ALAGAPQHLE LPTDRPYPVE RSGVGGQVPF
     TVPAQLHRDL LRLAHDHDAT LFMVLHAAFA VLLHRVGAGD DIVIGTPVAG RADEELDDLV
     GFFVNTLALR TDLSGDPGFG ALLDRVRDHD LDAFAHQDLP FDRLVEALVP DRSTARQPLV
     QVMVTLQRAA DVALSLPGVH AEAHPVHLDV AKFDLTATIV EHVGAAGQPA GLDGRLEYAT
     DVLDRRSGQA LAERLLRLLR QVAAEPEQRI GRLDVLDPAE RQRMLVDGNA TATAVPDGTL
     AALFAAQVAR TPDATAVIGD DVTLTYAALD ARANRLARWL IAAGIGPEDI VALSFPRGVR
     WVTAILAVAK AGAAFLPIDP TYPAARIRYM VEDARPRMVL TVTATEGGLP ALAAPVTALD
     GEEIATALPA LPDTLPPCPA TPANTLYVIY TSGSTGRPKG VAVTHTGIAS LAVAKAARFG
     LDGTSRVLQF ASPSFDAAVW EICPALLSGA ALVVPSADRL TVGAPLAATI AEAGVTHLTL
     PPAALAVLPG DALAGVRTLG VAGESPAAEV VQRWSPGRTM VNAYGPTETT VCATVSRPLT
     PRDRPAPIGG PITGTRVYVL DARLVPVPPG VPGELYVAGA GLARGYLRRP AMTAERFVAC
     PWGAAGERMY RTGDLVKWTG DGQLLFLGRT DDQVKIRGFR IELGEITGVV AGQSGVRQAI
     VVAREDRPGE KTLAAYVTEA AGRTLDPQTL RDKLAAELPG HMVPAVVVLD AMPLTANGKI
     DKAALPAPNT VAAPGRAPRD AREEVLCALF AELLGVPEVG IDDDFFQLGG HSLLATRLIS
     RIRAALRADL TIRDLFTART PARIGERLTT ARPARPALRR MAKKEDAS
//