ID H0EHX4_GLAL7 Unreviewed; 721 AA.
AC H0EHX4;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=Long-chain-alcohol oxidase {ECO:0000256|ARBA:ARBA00013125, ECO:0000256|PIRNR:PIRNR028937};
DE EC=1.1.3.20 {ECO:0000256|ARBA:ARBA00013125, ECO:0000256|PIRNR:PIRNR028937};
GN ORFNames=M7I_2117 {ECO:0000313|EMBL:EHL01767.1};
OS Glarea lozoyensis (strain ATCC 74030 / MF5533).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Leotiomycetes;
OC Helotiales; Helotiaceae; Glarea.
OX NCBI_TaxID=1104152 {ECO:0000313|EMBL:EHL01767.1, ECO:0000313|Proteomes:UP000005446};
RN [1] {ECO:0000313|EMBL:EHL01767.1, ECO:0000313|Proteomes:UP000005446}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 74030 / MF5533 {ECO:0000313|Proteomes:UP000005446};
RX PubMed=22302591; DOI=10.1128/EC.05302-11;
RA Youssar L., Gruening B.A., Erxleben A., Guenther S., Huettel W.;
RT "Genome sequence of the fungus Glarea lozoyensis: the first genome sequence
RT of a species from the Helotiaceae family.";
RL Eukaryot. Cell 11:250-250(2012).
CC -!- FUNCTION: Long-chain fatty alcohol oxidase involved in the omega-
CC oxidation pathway of lipid degradation.
CC {ECO:0000256|ARBA:ARBA00003842}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a long-chain primary fatty alcohol + O2 = a long-chain fatty
CC aldehyde + H2O2; Xref=Rhea:RHEA:22756, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:16240, ChEBI:CHEBI:17176, ChEBI:CHEBI:77396; EC=1.1.3.20;
CC Evidence={ECO:0000256|ARBA:ARBA00000920,
CC ECO:0000256|PIRNR:PIRNR028937};
CC -!- SIMILARITY: Belongs to the GMC oxidoreductase family.
CC {ECO:0000256|ARBA:ARBA00010790, ECO:0000256|PIRNR:PIRNR028937}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EHL01767.1}.
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DR EMBL; AGUE01000044; EHL01767.1; -; Genomic_DNA.
DR AlphaFoldDB; H0EHX4; -.
DR HOGENOM; CLU_008878_3_1_1; -.
DR InParanoid; H0EHX4; -.
DR Proteomes; UP000005446; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
DR GO; GO:0046577; F:long-chain-alcohol oxidase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 2.
DR InterPro; IPR003953; FAD-binding_2.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR000172; GMC_OxRdtase_N.
DR InterPro; IPR007867; GMC_OxRtase_C.
DR InterPro; IPR012400; Long_Oxdase.
DR PANTHER; PTHR46056; LONG-CHAIN-ALCOHOL OXIDASE; 1.
DR PANTHER; PTHR46056:SF4; LONG-CHAIN-ALCOHOL OXIDASE; 1.
DR Pfam; PF00890; FAD_binding_2; 1.
DR Pfam; PF05199; GMC_oxred_C; 1.
DR Pfam; PF00732; GMC_oxred_N; 1.
DR PIRSF; PIRSF028937; Lg_Ch_AO; 2.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
PE 3: Inferred from homology;
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002,
KW ECO:0000256|PIRNR:PIRNR028937};
KW Reference proteome {ECO:0000313|Proteomes:UP000005446};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
FT DOMAIN 220..251
FT /note="FAD-dependent oxidoreductase 2 FAD binding"
FT /evidence="ECO:0000259|Pfam:PF00890"
FT DOMAIN 268..476
FT /note="Glucose-methanol-choline oxidoreductase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00732"
FT DOMAIN 557..706
FT /note="Glucose-methanol-choline oxidoreductase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF05199"
FT ACT_SITE 653
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR028937-1"
SQ SEQUENCE 721 AA; 78787 MW; 0E55420755972431 CRC64;
MAAAEGPPLP IEVTDENVAN PFSDTQWTTL MSIMDTIIPS IQNDSSTDQN AHKSFSAEEF
NTAVNSLRDI LEKEHDIKIY EEYLNRKASD ESKFQNTLKH AFGKLLPESA RKGMAFILST
LDTRVGSMLL TGYTTSFHDQ PIHIREAILE SWRVSYLPTL NLVYKQMMVI AKNVWLNTSP
SFRELVGIPG GPDHVKLEKP FENDFLQFNS GTAPEIVETD VVIVGSGCGG GVCAKNIAEA
GHRVLVVDKA YHIPAAQLPL SEQNGLRKLF EGGAMLTSDD GSIAVAAGSA WGGGGTINWS
ASLQPQEFVR REWSQDKGLE VFESAEFQDC LDTVCKHMEG SRKLGFSAKA VPQNTGGKEH
YCGHCTLGCG SSEKQGPAVA WLPDAVRAGA QMVEGFRVDH VIFDEDDPTK ATGVKGLWTA
RDDTGEFDSE GRTTREVIVK AKKVIISSGT LWSPIILQNS GLRNYHIGRN LHLHPVNMLS
AIFPTSTRPW EGGILTSVCT TFENLDGLGH GAKLETTVML PSMFLVGFPW YSGLQFKRNA
LKFRNMNGFI SIARDRDTGR VYPDPTTGTP RVKYTPSVFD RKHILEGVIG LAKIAYIQGA
VEIHPFTSAG RPFIRDPNAN PGAGITDPHF QNWIKEFREG GNKPPATSFS SAHQMGTCRM
SRTEAEGVVD DKGRVWGCKG LYVADASVFP SASGVNPMVT NMAVAEWISR GVVGEMGKVE
V
//
