ID GL112_ARATH Reviewed; 223 AA.
AC Q9FMA6; F4KCZ5;
DT 29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 138.
DE RecName: Full=Putative germin-like protein subfamily 1 member 12;
DE Flags: Precursor;
GN OrderedLocusNames=At5g38960; ORFNames=K15E6.20, K15E6_140;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
CC -!- FUNCTION: May play a role in plant defense. Probably has no oxalate
CC oxidase activity even if the active site is conserved.
CC -!- SUBUNIT: Oligomer (believed to be a pentamer but probably hexamer).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the germin family. {ECO:0000305}.
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DR EMBL; AB009048; BAB08652.1; -; Genomic_DNA.
DR EMBL; CP002688; AED94379.2; -; Genomic_DNA.
DR RefSeq; NP_198712.2; NM_123258.2.
DR AlphaFoldDB; Q9FMA6; -.
DR SMR; Q9FMA6; -.
DR PaxDb; 3702-AT5G38960-1; -.
DR ProteomicsDB; 230454; -.
DR EnsemblPlants; AT5G38960.1; AT5G38960.1; AT5G38960.
DR GeneID; 833888; -.
DR Gramene; AT5G38960.1; AT5G38960.1; AT5G38960.
DR KEGG; ath:AT5G38960; -.
DR Araport; AT5G38960; -.
DR TAIR; AT5G38960; -.
DR eggNOG; ENOG502QQ4A; Eukaryota.
DR HOGENOM; CLU_015790_0_0_1; -.
DR InParanoid; Q9FMA6; -.
DR OMA; VRIDYGI; -.
DR OrthoDB; 1037850at2759; -.
DR PhylomeDB; Q9FMA6; -.
DR PRO; PR:Q9FMA6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FMA6; baseline and differential.
DR Genevisible; Q9FMA6; AT.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0030145; F:manganese ion binding; IEA:InterPro.
DR CDD; cd02241; cupin_OxOx; 1.
DR Gene3D; 2.60.120.10; Jelly Rolls; 1.
DR InterPro; IPR006045; Cupin_1.
DR InterPro; IPR001929; Germin.
DR InterPro; IPR019780; Germin_Mn-BS.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR PANTHER; PTHR31238:SF305; GERMIN-LIKE PROTEIN SUBFAMILY 1 MEMBER 12-RELATED; 1.
DR PANTHER; PTHR31238; GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3; 1.
DR Pfam; PF00190; Cupin_1; 1.
DR PRINTS; PR00325; GERMIN.
DR SMART; SM00835; Cupin_1; 1.
DR SUPFAM; SSF51182; RmlC-like cupins; 1.
DR PROSITE; PS00725; GERMIN; 1.
PE 3: Inferred from homology;
KW Apoplast; Disulfide bond; Glycoprotein; Manganese; Metal-binding;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..223
FT /note="Putative germin-like protein subfamily 1 member 12"
FT /id="PRO_0000010812"
FT DOMAIN 65..216
FT /note="Cupin type-1"
FT /evidence="ECO:0000255"
FT BINDING 114
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 116
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 121
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT BINDING 162
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000250"
FT CARBOHYD 81
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 145
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 34..51
FT /evidence="ECO:0000250"
SQ SEQUENCE 223 AA; 24152 MW; 966E3A93E02EA2D8 CRC64;
MNMKNLYLAI LYLLAASTLP FAIASDPSPL QDFCIGVNTP ANALFVNGKF CKDPKLVTAD
DFYFSGLDKA RTTESSPVGS NVTTVNVNQI PGLNTLGISL VRIDYGINGQ NPPHTHPRAT
EILLVQEGTL FVGFFSSFPE NRLFNKTLNK GDVFVFPEGL IHFQVNIGKQ PAVAFASLSS
QNPGVIIIGN TLFGSKPPID PNVLAKAFQL DPKVIIQLQK KFG
//
