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Database: UniProt
Entry: GABPA_HUMAN
LinkDB: GABPA_HUMAN
Original site: GABPA_HUMAN 
ID   GABPA_HUMAN             Reviewed;         454 AA.
AC   Q06546; Q12939;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   16-OCT-2019, entry version 192.
DE   RecName: Full=GA-binding protein alpha chain;
DE            Short=GABP subunit alpha;
DE   AltName: Full=Nuclear respiratory factor 2 subunit alpha;
DE   AltName: Full=Transcription factor E4TF1-60;
GN   Name=GABPA; Synonyms=E4TF1A;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8441384; DOI=10.1128/mcb.13.3.1385;
RA   Watanabe H., Sawada J., Yano K., Yamaguchi K., Goto M., Handa H.;
RT   "cDNA cloning of transcription factor E4TF1 subunits with Ets and
RT   notch motifs.";
RL   Mol. Cell. Biol. 13:1385-1391(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=7799916; DOI=10.1128/mcb.15.1.102;
RA   Gugneja S., Virbasius J.V., Scarpulla R.C.;
RT   "Four structurally distinct, non-DNA-binding subunits of human nuclear
RT   respiratory factor 2 share a conserved transcriptional activation
RT   domain.";
RL   Mol. Cell. Biol. 15:102-111(1995).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA   Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P.,
RA   Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.;
RT   "Initial characterization of the human central proteome.";
RL   BMC Syst. Biol. 5:17-17(2011).
RN   [4]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-303, AND IDENTIFICATION
RP   BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Cervix carcinoma;
RX   PubMed=23186163; DOI=10.1021/pr300630k;
RA   Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA   Mohammed S.;
RT   "Toward a comprehensive characterization of a human cancer cell
RT   phosphoproteome.";
RL   J. Proteome Res. 12:260-271(2013).
CC   -!- FUNCTION: Transcription factor capable of interacting with purine
CC       rich repeats (GA repeats). Necessary for the expression of the
CC       Adenovirus E4 gene.
CC   -!- SUBUNIT: Heterotetramer of two alpha and two beta subunits.
CC   -!- INTERACTION:
CC       Q06547:GABPB1; NbExp=4; IntAct=EBI-638925, EBI-618165;
CC       Q8TAK5:GABPB2; NbExp=5; IntAct=EBI-638925, EBI-8468945;
CC       P51610:HCFC1; NbExp=2; IntAct=EBI-638925, EBI-396176;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SIMILARITY: Belongs to the ETS family. {ECO:0000305}.
DR   EMBL; D13318; BAA02575.1; -; mRNA.
DR   EMBL; U13044; AAA65706.1; -; mRNA.
DR   CCDS; CCDS13575.1; -.
DR   PIR; A48146; A48146.
DR   PIR; I38739; I38739.
DR   RefSeq; NP_001184226.1; NM_001197297.1.
DR   RefSeq; NP_002031.2; NM_002040.3.
DR   RefSeq; XP_005260995.1; XM_005260938.4.
DR   RefSeq; XP_011527822.1; XM_011529520.2.
DR   RefSeq; XP_011527823.1; XM_011529521.2.
DR   RefSeq; XP_016883802.1; XM_017028313.1.
DR   SMR; Q06546; -.
DR   BioGrid; 108826; 27.
DR   DIP; DIP-33980N; -.
DR   IntAct; Q06546; 12.
DR   MINT; Q06546; -.
DR   STRING; 9606.ENSP00000346886; -.
DR   iPTMnet; Q06546; -.
DR   PhosphoSitePlus; Q06546; -.
DR   BioMuta; GABPA; -.
DR   DMDM; 729553; -.
DR   EPD; Q06546; -.
DR   jPOST; Q06546; -.
DR   MassIVE; Q06546; -.
DR   MaxQB; Q06546; -.
DR   PaxDb; Q06546; -.
DR   PeptideAtlas; Q06546; -.
DR   PRIDE; Q06546; -.
DR   ProteomicsDB; 58457; -.
DR   DNASU; 2551; -.
DR   Ensembl; ENST00000354828; ENSP00000346886; ENSG00000154727.
DR   Ensembl; ENST00000400075; ENSP00000382948; ENSG00000154727.
DR   GeneID; 2551; -.
DR   KEGG; hsa:2551; -.
DR   UCSC; uc002ylx.5; human.
DR   CTD; 2551; -.
DR   DisGeNET; 2551; -.
DR   GeneCards; GABPA; -.
DR   HGNC; HGNC:4071; GABPA.
DR   HPA; CAB004411; -.
DR   HPA; HPA003258; -.
DR   HPA; HPA028791; -.
DR   MIM; 600609; gene.
DR   neXtProt; NX_Q06546; -.
DR   OpenTargets; ENSG00000154727; -.
DR   PharmGKB; PA28485; -.
DR   eggNOG; KOG3806; Eukaryota.
DR   eggNOG; ENOG410Z0ZF; LUCA.
DR   GeneTree; ENSGT00940000155799; -.
DR   HOGENOM; HOG000285952; -.
DR   InParanoid; Q06546; -.
DR   KO; K09441; -.
DR   OMA; DLFWTHL; -.
DR   OrthoDB; 526256at2759; -.
DR   PhylomeDB; Q06546; -.
DR   TreeFam; TF350537; -.
DR   Reactome; R-HSA-2151201; Transcriptional activation of mitochondrial biogenesis.
DR   ChiTaRS; GABPA; human.
DR   GeneWiki; GABPA; -.
DR   GenomeRNAi; 2551; -.
DR   Pharos; Q06546; -.
DR   PRO; PR:Q06546; -.
DR   Proteomes; UP000005640; Chromosome 21.
DR   Bgee; ENSG00000154727; Expressed in 202 organ(s), highest expression level in female gonad.
DR   ExpressionAtlas; Q06546; baseline and differential.
DR   Genevisible; Q06546; HS.
DR   GO; GO:0000790; C:nuclear chromatin; IDA:BHF-UCL.
DR   GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0033613; F:activating transcription factor binding; IPI:UniProtKB.
DR   GO; GO:0003682; F:chromatin binding; IEA:Ensembl.
DR   GO; GO:0003677; F:DNA binding; TAS:ProtInc.
DR   GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; TAS:ProtInc.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR   GO; GO:0046982; F:protein heterodimerization activity; IDA:MGI.
DR   GO; GO:0000978; F:RNA polymerase II proximal promoter sequence-specific DNA binding; IDA:NTNU_SB.
DR   GO; GO:0003713; F:transcription coactivator activity; TAS:ProtInc.
DR   GO; GO:0044212; F:transcription regulatory region DNA binding; IDA:MGI.
DR   GO; GO:0001825; P:blastocyst formation; IEA:Ensembl.
DR   GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR   GO; GO:1903351; P:cellular response to dopamine; IMP:CAFA.
DR   GO; GO:0007005; P:mitochondrion organization; TAS:Reactome.
DR   GO; GO:0045653; P:negative regulation of megakaryocyte differentiation; IEA:Ensembl.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:Ensembl.
DR   GO; GO:0010628; P:positive regulation of gene expression; IMP:CAFA.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; TAS:ProtInc.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 1.10.150.50; -; 1.
DR   InterPro; IPR000418; Ets_dom.
DR   InterPro; IPR024668; GABP_asu_N.
DR   InterPro; IPR003118; Pointed_dom.
DR   InterPro; IPR013761; SAM/pointed_sf.
DR   InterPro; IPR016312; TF_GA-bd_asu.
DR   InterPro; IPR029071; Ubiquitin-like_domsf.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00178; Ets; 1.
DR   Pfam; PF11620; GABP-alpha; 1.
DR   Pfam; PF02198; SAM_PNT; 1.
DR   PIRSF; PIRSF001703; GABP_alpha; 1.
DR   PRINTS; PR00454; ETSDOMAIN.
DR   SMART; SM00413; ETS; 1.
DR   SMART; SM00251; SAM_PNT; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF47769; SSF47769; 1.
DR   SUPFAM; SSF54236; SSF54236; 1.
DR   PROSITE; PS00345; ETS_DOMAIN_1; 1.
DR   PROSITE; PS00346; ETS_DOMAIN_2; 1.
DR   PROSITE; PS50061; ETS_DOMAIN_3; 1.
DR   PROSITE; PS51433; PNT; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; DNA-binding; Nucleus; Phosphoprotein; Polymorphism;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN         1    454       GA-binding protein alpha chain.
FT                                /FTId=PRO_0000204127.
FT   DOMAIN      168    251       PNT. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00762}.
FT   DNA_BIND    320    400       ETS. {ECO:0000255|PROSITE-
FT                                ProRule:PRU00237}.
FT   MOD_RES     303    303       Phosphoserine.
FT                                {ECO:0000244|PubMed:23186163}.
FT   VARIANT     291    291       A -> V (in dbSNP:rs2829897).
FT                                /FTId=VAR_020315.
FT   VARIANT     345    345       E -> K (in dbSNP:rs2829900).
FT                                /FTId=VAR_020316.
FT   CONFLICT    289    290       SS -> RC (in Ref. 2; AAA65706).
FT                                {ECO:0000305}.
FT   CONFLICT    440    440       A -> V (in Ref. 2; AAA65706).
FT                                {ECO:0000305}.
SQ   SEQUENCE   454 AA;  51295 MW;  1AF2ABBBC79191DD CRC64;
     MTKREAEELI EIEIDGTEKA ECTEESIVEQ TYAPAECVSQ AIDINEPIGN LKKLLEPRLQ
     CSLDAHEICL QDIQLDPERS LFDQGVKTDG TVQLSVQVIS YQGIEPKLNI LEIVKPADTV
     EVVIDPDAHH AESEAHLVEE AQVITLDGTK HITTISDETS EQVTRWAAAL EGYRKEQERL
     GIPYDPIQWS TDQVLHWVVW VMKEFSMTDI DLTTLNISGR ELCSLNQEDF FQRVPRGEIL
     WSHLELLRKY VLASQEQQMN EIVTIDQPVQ IIPASVQSAT PTTIKVINSS AKAAKVQRAP
     RISGEDRSSP GNRTGNNGQI QLWQFLLELL TDKDARDCIS WVGDEGEFKL NQPELVAQKW
     GQRKNKPTMN YEKLSRALRY YYDGDMICKV QGKRFVYKFV CDLKTLIGYS AAELNRLVTE
     CEQKKLAKMQ LHGIAQPVTA VALATASLQT EKDN
//
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