ID GNAT3_BOVIN Reviewed; 354 AA.
AC P0C7Q4;
DT 01-JUL-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-2008, sequence version 1.
DT 24-JAN-2024, entry version 95.
DE RecName: Full=Guanine nucleotide-binding protein G(t) subunit alpha-3;
DE AltName: Full=Gustducin alpha-3 chain;
GN Name=GNAT3;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford;
RX PubMed=19390049; DOI=10.1126/science.1169588;
RG The bovine genome sequencing and analysis consortium;
RT "The genome sequence of taurine cattle: a window to ruminant biology and
RT evolution.";
RL Science 324:522-528(2009).
RN [2]
RP MUTAGENESIS OF GLY-352, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=9671782; DOI=10.1073/pnas.95.15.8933;
RA Ming D., Ruiz-Avila L., Margolskee R.F.;
RT "Characterization and solubilization of bitter-responsive receptors that
RT couple to gustducin.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:8933-8938(1998).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=16928202; DOI=10.1111/j.1469-7580.2006.00617.x;
RA Tizzano M., Merigo F., Sbarbati A.;
RT "Evidence of solitary chemosensory cells in a large mammal: the diffuse
RT chemosensory system in Bos taurus airways.";
RL J. Anat. 209:333-337(2006).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=17021831; DOI=10.1007/s00359-006-0168-8;
RA Fehr J., Meyer D., Widmayer P., Borth H.C., Ackermann F., Wilhelm B.,
RA Gudermann T., Boekhoff I.;
RT "Expression of the G-protein alpha-subunit gustducin in mammalian
RT spermatozoa.";
RL J. Comp. Physiol. A 193:21-34(2007).
CC -!- FUNCTION: Guanine nucleotide-binding protein (G protein) alpha subunit
CC playing a prominent role in bitter and sweet taste transduction as well
CC as in umami (monosodium glutamate, monopotassium glutamate, and inosine
CC monophosphate) taste transduction.
CC -!- SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma,
CC respectively GNAT3, GNB1 and GNG13 for Gustducin heterotrimer for
CC bitter taste transduction. The alpha chain contains the guanine
CC nucleotide binding site. Gustducin heterotrimer may also be composed of
CC GNAT3, GNB3 and GNG13.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in epithelial cells of taste buds of the
CC circumvallate, foliate and fungiform. Detected in various region of the
CC respiratory track. Expressed also in spermatozoa.
CC {ECO:0000269|PubMed:16928202, ECO:0000269|PubMed:17021831,
CC ECO:0000269|PubMed:9671782}.
CC -!- INDUCTION: By bitter compounds denatonium, quinine, strychnine,
CC nicotine, atropine, quinacrine and caffeic acid in presence of taste
CC membranes. {ECO:0000269|PubMed:9671782}.
CC -!- SIMILARITY: Belongs to the G-alpha family. G(i/o/t/z) subfamily.
CC {ECO:0000305}.
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DR EMBL; AAFC03016846; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAFC03016847; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001103452.1; NM_001109982.1.
DR PDB; 4A4M; X-ray; 3.30 A; B=344-354.
DR PDB; 5EN0; X-ray; 2.81 A; B=344-354.
DR PDBsum; 4A4M; -.
DR PDBsum; 5EN0; -.
DR AlphaFoldDB; P0C7Q4; -.
DR SMR; P0C7Q4; -.
DR STRING; 9913.ENSBTAP00000011395; -.
DR PaxDb; 9913-ENSBTAP00000011395; -.
DR Ensembl; ENSBTAT00000011395.6; ENSBTAP00000011395.6; ENSBTAG00000008641.6.
DR GeneID; 516262; -.
DR KEGG; bta:516262; -.
DR CTD; 346562; -.
DR VEuPathDB; HostDB:ENSBTAG00000008641; -.
DR VGNC; VGNC:29456; GNAT3.
DR eggNOG; KOG0082; Eukaryota.
DR GeneTree; ENSGT00940000161422; -.
DR InParanoid; P0C7Q4; -.
DR OMA; EDQRQLC; -.
DR OrthoDB; 2897309at2759; -.
DR Reactome; R-BTA-170670; Adenylate cyclase inhibitory pathway.
DR Reactome; R-BTA-381771; Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).
DR Reactome; R-BTA-392170; ADP signalling through P2Y purinoceptor 12.
DR Reactome; R-BTA-418594; G alpha (i) signalling events.
DR Reactome; R-BTA-9009391; Extra-nuclear estrogen signaling.
DR Reactome; R-BTA-9717207; Sensory perception of sweet, bitter, and umami (glutamate) taste.
DR Proteomes; UP000009136; Chromosome 4.
DR Bgee; ENSBTAG00000008641; Expressed in oocyte and 8 other cell types or tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005834; C:heterotrimeric G-protein complex; IBA:GO_Central.
DR GO; GO:0001917; C:photoreceptor inner segment; IBA:GO_Central.
DR GO; GO:0001750; C:photoreceptor outer segment; IBA:GO_Central.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IBA:GO_Central.
DR GO; GO:0031683; F:G-protein beta/gamma-subunit complex binding; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0001580; P:detection of chemical stimulus involved in sensory perception of bitter taste; IBA:GO_Central.
DR GO; GO:0050916; P:sensory perception of sweet taste; IBA:GO_Central.
DR GO; GO:0050917; P:sensory perception of umami taste; IEA:Ensembl.
DR CDD; cd00066; G-alpha; 1.
DR Gene3D; 1.10.400.10; GI Alpha 1, domain 2-like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR001408; Gprotein_alpha_I.
DR InterPro; IPR001019; Gprotein_alpha_su.
DR InterPro; IPR011025; GproteinA_insert.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR10218; GTP-BINDING PROTEIN ALPHA SUBUNIT; 1.
DR PANTHER; PTHR10218:SF66; GUANINE NUCLEOTIDE-BINDING PROTEIN G(T) SUBUNIT ALPHA-3; 1.
DR Pfam; PF00503; G-alpha; 1.
DR PRINTS; PR00318; GPROTEINA.
DR PRINTS; PR00441; GPROTEINAI.
DR SMART; SM00275; G_alpha; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF47895; Transducin (alpha subunit), insertion domain; 1.
DR PROSITE; PS51882; G_ALPHA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; GTP-binding; Lipoprotein; Magnesium;
KW Metal-binding; Myristate; Nucleotide-binding; Reference proteome;
KW Transducer.
FT INIT_MET 1
FT /note="Removed"
FT CHAIN 2..354
FT /note="Guanine nucleotide-binding protein G(t) subunit
FT alpha-3"
FT /id="PRO_0000342670"
FT DOMAIN 32..354
FT /note="G-alpha"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 35..48
FT /note="G1 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 173..181
FT /note="G2 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 196..205
FT /note="G3 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 265..272
FT /note="G4 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT REGION 324..329
FT /note="G5 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01230"
FT COMPBIAS 10..26
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 40..47
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 47
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 175..181
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 181
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 200..204
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 269..272
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT BINDING 326
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250"
FT MUTAGEN 352
FT /note="G->P: Loss of in vitro activation by denatonium due
FT to disruption of interaction with taste receptors."
FT /evidence="ECO:0000269|PubMed:9671782"
FT HELIX 345..350
FT /evidence="ECO:0007829|PDB:5EN0"
SQ SEQUENCE 354 AA; 40332 MW; E5A707336B6941A9 CRC64;
MGIGISSESK ESAKRSKELE KKLQEDAERD ARTVKLLLLG AGESGKSTIV KQMKIIHKNG
YSEQECMEFK AVIYSNTLQS ILAIVKAMAT LEIDYVNPRS AEDQQQLCAM ANTLEDGSMT
PELAEIIKRL WRDPGVQACF ERASEYQLND SAAYYLNDLD RIAAPGYVPN EQDVLHSRVK
TTGIIETQFS FKDLHFRMFD VGGQRSERKK WIHCFEGVTC IIFCAALSAY DMVLVEDEEV
NRMHESLHLF NSICNHKYFA TTSIVLFLNK KDLFQEKVTK VHLSICFPEY TGPNTFEDAG
NYIKNQFLDL NLKKEDKEIY SHMTCATDTQ NVKFVFDAVT DIIIKENLKD CGLF
//
