ID GDL75_ARATH Reviewed; 385 AA.
AC Q9FNP2;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 106.
DE RecName: Full=GDSL esterase/lipase At5g08460;
DE EC=3.1.1.-;
DE AltName: Full=Extracellular lipase At5g08460;
DE Flags: Precursor;
GN OrderedLocusNames=At5g08460; ORFNames=F8L15.13, MAH20.2;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP REVIEW.
RX PubMed=15522763; DOI=10.1016/j.plipres.2004.09.002;
RA Akoh C.C., Lee G.-C., Liaw Y.-C., Huang T.-H., Shaw J.-F.;
RT "GDSL family of serine esterases/lipases.";
RL Prog. Lipid Res. 43:534-552(2004).
RN [4]
RP GENE FAMILY.
RX PubMed=18819574; DOI=10.3923/pjbs.2008.763.767;
RA Ling H.;
RT "Sequence analysis of GDSL lipase gene family in Arabidopsis thaliana.";
RL Pak. J. Biol. Sci. 11:763-767(2008).
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC {ECO:0000305}.
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DR EMBL; AB006697; BAB09995.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91306.1; -; Genomic_DNA.
DR RefSeq; NP_196463.1; NM_120931.2.
DR AlphaFoldDB; Q9FNP2; -.
DR SMR; Q9FNP2; -.
DR STRING; 3702.Q9FNP2; -.
DR iPTMnet; Q9FNP2; -.
DR PaxDb; 3702-AT5G08460-1; -.
DR ProteomicsDB; 247107; -.
DR EnsemblPlants; AT5G08460.1; AT5G08460.1; AT5G08460.
DR GeneID; 830745; -.
DR Gramene; AT5G08460.1; AT5G08460.1; AT5G08460.
DR KEGG; ath:AT5G08460; -.
DR Araport; AT5G08460; -.
DR TAIR; AT5G08460; -.
DR eggNOG; ENOG502QSW9; Eukaryota.
DR HOGENOM; CLU_015101_0_0_1; -.
DR InParanoid; Q9FNP2; -.
DR OMA; STDRDIM; -.
DR OrthoDB; 1012174at2759; -.
DR PhylomeDB; Q9FNP2; -.
DR BioCyc; ARA:AT5G08460-MONOMER; -.
DR PRO; PR:Q9FNP2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FNP2; baseline and differential.
DR Genevisible; Q9FNP2; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0016788; F:hydrolase activity, acting on ester bonds; IEA:InterPro.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR CDD; cd01837; SGNH_plant_lipase_like; 1.
DR Gene3D; 3.40.50.1110; SGNH hydrolase; 1.
DR InterPro; IPR001087; GDSL.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR InterPro; IPR035669; SGNH_plant_lipase-like.
DR PANTHER; PTHR45650; GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED; 1.
DR PANTHER; PTHR45650:SF32; GDSL-LIKE LIPASE_ACYLHYDROLASE; 1.
DR Pfam; PF00657; Lipase_GDSL; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein; Hydrolase; Lipid degradation; Lipid metabolism;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..35
FT /evidence="ECO:0000255"
FT CHAIN 36..385
FT /note="GDSL esterase/lipase At5g08460"
FT /id="PRO_0000367415"
FT ACT_SITE 56
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 350
FT /evidence="ECO:0000250"
FT ACT_SITE 353
FT /evidence="ECO:0000250"
FT CARBOHYD 218
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 285
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 368
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 378
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 385 AA; 42417 MW; 75873A7DACC830AC CRC64;
MHDSEIFKFK DMMMMSCTVQ TLVLVPWFLV VFVLAGGEDS SETTAMFPAM FVFGDSLVDN
GNNNHLNSLA RSNYLPYGID FAGNQPTGRF SNGKTIVDFI GELLGLPEIP AFMDTVDGGV
DILHGVNYAS AAGGILEETG RHLGERFSMG RQVENFEKTL MEISRSMRKE SVKEYMAKSL
VVVSLGNNDY INNYLKPRLF LSSSIYDPTS FADLLLSNFT THLLELYGKG FRKFVIAGVG
PLGCIPDQLA AQAALPGECV EAVNEMAELF NNRLVSLVDR LNSDNKTASE AIFVYGNTYG
AAVDILTNPF NYGFEVTDRG CCGVGRNRGE ITCLPLAVPC AFRDRHVFWD AFHPTQAFNL
IIALRAFNGS KSDCYPINLS QLSRL
//
