ID GERBB_BACSU Reviewed; 368 AA.
AC P39570;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 2.
DT 27-MAR-2024, entry version 133.
DE RecName: Full=Spore germination protein B2;
GN Name=gerBB; OrderedLocusNames=BSU35810;
OS Bacillus subtilis (strain 168).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8012571; DOI=10.1099/00221287-140-3-471;
RA Corfe B.M., Sammons R.L., Smith D.A., Maueel C.;
RT "The gerB region of the Bacillus subtilis 168 chromosome encodes a
RT homologue of the gerA spore germination operon.";
RL Microbiology 140:471-478(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 119.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
CC -!- FUNCTION: Involved in the response to the germinative mixture of L-
CC asparagine, glucose, fructose and potassium ions (AGFK). Could be an
CC amino acid transporter. Cannot stimulate germination in the absence of
CC gerD and gerK gene products (fructose and glucose receptors,
CC respectively).
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- DEVELOPMENTAL STAGE: Expressed in the forespore compartment of the
CC developing sporangium.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. Spore germination protein (SGP) (TC 2.A.3.9) family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L16960; AAA22467.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15598.2; -; Genomic_DNA.
DR PIR; I39856; I39856.
DR RefSeq; NP_391462.2; NC_000964.3.
DR RefSeq; WP_003244581.1; NZ_JNCM01000034.1.
DR AlphaFoldDB; P39570; -.
DR SMR; P39570; -.
DR STRING; 224308.BSU35810; -.
DR TCDB; 2.A.3.9.2; the amino acid-polyamine-organocation (apc) family.
DR PaxDb; 224308-BSU35810; -.
DR EnsemblBacteria; CAB15598; CAB15598; BSU_35810.
DR GeneID; 936827; -.
DR KEGG; bsu:BSU35810; -.
DR PATRIC; fig|224308.179.peg.3877; -.
DR eggNOG; COG0814; Bacteria.
DR InParanoid; P39570; -.
DR OrthoDB; 2661055at2; -.
DR PhylomeDB; P39570; -.
DR BioCyc; BSUB:BSU35810-MONOMER; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR GO; GO:0009847; P:spore germination; IEA:InterPro.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004761; Spore_GerAB.
DR NCBIfam; TIGR00912; 2A0309; 1.
DR PANTHER; PTHR34975; SPORE GERMINATION PROTEIN A2; 1.
DR PANTHER; PTHR34975:SF2; SPORE GERMINATION PROTEIN A2; 1.
DR Pfam; PF03845; Spore_permease; 1.
DR PIRSF; PIRSF006060; AA_transporter; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Cell membrane; Germination; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..368
FT /note="Spore germination protein B2"
FT /id="PRO_0000054256"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..165
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 187..207
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 217..237
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 119
FT /note="P -> R (in Ref. 1; AAA22467)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 368 AA; 41650 MW; AB6BFC9512B00806 CRC64;
MRKSEHKLTF MQTLIMISST LIGAGVLTLP RSAAETGSPS GWLMILLQGV IFIIIVLLFL
PFLQKNSGKT LFKLNSIVAG KFIGFLLNLY ICLYFIGIVC FQARILGEVV GFFLLKNTPM
AVVVFIFLAV AIYHVGGGVY SIAKVYAYIF PITLIIFMML LMFSFRLFQL DFIRPVFEGG
YQSFFSLFPK TLLYFSGFEI IFYLVPFMRD PKQVKKAVAL GIATSTLFYS ITLLIVIGCM
TVAEAKTVTW PTISLIHALE VPGIFIERFD LFLQLTWTAQ QFACMLGSFK GAHIGLTEIF
HLKNKNNAWL LTAMLAATFF ITMYPKDLND VFYYGTLLGY AFLIVITIPF FVWFLSWIQK
KIGRGQLQ
//
