ID GRF5_ARATH Reviewed; 397 AA.
AC Q8L8A6; C0SVA9; Q9LVK4;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 27-MAR-2024, entry version 117.
DE RecName: Full=Growth-regulating factor 5;
DE Short=AtGRF5;
DE AltName: Full=Transcription activator GRF5;
GN Name=GRF5; OrderedLocusNames=At3g13960; ORFNames=MDC16.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), GENE FAMILY, NOMENCLATURE, AND
RP TISSUE SPECIFICITY.
RX PubMed=12974814; DOI=10.1046/j.1365-313x.2003.01862.x;
RA Kim J.H., Choi D., Kende H.;
RT "The AtGRF family of putative transcription factors is involved in leaf and
RT cotyledon growth in Arabidopsis.";
RL Plant J. 36:94-104(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-397 (ISOFORM 2).
RA Fujita M., Mizukado S., Seki M., Shinozaki K., Mitsuda N., Takiguchi Y.,
RA Takagi M.;
RT "ORF cloning and analysis of Arabidopsis transcription factor genes.";
RL Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION.
RX PubMed=15326298; DOI=10.1073/pnas.0405450101;
RA Kim J.H., Kende H.;
RT "A transcriptional coactivator, AtGIF1, is involved in regulating leaf
RT growth and morphology in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:13374-13379(2004).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, AND INTERACTION WITH
RP GIF1.
RX PubMed=15960617; DOI=10.1111/j.1365-313x.2005.02429.x;
RA Horiguchi G., Kim G.T., Tsukaya H.;
RT "The transcription factor AtGRF5 and the transcription coactivator AN3
RT regulate cell proliferation in leaf primordia of Arabidopsis thaliana.";
RL Plant J. 43:68-78(2005).
RN [7]
RP DEVELOPMENTAL STAGE.
RX PubMed=20023165; DOI=10.1242/dev.043067;
RA Rodriguez R.E., Mecchia M.A., Debernardi J.M., Schommer C., Weigel D.,
RA Palatnik J.F.;
RT "Control of cell proliferation in Arabidopsis thaliana by microRNA
RT miR396.";
RL Development 137:103-112(2010).
CC -!- FUNCTION: Transcription activator that plays a role in the regulation
CC of cell expansion in leaf and cotyledons tissues. Acts together with
CC GIF1 for the development of appropriate leaf size and shape through the
CC promotion and/or maintenance of cell proliferation activity in leaf
CC primordia. {ECO:0000269|PubMed:15326298, ECO:0000269|PubMed:15960617}.
CC -!- SUBUNIT: Interacts with GIF1. {ECO:0000269|PubMed:15960617}.
CC -!- INTERACTION:
CC Q8L8A6; Q39011: ASK7; NbExp=3; IntAct=EBI-1396652, EBI-1798250;
CC Q8L8A6; Q94ID6: ERF12; NbExp=3; IntAct=EBI-1396652, EBI-4446727;
CC Q8L8A6; Q8L8A5: GIF1; NbExp=4; IntAct=EBI-1396652, EBI-1396623;
CC Q8L8A6; Q9MAL9: GIF2; NbExp=3; IntAct=EBI-1396652, EBI-1396863;
CC Q8L8A6; Q93VH6: GIF3; NbExp=3; IntAct=EBI-1396652, EBI-15194507;
CC Q8L8A6; Q9C5X0: IAA34; NbExp=3; IntAct=EBI-1396652, EBI-3946459;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU01002}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8L8A6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8L8A6-2; Sequence=VSP_044146;
CC -!- TISSUE SPECIFICITY: Strongly expressed in actively growing and
CC developing tissues, such as roots, upper stems, and shoot tips
CC containing the shoot apical meristem (SAM) and flower buds. Also
CC expressed in mature flowers, but weakly expressed in mature stems and
CC leaves. {ECO:0000269|PubMed:12974814, ECO:0000269|PubMed:15960617}.
CC -!- DEVELOPMENTAL STAGE: Expressed during the early stages of leaf
CC development and expression decreases with the maturation of the leaf.
CC {ECO:0000269|PubMed:20023165}.
CC -!- DOMAIN: The QLQ domain and WRC domain may be involved in protein-
CC protein interaction and DNA-binding, respectively.
CC -!- DISRUPTION PHENOTYPE: Small and narrow leaves.
CC {ECO:0000269|PubMed:15960617}.
CC -!- MISCELLANEOUS: [Isoform 2]: May be due to intron retention.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the GRF family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AY102638; AAM52880.1; -; mRNA.
DR EMBL; AB019229; BAB02326.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75445.1; -; Genomic_DNA.
DR EMBL; AB493612; BAH30450.1; -; mRNA.
DR RefSeq; NP_188012.2; NM_112250.3. [Q8L8A6-1]
DR AlphaFoldDB; Q8L8A6; -.
DR BioGRID; 5943; 33.
DR IntAct; Q8L8A6; 33.
DR STRING; 3702.Q8L8A6; -.
DR PaxDb; 3702-AT3G13960-1; -.
DR ProteomicsDB; 247287; -. [Q8L8A6-1]
DR EnsemblPlants; AT3G13960.1; AT3G13960.1; AT3G13960. [Q8L8A6-1]
DR GeneID; 820609; -.
DR Gramene; AT3G13960.1; AT3G13960.1; AT3G13960. [Q8L8A6-1]
DR KEGG; ath:AT3G13960; -.
DR Araport; AT3G13960; -.
DR TAIR; AT3G13960; GRF5.
DR eggNOG; ENOG502RAHZ; Eukaryota.
DR HOGENOM; CLU_037908_1_0_1; -.
DR InParanoid; Q8L8A6; -.
DR OMA; FMDSSSY; -.
DR OrthoDB; 1209630at2759; -.
DR PhylomeDB; Q8L8A6; -.
DR PRO; PR:Q8L8A6; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8L8A6; baseline and differential.
DR Genevisible; Q8L8A6; AT.
DR GO; GO:0005634; C:nucleus; ISS:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR GO; GO:0009409; P:response to cold; IEP:TAIR.
DR GO; GO:0009739; P:response to gibberellin; IMP:TAIR.
DR InterPro; IPR014978; Gln-Leu-Gln_QLQ.
DR InterPro; IPR031137; GRF.
DR InterPro; IPR014977; WRC_dom.
DR PANTHER; PTHR31602; GROWTH-REGULATING FACTOR 5; 1.
DR PANTHER; PTHR31602:SF46; GROWTH-REGULATING FACTOR 5; 1.
DR Pfam; PF08880; QLQ; 1.
DR Pfam; PF08879; WRC; 1.
DR SMART; SM00951; QLQ; 1.
DR PROSITE; PS51666; QLQ; 1.
DR PROSITE; PS51667; WRC; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..397
FT /note="Growth-regulating factor 5"
FT /id="PRO_0000419296"
FT DOMAIN 16..51
FT /note="QLQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01001"
FT DOMAIN 81..125
FT /note="WRC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT REGION 108..172
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 197..217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 288..320
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 340..397
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 78..96
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT MOTIF 114..121
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01002"
FT COMPBIAS 122..172
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 348..371
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 374..397
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 397
FT /note="S -> N (in isoform 2)"
FT /evidence="ECO:0000303|Ref.4"
FT /id="VSP_044146"
SQ SEQUENCE 397 AA; 44696 MW; 706BE379D661DD43 CRC64;
MMSLSGSSGR TIGRPPFTPT QWEELEHQAL IYKYMVSGVP VPPELIFSIR RSLDTSLVSR
LLPHQSLGWG CYQMGFGRKP DPEPGRCRRT DGKKWRCSRE AYPDSKYCEK HMHRGRNRAR
KSLDQNQTTT TPLTSPSLSF TNNNNPSPTL SSSSSSNSSS TTYSASSSSM DAYSNSNRFG
LGGSSSNTRG YFNSHSLDYP YPSTSPKQQQ QTLHHASALS LHQNTNSTSQ FNVLASATDH
KDFRYFQGIG ERVGGVGERT FFPEASRSFQ DSPYHHHQQP LATVMNDPYH HCSTDHNKID
HHHTYSSSSS SQHLHHDHDH RQQQCFVLGA DMFNKPTRSV LANSSRQDQN QEEDEKDSSE
SSKKSLHHFF GEDWAQNKNS SDSWLDLSSH SRLDTGS
//
