ID H0PZ66_9RHOO Unreviewed; 735 AA.
AC H0PZ66;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 58.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN Name=uvrD {ECO:0000313|EMBL:BAL23603.1};
GN ORFNames=AZKH_1276 {ECO:0000313|EMBL:BAL23603.1};
OS Azoarcus sp. KH32C.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC Azoarcus.
OX NCBI_TaxID=748247 {ECO:0000313|EMBL:BAL23603.1, ECO:0000313|Proteomes:UP000007106};
RN [1] {ECO:0000313|EMBL:BAL23603.1, ECO:0000313|Proteomes:UP000007106}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KH32C {ECO:0000313|EMBL:BAL23603.1,
RC ECO:0000313|Proteomes:UP000007106};
RA Nishizawa T., Tago T., Oshima K., Hattori M., Ishii S., Otsuka S.,
RA Senoo K.;
RT "Complete genome sequence of Azoarcus sp. KH32C.";
RL Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; AP012304; BAL23603.1; -; Genomic_DNA.
DR RefSeq; WP_015434910.1; NC_020516.1.
DR AlphaFoldDB; H0PZ66; -.
DR STRING; 748247.AZKH_1276; -.
DR KEGG; aza:AZKH_1276; -.
DR PATRIC; fig|748247.4.peg.1261; -.
DR eggNOG; COG0210; Bacteria.
DR HOGENOM; CLU_004585_5_5_4; -.
DR OrthoDB; 5905204at2; -.
DR Proteomes; UP000007106; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000007106}.
FT DOMAIN 6..291
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 292..567
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 647..679
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 27..34
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 735 AA; 82475 MW; 2DF67B442B952981 CRC64;
MSDLLANLNA EQLQAVTLPP RHALILAGAG SGKTRVLTTR IAWLVQTGQV DPQGVLAVTF
TNKAAKELLA RLSAMLPLNF GHTARGMWIG TFHGLCNRLL RTHHRDAGLP QLFQILDSGD
QLAAIKRLLK TLNVDDEKFP PRELQHFINA QKEAGLRPHA VQAWDDYTRR RVELYIEYEA
QCQRESVVDF AELLLRSYEL LERNEPIRRH YQRRFRHILV DEFQDTNRLQ YRWLKLLADE
GREGGACLFC VGDDDQSIYR FRGAEVGNMR DFEREFRVDS VIRLEQNYRS HGNILDAANA
IIRHNTGRLG KNLWTERGAG EPIRVFEAYA DSDEARWIVE EIQSLVRDGA SRSDIALLYR
SNAQSRVLEH QLFSSGIAYR VYGGLRFFER QEIKHALAYL RLIANPDDDT AFSRVVNFPT
RGIGARSLET LQDAARTWNT SLYATVPHLT GKPGTVLAQF VRLVEDMRRD TANLPLPELV
DHALDASGLR NFYRSEKEGQ ERLENLDELI NAAANFLADA SPDAEAMAQS HALLADFLAH
ASLEAGDHQA DPGSEAVQLM TVHASKGLEF DVVFLSGLEE GLFPHENSAM ETEGLEEERR
LMYVAVTRAR QRLYLSFAQS RMLHGQTRYN MRSRFLEEIP QALTKWLSPP SPRGMGMRGG
GEPARGGYSP RPAPAPARNQ DLGGLRIGQL VTHAKFGQGV IVAAEGSGSD ARVQINFGSA
GMKWLMLAVA RLTPA
//
