ID H0EDJ6_GLAL7 Unreviewed; 774 AA.
AC H0EDJ6;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE SubName: Full=Putative Bifunctional P-450:NADPH-P450 reductase {ECO:0000313|EMBL:EHL03422.1};
GN ORFNames=M7I_0645 {ECO:0000313|EMBL:EHL03422.1};
OS Glarea lozoyensis (strain ATCC 74030 / MF5533).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Helotiales; Helotiaceae; Glarea.
OX NCBI_TaxID=1104152 {ECO:0000313|EMBL:EHL03422.1, ECO:0000313|Proteomes:UP000005446};
RN [1] {ECO:0000313|EMBL:EHL03422.1, ECO:0000313|Proteomes:UP000005446}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 74030 / MF5533 {ECO:0000313|Proteomes:UP000005446};
RX PubMed=22302591; DOI=10.1128/EC.05302-11;
RA Youssar L., Gruening B.A., Erxleben A., Guenther S., Huettel W.;
RT "Genome sequence of the fungus Glarea lozoyensis: the first genome sequence
RT of a species from the Helotiaceae family.";
RL Eukaryot. Cell 11:250-250(2012).
CC -!- COFACTOR:
CC Name=FAD; Xref=ChEBI:CHEBI:57692;
CC Evidence={ECO:0000256|ARBA:ARBA00001974};
CC -!- COFACTOR:
CC Name=FMN; Xref=ChEBI:CHEBI:58210;
CC Evidence={ECO:0000256|ARBA:ARBA00001917};
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413;
CC Evidence={ECO:0000256|ARBA:ARBA00001971};
CC -!- SIMILARITY: In the N-terminal section; belongs to the cytochrome P450
CC family. {ECO:0000256|ARBA:ARBA00010018}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EHL03422.1}.
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DR EMBL; AGUE01000010; EHL03422.1; -; Genomic_DNA.
DR AlphaFoldDB; H0EDJ6; -.
DR HOGENOM; CLU_001570_7_0_1; -.
DR InParanoid; H0EDJ6; -.
DR Proteomes; UP000005446; Unassembled WGS sequence.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:InterPro.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR CDD; cd06206; bifunctional_CYPOR; 1.
DR Gene3D; 3.40.50.360; -; 1.
DR Gene3D; 1.10.630.10; Cytochrome P450; 1.
DR Gene3D; 3.40.50.80; Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR InterPro; IPR003097; CysJ-like_FAD-binding.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR036396; Cyt_P450_sf.
DR InterPro; IPR017927; FAD-bd_FR_type.
DR InterPro; IPR008254; Flavodoxin/NO_synth.
DR InterPro; IPR001709; Flavoprot_Pyr_Nucl_cyt_Rdtase.
DR InterPro; IPR029039; Flavoprotein-like_sf.
DR InterPro; IPR039261; FNR_nucleotide-bd.
DR InterPro; IPR023173; NADPH_Cyt_P450_Rdtase_alpha.
DR InterPro; IPR001433; OxRdtase_FAD/NAD-bd.
DR InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR PANTHER; PTHR19384:SF127; BIFUNCTIONAL CYTOCHROME P450_NADPH--P450 REDUCTASE; 1.
DR PANTHER; PTHR19384; NITRIC OXIDE SYNTHASE-RELATED; 1.
DR Pfam; PF00667; FAD_binding_1; 1.
DR Pfam; PF00258; Flavodoxin_1; 1.
DR Pfam; PF00175; NAD_binding_1; 1.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00371; FPNCR.
DR SUPFAM; SSF48264; Cytochrome P450; 1.
DR SUPFAM; SSF52343; Ferredoxin reductase-like, C-terminal NADP-linked domain; 1.
DR SUPFAM; SSF52218; Flavoproteins; 1.
DR SUPFAM; SSF63380; Riboflavin synthase domain-like; 1.
DR PROSITE; PS51384; FAD_FR; 1.
DR PROSITE; PS50902; FLAVODOXIN_LIKE; 1.
PE 3: Inferred from homology;
KW Electron transport {ECO:0000256|ARBA:ARBA00022982};
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW FMN {ECO:0000256|ARBA:ARBA00022643}; NADP {ECO:0000256|ARBA:ARBA00022857};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000005446};
KW Transport {ECO:0000256|ARBA:ARBA00022982}.
FT DOMAIN 221..362
FT /note="Flavodoxin-like"
FT /evidence="ECO:0000259|PROSITE:PS50902"
FT DOMAIN 395..612
FT /note="FAD-binding FR-type"
FT /evidence="ECO:0000259|PROSITE:PS51384"
SQ SEQUENCE 774 AA; 84999 MW; E124DB0CD8101F61 CRC64;
MVSVLEESQT RGSLPTVLNM FRRSAQRKYD EEIAWMRGIC EEMIAHRRKN PLEKKDLLNA
MIHGKDPKTG CYMTDESIVD NILTFLVAGH ETTSGLLSFV FYLLIKNPAI MNLAREEVDK
VLGTGPITPQ HMNQFPYLEA ILKETLRLYP TAPAIAMAPV PETTEYPIFL AGGKYQLNKG
DNLLALLVKI QKDPLAFGDD AEEWKPERML EENFAKLPPN SWKPFGNGAR TCEALAQSLA
RVAGTRGFEV RVDPLDSATD DVPKSQPLII ICPSYEGQPP DNAAQFFGWL EKLKDGKSLQ
NVEFTVFGVG NRDWTSTFHK VPMNLDRLLA ANGGTRIAAL GLGDVAKGDI FNDFDYWQDN
VLWPAIQGSG SSTKNVVAEL DLEIDTSARS QTLRQDLQGA VVLSNKILTA LGVPEKRHIE
IQLPKGMNYT AGEYLAILPT NSQTNVRRVL KRFGLPADAM ITIRNVNAGS TLPATHPVSV
TDLLSSYVEL NQPATKRNIQ VLASLPTTPS TRTALLALTT NTTTKNTPPP SLLTLLETHP
TLPIPFQTYI SLLPPLRTRQ YSISSSPLAT PDVVSITFSI LPSGVASTYL ATLEPDDIMH
IAVKPTILFT LPEHMGKTPI IMIGAGAGIA PFLGFLQERA ALLNRNSELS LAPAWLFLGC
RAPEQDALFA DELRAYEDAG VVRVYYAYSQ EEGKSRGCRY VQDRVWAERG EIGGLIRGEG
KILVCGGQEL GEGVKGVVRR IYGEGRRGDG KADGIGEEDV ERWFDGLKGK GRGI
//
