ID   G8YBU1_PICSO            Unreviewed;       408 AA.
AC   G8YBU1;
DT   22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT   22-FEB-2012, sequence version 1.
DT   11-DEC-2019, entry version 35.
DE   RecName: Full=Ribosomal RNA-processing protein 8 {ECO:0000256|RuleBase:RU365074};
DE            EC=2.1.1.- {ECO:0000256|RuleBase:RU365074};
GN   Name=Piso0_002148 {ECO:0000313|EMBL:CCE82422.1};
GN   ORFNames=GNLVRS01_PISO0J05707g {ECO:0000313|EMBL:CCE82422.1};
OS   Pichia sorbitophila (strain ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC
OS   10061 / NRRL Y-12695) (Hybrid yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Millerozyma.
OX   NCBI_TaxID=559304 {ECO:0000313|EMBL:CCE82422.1, ECO:0000313|Proteomes:UP000005222};
RN   [1] {ECO:0000313|Proteomes:UP000005222}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC 10061 / NRRL
RC   Y-12695 {ECO:0000313|Proteomes:UP000005222};
RX   DOI=10.1534/g3.111.000745;
RA   Leh Louis V., Despons L., Friedrich A., Martin T., Durrens P.,
RA   Casaregola S., Neuveglise C., Fairhead C., Marck C., Cruz J.A.,
RA   Straub M.L., Kugler V., Sacerdot C., Uzunov Z., Thierry A., Weiss S.,
RA   Bleykasten C., De Montigny J., Jacques N., Jung P., Lemaire M., Mallet S.,
RA   Morel G., Richard G.F., Sarkar A., Savel G., Schacherer J., Seret M.L.,
RA   Talla E., Samson G., Jubin C., Poulain J., Vacherie B., Barbe V.,
RA   Pelletier E., Sherman D.J., Westhof E., Weissenbach J., Baret P.V.,
RA   Wincker P., Gaillardin C., Dujon B., Souciet J.L.;
RT   "Pichia sorbitophila, an interspecies yeast hybrid reveals early steps of
RT   genome resolution following polyploidization.";
RL   G3 (Bethesda) 2:299-311(2012).
CC   -!- FUNCTION: S-adenosyl-L-methionine-dependent methyltransferase that
CC       specifically methylates the N(1) position of adenine in helix 25.1 in
CC       25S rRNA. Required both for ribosomal 40S and 60S subunits biogenesis.
CC       Required for efficient pre-rRNA cleavage at site A2.
CC       {ECO:0000256|RuleBase:RU365074}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC       {ECO:0000256|RuleBase:RU365074}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. RRP8 family.
CC       {ECO:0000256|RuleBase:RU365074}.
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DR   EMBL; FO082050; CCE82422.1; -; Genomic_DNA.
DR   STRING; 4920.XP_004198759.1; -.
DR   EnsemblFungi; CCE82422; CCE82422; GNLVRS01_PISO0J05707g.
DR   OMA; FHKKTDD; -.
DR   Proteomes; UP000005222; Chromosome J.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.10.2150; -; 1.
DR   InterPro; IPR007823; RRP8.
DR   InterPro; IPR042036; RRP8_N.
DR   InterPro; IPR029063; SAM-dependent_MTases.
DR   PANTHER; PTHR12787; PTHR12787; 1.
DR   Pfam; PF05148; Methyltransf_8; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   3: Inferred from homology;
KW   Coiled coil {ECO:0000256|SAM:Coils};
KW   Methyltransferase {ECO:0000256|RuleBase:RU365074};
KW   Nucleus {ECO:0000256|RuleBase:RU365074};
KW   Reference proteome {ECO:0000313|Proteomes:UP000005222};
KW   rRNA processing {ECO:0000256|RuleBase:RU365074};
KW   S-adenosyl-L-methionine {ECO:0000256|RuleBase:RU365074};
KW   Transferase {ECO:0000256|RuleBase:RU365074}.
FT   REGION          1..103
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          360..387
FT                   /evidence="ECO:0000256|SAM:Coils"
FT   COMPBIAS        19..97
FT                   /note="Polyampholyte"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   408 AA;  47074 MW;  2068BA5883E1D584 CRC64;
     MALFEVEGWN IKADDVAVGG IPKNKKQKSK EKKAKRDEMS SRKRKHDEKE QEEDVEEKQE
     SKESSRKAQK SESTKGKKMG GNEKKETTER KKPETRTEQS PLPITQNLTP LQQKMMAKLS
     GSRFRWINEQ LYTVSSEDAF SLIQEQPSLF DEYHQGFRAQ VQSWPENPVD VFVDQIKQRL
     STRPVNAPGG MPGLPNKDVM IADMGCGEAQ LALDVNNFTK EFNSAKKGKK KNRNGPQNSV
     NVKVHSFDLK QTNERITVAD IKNVPLPDES CSVVIFCLAL MGTNFLDFVE EAYRILAPRG
     ELWVAEIKSR FAESSDNKVL RPEDVGSEFV EALKLCGFFH KKTDNSNKMF SRFEFFKPPK
     EILEERKAKL ERRKKFIEQE SEKEKLHQKR TEHPEGEWLL KPCIYKRR
//