ID H0GPS1_SACCK Unreviewed; 567 AA.
AC H0GPS1;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=tRNA (uracil-O(2)-)-methyltransferase {ECO:0000256|ARBA:ARBA00017788, ECO:0000256|RuleBase:RU368004};
DE EC=2.1.1.211 {ECO:0000256|ARBA:ARBA00012795, ECO:0000256|RuleBase:RU368004};
GN ORFNames=VIN7_5000 {ECO:0000313|EMBL:EHN04390.1};
OS Saccharomyces cerevisiae x Saccharomyces kudriavzevii (strain VIN7)
OS (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=1095631 {ECO:0000313|EMBL:EHN04390.1, ECO:0000313|Proteomes:UP000009009};
RN [1] {ECO:0000313|EMBL:EHN04390.1, ECO:0000313|Proteomes:UP000009009}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VIN7 {ECO:0000313|EMBL:EHN04390.1,
RC ECO:0000313|Proteomes:UP000009009};
RX PubMed=22136070; DOI=10.1111/j.1567-1364.2011.00773.x;
RA Borneman A.R., Desany B.A., Riches D., Affourtit J.P., Forgan A.H.,
RA Pretorius I.S., Egholm M., Chambers P.J.;
RT "The genome sequence of the wine yeast VIN7 reveals an allotriploid hybrid
RT genome with Saccharomyces cerevisiae and Saccharomyces kudriavzevii
RT origins.";
RL FEMS Yeast Res. 12:88-96(2012).
CC -!- FUNCTION: Adenosyl-L-methionine (AdoMet)-dependent tRNA (uracil-O(2)-)-
CC methyltransferase. {ECO:0000256|RuleBase:RU368004}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-adenosyl-L-methionine + uridine(44) in tRNA(Ser) = 2'-O-
CC methyluridine(44) in tRNA(Ser) + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43100, Rhea:RHEA-COMP:10339, Rhea:RHEA-COMP:10340,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.211;
CC Evidence={ECO:0000256|ARBA:ARBA00000660,
CC ECO:0000256|RuleBase:RU368004};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|RuleBase:RU368004}.
CC -!- SIMILARITY: Belongs to the TRM44 family.
CC {ECO:0000256|ARBA:ARBA00009056, ECO:0000256|RuleBase:RU368004}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EHN04390.1}.
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DR EMBL; AGVY01000049; EHN04390.1; -; Genomic_DNA.
DR HOGENOM; CLU_018580_2_0_1; -.
DR PhylomeDB; H0GPS1; -.
DR Proteomes; UP000009009; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0141101; F:tRNA(Ser) (uridine(44)-2'-O-)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0030488; P:tRNA methylation; IEA:UniProtKB-UniRule.
DR InterPro; IPR011671; tRNA_uracil_MeTrfase.
DR PANTHER; PTHR21210:SF0; TRNA (URACIL-O(2)-)-METHYLTRANSFERASE-RELATED; 1.
DR PANTHER; PTHR21210; UNCHARACTERIZED; 1.
DR Pfam; PF07757; AdoMet_MTase; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|RuleBase:RU368004};
KW Methyltransferase {ECO:0000256|RuleBase:RU368004};
KW S-adenosyl-L-methionine {ECO:0000256|RuleBase:RU368004};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU368004};
KW tRNA processing {ECO:0000256|RuleBase:RU368004}.
SQ SEQUENCE 567 AA; 64861 MW; 7F355996E4FBFA3A CRC64;
MTGDGSAHIS KNNQNQHKDR FKFIVNDKSI LGPQWLSLYQ TDGKVTFAKS HFEQAMMNVI
REPNINSTVI LRADILKEIN HAAEAGSEPK FDESVLKKFE IDNGNESGEE DVKKINIEDL
NXRSXETSES LKLSPVHEFV RRIIPRNFYK DAIINQTCLI LNSKDPNFQE TSLIVYTPHI
NSEKDCPFYI PRTQSVGILL HQSVLSVHYI PFPEDKTAFT DESERVVRTA YRLLQTANKH
SKGVMQGYEK RVNHDQVVNK VNFQNTYIVL KKKYSKFLVE NWAESTDPKK HVFEDIAIAA
FLIELWIKVY GPDFRSKMQF RDLGCGNGAL CYILLSESIK GLGIDARKRK SWSIYPPEVQ
SSLKEQVIIP SILLRPHPAL KRQVPHLEHN GRFFPVKVTH EVIAPATVVY SSEDLLKSPQ
VNTAEFPPDT FIIGNHSDEL TCWIPLLGHP YMVIPCCSHN FSGQRVRFNV RKRSPRSNEI
KNQNNSKSTY SGLVDHVEYI SSRVGWKVEK EMLRIPSTRN AAIIGVENAT LKHFPTQAVY
DMIWEDGGAE GWIQNTMSLL KRNPRNH
//
