ID GEFR_DICDI Reviewed; 1721 AA.
AC Q8SSQ0; Q555R4;
DT 22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 24-JAN-2024, entry version 119.
DE RecName: Full=Ras guanine nucleotide exchange factor R;
DE AltName: Full=RasGEF domain-containing protein R;
GN Name=gefR; Synonyms=rasGEFR; ORFNames=DDB_G0274605;
OS Dictyostelium discoideum (Social amoeba).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=AX4;
RX PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT roles.";
RL Genome Biol. 6:R68.1-R68.12(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [4]
RP NOMENCLATURE.
RC STRAIN=AX3;
RX PubMed=8793298; DOI=10.1016/s0960-9822(09)00453-9;
RA Insall R.H., Borleis J., Devreotes P.N.;
RT "The aimless RasGEF is required for processing of chemotactic signals
RT through G-protein-coupled receptors in Dictyostelium.";
RL Curr. Biol. 6:719-729(1996).
RN [5]
RP PHOSPHORYLATION, AND DISRUPTION PHENOTYPE.
RX PubMed=15352238; DOI=10.1002/pmic.200300788;
RA Secko D.M., Insall R.H., Spiegelman G.B., Weeks G.;
RT "The identification of Dictyostelium phosphoproteins altered in response to
RT the activation of RasG.";
RL Proteomics 4:2629-2639(2004).
RN [6]
RP FUNCTION.
RX PubMed=17380187; DOI=10.1038/sj.embor.7400936;
RA Kae H., Kortholt A., Rehmann H., Insall R.H., Van Haastert P.J.,
RA Spiegelman G.B., Weeks G.;
RT "Cyclic AMP signalling in Dictyostelium: G-proteins activate separate Ras
RT pathways using specific RasGEFs.";
RL EMBO Rep. 8:477-482(2007).
CC -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP. May also play
CC a role in the activation of rasG. {ECO:0000269|PubMed:17380187}.
CC -!- DEVELOPMENTAL STAGE: Expressed during development; especially between
CC 8-18 hours of development. Expressed (at protein level).
CC {ECO:0000269|PubMed:16086850}.
CC -!- PTM: Phosphorylated on threonine residues.
CC {ECO:0000269|PubMed:15352238}.
CC -!- DISRUPTION PHENOTYPE: Absence of the phosphoprotein gefR.
CC {ECO:0000269|PubMed:15352238}.
CC -!- CAUTION: Was originally (PubMed:8793298) named RasGEFA, as it was the
CC second RasGEF, after aimless, to be identified there. When aimless,
CC aleA, was renamed gefA to make the nomenclature consistent and
CC systematic, RasGEFA was renamed gefR. {ECO:0000305|PubMed:8793298}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY160106; AAN46886.1; -; Genomic_DNA.
DR EMBL; AAFI02000012; EAL70195.1; -; Genomic_DNA.
DR RefSeq; XP_643999.1; XM_638907.1.
DR AlphaFoldDB; Q8SSQ0; -.
DR SMR; Q8SSQ0; -.
DR STRING; 44689.Q8SSQ0; -.
DR PaxDb; 44689-DDB0185198; -.
DR EnsemblProtists; EAL70195; EAL70195; DDB_G0274605.
DR GeneID; 8619425; -.
DR KEGG; ddi:DDB_G0274605; -.
DR dictyBase; DDB_G0274605; gefR.
DR eggNOG; KOG3417; Eukaryota.
DR HOGENOM; CLU_240268_0_0_1; -.
DR InParanoid; Q8SSQ0; -.
DR OMA; QKRELIC; -.
DR PRO; PR:Q8SSQ0; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:dictyBase.
DR GO; GO:0007265; P:Ras protein signal transduction; IDA:dictyBase.
DR GO; GO:1903013; P:response to differentiation-inducing factor 1; HDA:dictyBase.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR Gene3D; 1.20.870.10; Son of sevenless (SoS) protein Chain: S domain 1; 1.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; GUANINE NUCLEOTIDE EXCHANGE FACTOR; 1.
DR PANTHER; PTHR23113:SF162; RAS GUANINE NUCLEOTIDE EXCHANGE FACTOR R; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Guanine-nucleotide releasing factor; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..1721
FT /note="Ras guanine nucleotide exchange factor R"
FT /id="PRO_0000384476"
FT DOMAIN 1291..1411
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT DOMAIN 1447..1676
FT /note="Ras-GEF"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT REGION 213..232
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 445..515
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 551..701
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 716..766
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 797..837
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 929..981
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 148..279
FT /evidence="ECO:0000255"
FT COILED 802..831
FT /evidence="ECO:0000255"
FT COMPBIAS 215..232
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 451..515
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 551..582
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 583..599
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 604..691
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 720..766
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 929..980
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1721 AA; 193133 MW; 68EA5A422CAFC8EC CRC64;
MKEEEIANLG PQEYFNYLEG INKKIIIELK TVANIDTAKD TDNLQNILIE SSLALEHNIR
NLLYFVDKCP ISYDQIVHYH SHFKEVVKIF FQVCQDLIKA AQALILNSCD YLTTSNFAKC
RGEAIKTIKN TMATCSNFEK LYYNPDLQLE DEVDLVHQQQ QKEQEQQQKE QEQYEQEQLI
LKQLKEQELK KQQEQQQQQQ QQQQQQQQQQ QQQQQKHQEE KEKNDQKEKE EKLKKQLLAE
QQKQKQEELE RQQQQLQQQQ QLLQQQQQLL QQQQQQQRSD PIIIPPITLK TSQSDLLSGG
LSPKVNSPGS PNLGKFTESA ERVANFDRGT ANIILNMNKI KEISLSGDSE QAPEIVTAAR
IISENIAIIS KELRYKTLGT NLSEHFVSVV QVARLAIKNK SDQFYQDQLE LAIEKFNDTM
RKIIYSVKSI SKSSMNLRQF GVDESSLGRS PVVSPEKSIS PSFTSSTSER ILSHEIKNHN
NNNNNYNNSS TNNLQTSFST PSLSSNHSQQ PNQQPLQSPL LINQLQSTSS SSSSSSSNLS
NSLNSIQLPQ ATTTTTTATS PSTSTSTSTS TSPNSSSLSI SDQDKQLKRK EKQHQIVIPK
DVKDSVANQQ QQQQQQQNGT TSPRNNESSV TAATTTTTST TASITTNVNT IPNFPPNKQL
PATPTTGTPS TSTPTPQTPT STSQNDKQNE NNNKENFVDK QKTLGKLFSK FVHKKRTPLP
GFDSSNSSSP SNNSNTTNSS SHSSTNSSPM ETSPGVESPK ITKSPSQNNI LVDSLDIGSD
NQQQQQDKLT TTTSTTTITI NNNNNNNNNN NNNNNNNNNN IQQQQQQQQQ IPTTPNKLSN
SIAMNSSNRL MTPKKERSFT IGLVSGKHSC VVVETPKSKK LFQHESAKQI LSIISMNFPS
FEKEFQMQNS EVISNIIEQI GNVIKNYHDE VSESSSSSSS STTSPNNINT PSDCSPILNS
ENSKDSHNLS SINNSSYDPV SPALRKMYEN GSYDSKEHSR SSIQSKITRK LGTIRKKGPS
PFNMLLNSST GSLSSLYLDQ LNGGNGDSSS SSSSSYNLVG GSGISGYDNQ NEIDASEHLV
STSSLFTEEA IELVSKCFEA SIIVNHSGGT SSEIKALFSS TLDHLESMLQ SSVNFGKIDP
SSFCMTFLKN IRQHIPTMKS KGGKCDPTLV TAATKLFRHT ILSNLDKSIH SLCHSVRVLA
IQMTIIVISI SAKPWDISSQ LQLFASAKSF IDSLVSLLDA VENKIYISTN TNIQDDVEVP
TIDDTEDTNI WEEADSPLTF GWISDEGSKT GRYVPKAGTL NKLISALTQD NKHDISRYTK
TFLLTYQSFT NPWKLMEKLI QRYNVPLDEK PDVRATTQLR VVSFMQTWIE RNFNDFDDQL
IGQLKEFRTR LLMDNNNDLA VILGGLIKKK EAERSLSKER STNHLTFPEL MIPDGQKSPT
ALFLLLNESE IARQLTLIDF NIFSKIQPTE LLDQSWNKDS LKFKSPNVIE MINRANKFSF
WVSSQILWQE DIEERVKVFE KFILISKYLR EMNNFNTLLA IFTGLNTAPI LRLKKTFALL
SPNSLSIYNS LEKLMNSSGS YKNYRSVSKN PPLLPYLPVI LSDLTFMEDG NPDKINNLIN
FQKRELICRV ISEVQQCQQQ TKYEFPVVEP IHTLLTELPS STPSELYQLS LIREPRETNQ
SNANSSISSG SNFLSNSSNH NIGNNNGFDT LKKYYKSSNN N
//
