ID G8YBU1_PICSO Unreviewed; 408 AA.
AC G8YBU1;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 18-JUN-2025, entry version 54.
DE RecName: Full=Ribosomal RNA-processing protein 8 {ECO:0000256|ARBA:ARBA00076672, ECO:0000256|RuleBase:RU365074};
DE EC=2.1.1.- {ECO:0000256|RuleBase:RU365074};
GN Name=Piso0_002148 {ECO:0000313|EMBL:CCE82422.1};
GN ORFNames=GNLVRS01_PISO0J05707g {ECO:0000313|EMBL:CCE82422.1};
OS Pichia sorbitophila (strain ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC
OS 10061 / NRRL Y-12695) (Hybrid yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Pichiomycetes;
OC Debaryomycetaceae; Millerozyma.
OX NCBI_TaxID=559304 {ECO:0000313|EMBL:CCE82422.1, ECO:0000313|Proteomes:UP000005222};
RN [1] {ECO:0000313|Proteomes:UP000005222}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC 10061 / NRRL
RC Y-12695 {ECO:0000313|Proteomes:UP000005222};
RX DOI=10.1534/g3.111.000745;
RA Leh Louis V., Despons L., Friedrich A., Martin T., Durrens P.,
RA Casaregola S., Neuveglise C., Fairhead C., Marck C., Cruz J.A.,
RA Straub M.L., Kugler V., Sacerdot C., Uzunov Z., Thierry A., Weiss S.,
RA Bleykasten C., De Montigny J., Jacques N., Jung P., Lemaire M., Mallet S.,
RA Morel G., Richard G.F., Sarkar A., Savel G., Schacherer J., Seret M.L.,
RA Talla E., Samson G., Jubin C., Poulain J., Vacherie B., Barbe V.,
RA Pelletier E., Sherman D.J., Westhof E., Weissenbach J., Baret P.V.,
RA Wincker P., Gaillardin C., Dujon B., Souciet J.L.;
RT "Pichia sorbitophila, an interspecies yeast hybrid reveals early steps of
RT genome resolution following polyploidization.";
RL G3 (Bethesda) 2:299-311(2012).
CC -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that
CC specifically methylates the N(1) position of adenine in helix 25.1 in
CC 25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis.
CC Required for efficient pre-rRNA cleavage at site A2.
CC {ECO:0000256|RuleBase:RU365074}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000256|ARBA:ARBA00004604, ECO:0000256|RuleBase:RU365074}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. RRP8 family.
CC {ECO:0000256|ARBA:ARBA00006301, ECO:0000256|RuleBase:RU365074}.
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DR EMBL; FO082050; CCE82422.1; -; Genomic_DNA.
DR AlphaFoldDB; G8YBU1; -.
DR FunCoup; G8YBU1; 500.
DR STRING; 559304.G8YBU1; -.
DR eggNOG; KOG3045; Eukaryota.
DR HOGENOM; CLU_027694_1_0_1; -.
DR InParanoid; G8YBU1; -.
DR OMA; SCTIVVF; -.
DR OrthoDB; 10258825at2759; -.
DR Proteomes; UP000005222; Chromosome J.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0016433; F:rRNA (adenine) methyltransferase activity; IEA:TreeGrafter.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; IEA:TreeGrafter.
DR CDD; cd02440; AdoMet_MTases; 1.
DR FunFam; 1.10.10.2150:FF:000001; Ribosomal RNA-processing protein 8; 1.
DR FunFam; 3.40.50.150:FF:000320; Ribosomal RNA-processing protein 8; 1.
DR Gene3D; 1.10.10.2150; Ribosomal RNA-processing protein 8, N-terminal domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR007823; RRP8.
DR InterPro; IPR042036; RRP8_N.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR12787; RIBOSOMAL RNA-PROCESSING PROTEIN 8; 1.
DR PANTHER; PTHR12787:SF0; RIBOSOMAL RNA-PROCESSING PROTEIN 8; 1.
DR Pfam; PF05148; Methyltransf_8; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000256|RuleBase:RU365074};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242, ECO:0000256|RuleBase:RU365074};
KW Reference proteome {ECO:0000313|Proteomes:UP000005222};
KW rRNA processing {ECO:0000256|ARBA:ARBA00022552,
KW ECO:0000256|RuleBase:RU365074};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|RuleBase:RU365074};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365074}.
FT REGION 1..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 360..387
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 23..33
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 58..97
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 408 AA; 47074 MW; 2068BA5883E1D584 CRC64;
MALFEVEGWN IKADDVAVGG IPKNKKQKSK EKKAKRDEMS SRKRKHDEKE QEEDVEEKQE
SKESSRKAQK SESTKGKKMG GNEKKETTER KKPETRTEQS PLPITQNLTP LQQKMMAKLS
GSRFRWINEQ LYTVSSEDAF SLIQEQPSLF DEYHQGFRAQ VQSWPENPVD VFVDQIKQRL
STRPVNAPGG MPGLPNKDVM IADMGCGEAQ LALDVNNFTK EFNSAKKGKK KNRNGPQNSV
NVKVHSFDLK QTNERITVAD IKNVPLPDES CSVVIFCLAL MGTNFLDFVE EAYRILAPRG
ELWVAEIKSR FAESSDNKVL RPEDVGSEFV EALKLCGFFH KKTDNSNKMF SRFEFFKPPK
EILEERKAKL ERRKKFIEQE SEKEKLHQKR TEHPEGEWLL KPCIYKRR
//
