ID GHRHR_MOUSE Reviewed; 423 AA.
AC P32082; Q0VB62;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 24-JAN-2024, entry version 174.
DE RecName: Full=Growth hormone-releasing hormone receptor;
DE Short=GHRH receptor;
DE AltName: Full=Growth hormone-releasing factor receptor;
DE Short=GRF receptor;
DE Short=GRFR;
DE Flags: Precursor;
GN Name=Ghrhr; Synonyms=Grfr;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1334535; DOI=10.1038/360765a0;
RA Lin C.R., Lin S.-C., Chang C.P., Rosenfeld M.G.;
RT "Pit-1-dependent expression of the receptor for growth hormone releasing
RT factor mediates pituitary cell growth.";
RL Nature 360:765-768(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP VARIANT LIT GLY-60.
RX PubMed=8391647; DOI=10.1038/364208a0;
RA Lin S.-C., Lin C.R., Gukovsky I., Lusis A.J., Sawchenko P.E.,
RA Rosenfeld M.G.;
RT "Molecular basis of the little mouse phenotype and implications for cell
RT type-specific growth.";
RL Nature 364:208-213(1993).
CC -!- FUNCTION: Receptor for GRF, coupled to G proteins which activate
CC adenylyl cyclase. Stimulates somatotroph cell growth, growth hormone
CC gene transcription and growth hormone secretion.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Pituitary gland.
CC -!- DISEASE: Note=Little (lit) mice exhibits anterior pituitary hypoplasia.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 2 family.
CC {ECO:0000305}.
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DR EMBL; L07379; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; BC120748; AAI20749.1; -; mRNA.
DR EMBL; BC120774; AAI20775.1; -; mRNA.
DR CCDS; CCDS20165.1; -.
DR PIR; S29753; S29753.
DR RefSeq; NP_001003685.2; NM_001003685.3.
DR AlphaFoldDB; P32082; -.
DR SMR; P32082; -.
DR BioGRID; 199917; 1.
DR STRING; 10090.ENSMUSP00000068120; -.
DR GlyCosmos; P32082; 2 sites, No reported glycans.
DR GlyGen; P32082; 2 sites.
DR iPTMnet; P32082; -.
DR PhosphoSitePlus; P32082; -.
DR PaxDb; 10090-ENSMUSP00000068120; -.
DR Antibodypedia; 3360; 348 antibodies from 31 providers.
DR DNASU; 14602; -.
DR Ensembl; ENSMUST00000063578.6; ENSMUSP00000068120.4; ENSMUSG00000004654.7.
DR GeneID; 14602; -.
DR KEGG; mmu:14602; -.
DR UCSC; uc009cas.2; mouse.
DR AGR; MGI:95710; -.
DR CTD; 2692; -.
DR MGI; MGI:95710; Ghrhr.
DR VEuPathDB; HostDB:ENSMUSG00000004654; -.
DR eggNOG; KOG4564; Eukaryota.
DR GeneTree; ENSGT00940000159858; -.
DR HOGENOM; CLU_002753_4_4_1; -.
DR InParanoid; P32082; -.
DR OMA; RAWGACI; -.
DR OrthoDB; 5252868at2759; -.
DR PhylomeDB; P32082; -.
DR TreeFam; TF315710; -.
DR Reactome; R-MMU-418555; G alpha (s) signalling events.
DR Reactome; R-MMU-420092; Glucagon-type ligand receptors.
DR BioGRID-ORCS; 14602; 2 hits in 79 CRISPR screens.
DR PRO; PR:P32082; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; P32082; Protein.
DR Bgee; ENSMUSG00000004654; Expressed in pituitary gland and 19 other cell types or tissues.
DR ExpressionAtlas; P32082; baseline and differential.
DR Genevisible; P32082; MM.
DR GO; GO:0009986; C:cell surface; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0016020; C:membrane; IDA:MGI.
DR GO; GO:0005637; C:nuclear inner membrane; ISO:MGI.
DR GO; GO:0016363; C:nuclear matrix; ISO:MGI.
DR GO; GO:0005640; C:nuclear outer membrane; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0042383; C:sarcolemma; ISO:MGI.
DR GO; GO:0030141; C:secretory granule; ISO:MGI.
DR GO; GO:0008528; F:G protein-coupled peptide receptor activity; IBA:GO_Central.
DR GO; GO:0019838; F:growth factor binding; ISO:MGI.
DR GO; GO:0016520; F:growth hormone-releasing hormone receptor activity; IMP:MGI.
DR GO; GO:0017046; F:peptide hormone binding; IPI:BHF-UCL.
DR GO; GO:0021984; P:adenohypophysis development; IMP:MGI.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IMP:MGI.
DR GO; GO:0019933; P:cAMP-mediated signaling; ISO:MGI.
DR GO; GO:0048469; P:cell maturation; IMP:MGI.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR GO; GO:0032869; P:cellular response to insulin stimulus; IDA:MGI.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:MGI.
DR GO; GO:0051649; P:establishment of localization in cell; IMP:MGI.
DR GO; GO:0030252; P:growth hormone secretion; IMP:MGI.
DR GO; GO:0042445; P:hormone metabolic process; IMP:MGI.
DR GO; GO:0046879; P:hormone secretion; IMP:MGI.
DR GO; GO:0007595; P:lactation; IMP:MGI.
DR GO; GO:0030879; P:mammary gland development; IMP:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:BHF-UCL.
DR GO; GO:0046010; P:positive regulation of circadian sleep/wake cycle, non-REM sleep; ISO:MGI.
DR GO; GO:0060124; P:positive regulation of growth hormone secretion; IMP:MGI.
DR GO; GO:0046887; P:positive regulation of hormone secretion; IMP:MGI.
DR GO; GO:0040018; P:positive regulation of multicellular organism growth; IMP:MGI.
DR GO; GO:0043567; P:regulation of insulin-like growth factor receptor signaling pathway; IMP:MGI.
DR GO; GO:0033143; P:regulation of intracellular steroid hormone receptor signaling pathway; IMP:MGI.
DR GO; GO:0051246; P:regulation of protein metabolic process; IGI:MGI.
DR GO; GO:0043627; P:response to estrogen; ISO:MGI.
DR GO; GO:0051384; P:response to glucocorticoid; ISO:MGI.
DR GO; GO:0032868; P:response to insulin; ISO:MGI.
DR GO; GO:0060133; P:somatotropin secreting cell development; IMP:MGI.
DR CDD; cd15270; 7tmB1_GHRHR; 1.
DR Gene3D; 4.10.1240.10; GPCR, family 2, extracellular hormone receptor domain; 1.
DR Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR InterPro; IPR017981; GPCR_2-like_7TM.
DR InterPro; IPR036445; GPCR_2_extracell_dom_sf.
DR InterPro; IPR001879; GPCR_2_extracellular_dom.
DR InterPro; IPR003288; GPCR_2_GHRH_rcpt.
DR InterPro; IPR000832; GPCR_2_secretin-like.
DR InterPro; IPR017983; GPCR_2_secretin-like_CS.
DR PANTHER; PTHR45620:SF14; GROWTH HORMONE-RELEASING HORMONE RECEPTOR; 1.
DR PANTHER; PTHR45620; PDF RECEPTOR-LIKE PROTEIN-RELATED; 1.
DR Pfam; PF00002; 7tm_2; 1.
DR Pfam; PF02793; HRM; 1.
DR PRINTS; PR01352; GHRHRECEPTOR.
DR PRINTS; PR00249; GPCRSECRETIN.
DR SMART; SM00008; HormR; 1.
DR SUPFAM; SSF81321; Family A G protein-coupled receptor-like; 1.
DR SUPFAM; SSF111418; Hormone receptor domain; 1.
DR PROSITE; PS00649; G_PROTEIN_RECEP_F2_1; 1.
DR PROSITE; PS00650; G_PROTEIN_RECEP_F2_2; 1.
DR PROSITE; PS50227; G_PROTEIN_RECEP_F2_3; 1.
DR PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disease variant; Disulfide bond; G-protein coupled receptor;
KW Glycoprotein; Membrane; Receptor; Reference proteome; Signal; Transducer;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..423
FT /note="Growth hormone-releasing hormone receptor"
FT /id="PRO_0000012829"
FT TOPO_DOM 23..130
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 152..167
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189..210
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 211..231
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 232..240
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..261
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 262..283
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..304
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 305..331
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 353..357
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 379..423
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 49
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 50
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 41..64
FT /evidence="ECO:0000250"
FT DISULFID 55..96
FT /evidence="ECO:0000250"
FT DISULFID 78..112
FT /evidence="ECO:0000250"
FT VARIANT 60
FT /note="D -> G (in lit)"
FT /evidence="ECO:0000269|PubMed:8391647"
FT CONFLICT 265..266
FT /note="LA -> HS (in Ref. 1; L07379)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 423 AA; 47004 MW; A2BC658E719A8033 CRC64;
MDGLMWATRI LCLLSLCGVT LGHLHLECDF ITQLRDDELA CLQAAEGTNN TSLGCPGTWD
GLLCWPPTGS GQWVSLPCPE FFSHFGSDTG FVKRDCTITG WSNPFPPYPV ACPVPLELLT
KEKSYFSTVK IIYTTGHSIS IVALCVAIAI LVALRRLHCP RNYIHTQLFA TFILKASAVF
LKDAAIFQGD STDHCSMSTV LCKVSVAISH LATMTNFSWL LAEAVYLSCL LASTSPRSKP
AFWWLVLAGW GLPVLCTGTW VGCKLAFEDT ECWDLDNSSP CWWIIKGPIV LSVGVNFGLF
LNIICILLRK LEPAQGGLHT RAQYWRLSKS TLLLIPLFGI HYIIFNFLPD SAGLDIRVPL
ELGLGSFQGF IVAVLYCFLN QEVRTEISRK WYGHDPELLP ARRTCTEWTT PPRSRLKVLT
SEC
//
