ID H0TIM2_9BRAD Unreviewed; 392 AA.
AC H0TIM2;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE SubName: Full=Acetyl-CoA acetyltransferase with thiolase domain (Acetoacetyl-CoA thiolase) {ECO:0000313|EMBL:CCE06310.1};
DE EC=2.3.1.9 {ECO:0000313|EMBL:CCE06310.1};
GN Name=phbA {ECO:0000313|EMBL:CCE06310.1};
GN ORFNames=BRAS3843_1670017 {ECO:0000313|EMBL:CCE06310.1};
OS Bradyrhizobium sp. STM 3843.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Bradyrhizobium.
OX NCBI_TaxID=551947 {ECO:0000313|EMBL:CCE06310.1, ECO:0000313|Proteomes:UP000002686};
RN [1] {ECO:0000313|Proteomes:UP000002686}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=STM3843 {ECO:0000313|Proteomes:UP000002686};
RX PubMed=24704842; DOI=10.3390/genes3010035;
RA Mornico D., Miche L., Bena G., Nouwen N., Vermeglio A., Vallenet D.,
RA Smith A.A., Giraud E., Medigue C., Moulin L.;
RT "Comparative genomics of aeschynomene symbionts: insights into the
RT ecological lifestyle of nod-independent photosynthetic bradyrhizobia.";
RL Genes (Basel) 3:35-61(2011).
CC -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC {ECO:0000256|ARBA:ARBA00010982, ECO:0000256|RuleBase:RU003557}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCE06310.1}.
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DR EMBL; CAFK01000076; CCE06310.1; -; Genomic_DNA.
DR RefSeq; WP_008968832.1; NZ_CAFK01000076.1.
DR AlphaFoldDB; H0TIM2; -.
DR OrthoDB; 9764638at2; -.
DR Proteomes; UP000002686; Unassembled WGS sequence.
DR GO; GO:0003985; F:acetyl-CoA C-acetyltransferase activity; IEA:UniProtKB-EC.
DR CDD; cd00751; thiolase; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR InterPro; IPR002155; Thiolase.
DR InterPro; IPR016039; Thiolase-like.
DR InterPro; IPR020615; Thiolase_acyl_enz_int_AS.
DR InterPro; IPR020610; Thiolase_AS.
DR InterPro; IPR020617; Thiolase_C.
DR InterPro; IPR020613; Thiolase_CS.
DR InterPro; IPR020616; Thiolase_N.
DR NCBIfam; TIGR01930; AcCoA-C-Actrans; 1.
DR PANTHER; PTHR18919; ACETYL-COA C-ACYLTRANSFERASE; 1.
DR PANTHER; PTHR18919:SF153; TRIFUNCTIONAL ENZYME SUBUNIT BETA, MITOCHONDRIAL; 1.
DR Pfam; PF02803; Thiolase_C; 1.
DR Pfam; PF00108; Thiolase_N; 1.
DR PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR SUPFAM; SSF53901; Thiolase-like; 2.
DR PROSITE; PS00098; THIOLASE_1; 1.
DR PROSITE; PS00737; THIOLASE_2; 1.
DR PROSITE; PS00099; THIOLASE_3; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|RuleBase:RU003557,
KW ECO:0000313|EMBL:CCE06310.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000002686};
KW Transferase {ECO:0000256|RuleBase:RU003557, ECO:0000313|EMBL:CCE06310.1}.
FT DOMAIN 5..261
FT /note="Thiolase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00108"
FT DOMAIN 270..390
FT /note="Thiolase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02803"
FT ACT_SITE 89
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT ACT_SITE 348
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
FT ACT_SITE 378
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000429-1"
SQ SEQUENCE 392 AA; 40579 MW; 3391AF5C1D87CB0D CRC64;
MSDDVVIVSA ARTPVGSFNG AFANLPAHEL GAIAIKAALE RAGVEPGRVS EVIMGQILTA
AQGQNPARQA SIAAGIPVES PAWGVNQLCG SGLRTVALGY QALLNGDSEI VVAGGQESMS
MATHAQHLRG GVKMGSLELV DTMIKDGLWD AFNGYHMGNT AENVAKQYQI TRAQQDEFAV
ASQNKAEAAQ KAGRFKDEIV PVTIKSRKGD VVVDADEYPR HGATMDAMGK LRPAFEKDGT
VTAGNASGIN DGAAAVVLMT AKQAAKEGRT PIARIVSWGQ AGVDPKIMGT GPIPASRTAL
KKAGWNIADL DLIEANEAFA AQACAVNKDL GWDPAKVNVN GGAIAIGHPI GASGARVLVT
LLHEMQKRDA KKGLATLCIG GGMGIAMCIA RD
//
