ID GERAB_BACSU Reviewed; 365 AA.
AC P07869;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 27-MAR-2024, entry version 140.
DE RecName: Full=Spore germination protein A2;
GN Name=gerAB; Synonyms=gerA2; OrderedLocusNames=BSU33060;
OS Bacillus subtilis (strain 168).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=3110007; DOI=10.1016/0378-1119(87)90468-9;
RA Zuberi A.R., Moir A., Feavers I.M.;
RT "The nucleotide sequence and gene organization of the gerA spore
RT germination operon of Bacillus subtilis 168.";
RL Gene 51:1-11(1987).
RN [2]
RP SEQUENCE REVISION.
RA Moir A.;
RL Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9639930; DOI=10.1099/00221287-144-6-1593;
RA Wipat A., Brignell C.S., Guy J.B., Rose M., Emmerson P.T., Harwood C.R.;
RT "The yvsA-yvqA (293 degrees - 289 degrees) region of the Bacillus subtilis
RT chromosome containing genes involved in metal ion uptake and a putative
RT sigma factor.";
RL Microbiology 144:1593-1600(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [5]
RP SIMILARITY TO OTHER MEMBER OF THE FAMILY.
RX PubMed=8382989; DOI=10.1002/pro.5560020103;
RA Reizer J., Finley K., Kakuda D., McLeod C.L., Reizer A., Saier M.H. Jr.;
RT "Mammalian integral membrane receptors are homologous to facilitators and
RT antiporters of yeast, fungi, and eubacteria.";
RL Protein Sci. 2:20-30(1993).
CC -!- FUNCTION: Involved in the germinative response to L-alanine. Could be
CC an amino acid transporter. Forms a complex at the inner spore membrane
CC which acts as a receptor for L-alanine, thus is involved in the
CC stimulation of germination in response to alanine. Can stimulate
CC germination in the absence of gerD and gerK gene products (fructose and
CC glucose receptors, respectively), but the response is improved in their
CC presence.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- DEVELOPMENTAL STAGE: Expressed in the forespore compartment of the
CC developing sporangium.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. Spore germination protein (SGP) (TC 2.A.3.9) family.
CC {ECO:0000305}.
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DR EMBL; M16189; AAA92781.1; -; Genomic_DNA.
DR EMBL; AJ223978; CAA11747.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15296.1; -; Genomic_DNA.
DR PIR; F69629; F69629.
DR RefSeq; NP_391186.1; NC_000964.3.
DR RefSeq; WP_009968145.1; NZ_JNCM01000033.1.
DR AlphaFoldDB; P07869; -.
DR SMR; P07869; -.
DR STRING; 224308.BSU33060; -.
DR TCDB; 2.A.3.9.1; the amino acid-polyamine-organocation (apc) family.
DR PaxDb; 224308-BSU33060; -.
DR EnsemblBacteria; CAB15296; CAB15296; BSU_33060.
DR GeneID; 935948; -.
DR KEGG; bsu:BSU33060; -.
DR PATRIC; fig|224308.179.peg.3584; -.
DR eggNOG; COG0814; Bacteria.
DR InParanoid; P07869; -.
DR OrthoDB; 2716906at2; -.
DR PhylomeDB; P07869; -.
DR BioCyc; BSUB:BSU33060-MONOMER; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR GO; GO:0009847; P:spore germination; IEA:InterPro.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004761; Spore_GerAB.
DR NCBIfam; TIGR00912; 2A0309; 1.
DR PANTHER; PTHR34975; SPORE GERMINATION PROTEIN A2; 1.
DR PANTHER; PTHR34975:SF2; SPORE GERMINATION PROTEIN A2; 1.
DR Pfam; PF03845; Spore_permease; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Cell membrane; Germination; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..365
FT /note="Spore germination protein A2"
FT /id="PRO_0000054255"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 250..270
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 275..295
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 365 AA; 41459 MW; EB4901978C7CC1D6 CRC64;
MSQKQTPLKL NTFQGISIVA NTMLGAGLLT LPRALTTKAN TPDGWITLIL EGFIFIFFIY
LNTLIQKKHQ YPSLFEYLKE GLGKWIGSII GLLICGYFLG VASFETRAMA EMVKFFLLER
TPIQVIILTF ICCGIYLMVG GLSDVSRLFP FYLTVTIIIL LIVFGISFKI FDINNLRPVL
GEGLGPIANS LTVVSISFLG MEVMLFLPEH MKKKKYTFRY ASLGFLIPII LYILTYIIVV
GALTAPEVKT LIWPTISLFQ SFELKGIFIE RFESFLLVVW IIQFFTTFVI YGYFAANGLK
KTFGLSTKTS MVIIGITVFY FSLWPDDANQ VMMYSDYLGY IFVSLFLLPF ILFFIVALKR
RITTK
//
