ID G8Y940_PICSO Unreviewed; 549 AA.
AC G8Y940;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 58.
DE SubName: Full=Piso0_004551 protein {ECO:0000313|EMBL:CCE83954.1};
GN Name=Piso0_004551 {ECO:0000313|EMBL:CCE83954.1};
GN ORFNames=GNLVRS01_PISO0K19370g {ECO:0000313|EMBL:CCE83954.1},
GN GNLVRS01_PISO0L19371g {ECO:0000313|EMBL:CCE84985.1};
OS Pichia sorbitophila (strain ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC
OS 10061 / NRRL Y-12695) (Hybrid yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Millerozyma.
OX NCBI_TaxID=559304 {ECO:0000313|EMBL:CCE83954.1, ECO:0000313|Proteomes:UP000005222};
RN [1] {ECO:0000313|EMBL:CCE83954.1}
RP NUCLEOTIDE SEQUENCE.
RA Genoscope - CEA;
RL Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000005222}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC 10061 / NRRL
RC Y-12695 {ECO:0000313|Proteomes:UP000005222};
RX DOI=10.1534/g3.111.000745;
RA Leh Louis V., Despons L., Friedrich A., Martin T., Durrens P.,
RA Casaregola S., Neuveglise C., Fairhead C., Marck C., Cruz J.A.,
RA Straub M.L., Kugler V., Sacerdot C., Uzunov Z., Thierry A., Weiss S.,
RA Bleykasten C., De Montigny J., Jacques N., Jung P., Lemaire M., Mallet S.,
RA Morel G., Richard G.F., Sarkar A., Savel G., Schacherer J., Seret M.L.,
RA Talla E., Samson G., Jubin C., Poulain J., Vacherie B., Barbe V.,
RA Pelletier E., Sherman D.J., Westhof E., Weissenbach J., Baret P.V.,
RA Wincker P., Gaillardin C., Dujon B., Souciet J.L.;
RT "Pichia sorbitophila, an interspecies yeast hybrid reveals early steps of
RT genome resolution following polyploidization.";
RL G3 (Bethesda) 2:299-311(2012).
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000256|ARBA:ARBA00009375}.
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DR EMBL; FO082049; CCE83954.1; -; Genomic_DNA.
DR EMBL; FO082048; CCE84985.1; -; Genomic_DNA.
DR AlphaFoldDB; G8Y940; -.
DR STRING; 559304.G8Y940; -.
DR eggNOG; KOG2553; Eukaryota.
DR HOGENOM; CLU_021971_0_1_1; -.
DR InParanoid; G8Y940; -.
DR OMA; WNIDIIV; -.
DR Proteomes; UP000005222; Chromosome K.
DR Proteomes; UP000005222; Chromosome L.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IEA:UniProt.
DR GO; GO:0009982; F:pseudouridine synthase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0031119; P:tRNA pseudouridine synthesis; IEA:InterPro.
DR CDD; cd02568; PseudoU_synth_PUS1_PUS2; 1.
DR Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR041708; PUS1/PUS2-like.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR NCBIfam; TIGR00071; hisT_truA; 1.
DR PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR11142:SF4; PSEUDOURIDYLATE SYNTHASE 1 HOMOLOG; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Reference proteome {ECO:0000313|Proteomes:UP000005222};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694}.
FT DOMAIN 315..421
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
FT REGION 253..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 505..549
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 533..549
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 130
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-1"
FT BINDING 186
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR641708-2"
SQ SEQUENCE 549 AA; 62152 MW; 350285A9D1A00555 CRC64;
MMLSFIKGLN KINGLVPARE IRAREVAKGL RRKHKSVDRE TTMMRRTEYK PRLDENGNPI
PKSPRRPKKK VACMIGYCGT GYNGMQIQNN PNIETIEGDL FKAFVAAGAI SPENADDLKK
NGFMRAARTD KGVHAAGNVI SCKLIIEDED IIEKINAQLP DQIRVWGIER TNKSFDCRKM
CSSRVYEYLL PTYSLLPPKP RSVLAELLRK EKENYPEIMR KDEEGEKWWE ETLQAVFDSG
ISPDDLAKAQ KILESSGSSG SNKAEEEGKE KTKLYDDDGN ITEAGKLIKS IKSVENKRRR
AYNISTERLN IFREALNQYE GAHNFHNFTL GKTFKDPSSV RYMKKLTVSD PFVIEGTEWV
SIKIHGQSFM LHQIRKMIAM AALVIRTGCP LERISDCFGP IKVNIPKAPA LGLLLENPVY
DGYNTTLEKH GYNAIDFSKY QEEMDKFKMK YIYDKIYAEE VKENTFYGFF GFVDSFKGTS
DTKEGRHVFD FLGAKFDSAA LKKHDKVEEA AEAAEPTEPV EPSQTEPSGS DAVSNTEEKD
DDSSTTKDN
//
