ID H0VCS4_CAVPO Unreviewed; 316 AA.
AC H0VCS4;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 2.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=tRNA methyltransferase 10 homolog B {ECO:0000256|ARBA:ARBA00035688};
DE EC=2.1.1.221 {ECO:0000256|ARBA:ARBA00012797};
DE AltName: Full=RNA (guanine-9-)-methyltransferase domain-containing protein 3 {ECO:0000256|ARBA:ARBA00035712};
DE AltName: Full=tRNA (guanine(9)-N(1))-methyltransferase TRMT10B {ECO:0000256|ARBA:ARBA00035725};
GN Name=TRMT10B {ECO:0000313|Ensembl:ENSCPOP00000007752.3};
OS Cavia porcellus (Guinea pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Caviidae;
OC Cavia.
OX NCBI_TaxID=10141 {ECO:0000313|Ensembl:ENSCPOP00000007752.3, ECO:0000313|Proteomes:UP000005447};
RN [1] {ECO:0000313|Proteomes:UP000005447}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2N {ECO:0000313|Proteomes:UP000005447};
RX PubMed=21993624; DOI=10.1038/nature10530;
RA Lindblad-Toh K., Garber M., Zuk O., Lin M.F., Parker B.J., Washietl S.,
RA Kheradpour P., Ernst J., Jordan G., Mauceli E., Ward L.D., Lowe C.B.,
RA Holloway A.K., Clamp M., Gnerre S., Alfoldi J., Beal K., Chang J.,
RA Clawson H., Cuff J., Di Palma F., Fitzgerald S., Flicek P., Guttman M.,
RA Hubisz M.J., Jaffe D.B., Jungreis I., Kent W.J., Kostka D., Lara M.,
RA Martins A.L., Massingham T., Moltke I., Raney B.J., Rasmussen M.D.,
RA Robinson J., Stark A., Vilella A.J., Wen J., Xie X., Zody M.C., Baldwin J.,
RA Bloom T., Chin C.W., Heiman D., Nicol R., Nusbaum C., Young S.,
RA Wilkinson J., Worley K.C., Kovar C.L., Muzny D.M., Gibbs R.A., Cree A.,
RA Dihn H.H., Fowler G., Jhangiani S., Joshi V., Lee S., Lewis L.R.,
RA Nazareth L.V., Okwuonu G., Santibanez J., Warren W.C., Mardis E.R.,
RA Weinstock G.M., Wilson R.K., Delehaunty K., Dooling D., Fronik C.,
RA Fulton L., Fulton B., Graves T., Minx P., Sodergren E., Birney E.,
RA Margulies E.H., Herrero J., Green E.D., Haussler D., Siepel A., Goldman N.,
RA Pollard K.S., Pedersen J.S., Lander E.S., Kellis M.;
RT "A high-resolution map of human evolutionary constraint using 29 mammals.";
RL Nature 478:476-482(2011).
RN [2] {ECO:0000313|Ensembl:ENSCPOP00000007752.3}
RP IDENTIFICATION.
RC STRAIN=2N {ECO:0000313|Ensembl:ENSCPOP00000007752.3};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(9) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-
CC methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43156, Rhea:RHEA-COMP:10367, Rhea:RHEA-COMP:10368,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.221;
CC Evidence={ECO:0000256|ARBA:ARBA00000855};
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DR EMBL; AAKN02030467; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_003470758.1; XM_003470710.3.
DR RefSeq; XP_005004163.1; XM_005004106.2.
DR RefSeq; XP_013008977.1; XM_013153523.1.
DR AlphaFoldDB; H0VCS4; -.
DR STRING; 10141.ENSCPOP00000007752; -.
DR Ensembl; ENSCPOT00000008707.3; ENSCPOP00000007752.3; ENSCPOG00000008629.4.
DR GeneID; 100733884; -.
DR KEGG; cpoc:100733884; -.
DR CTD; 158234; -.
DR VEuPathDB; HostDB:ENSCPOG00000008629; -.
DR eggNOG; KOG2967; Eukaryota.
DR GeneTree; ENSGT00530000063169; -.
DR HOGENOM; CLU_034384_8_0_1; -.
DR InParanoid; H0VCS4; -.
DR OMA; ALQAWFP; -.
DR OrthoDB; 2908056at2759; -.
DR TreeFam; TF330972; -.
DR Proteomes; UP000005447; Unassembled WGS sequence.
DR Bgee; ENSCPOG00000008629; Expressed in pituitary gland and 13 other cell types or tissues.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IEA:Ensembl.
DR GO; GO:0052905; F:tRNA (guanosine(9)-N1)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IEA:Ensembl.
DR CDD; cd18100; Trm10euk_B; 1.
DR Gene3D; 3.40.1280.30; -; 1.
DR InterPro; IPR028564; MT_TRM10-typ.
DR InterPro; IPR038459; MT_TRM10-typ_sf.
DR InterPro; IPR047911; Trm10_B_MTase_dom.
DR InterPro; IPR007356; tRNA_m1G_MeTrfase_euk.
DR InterPro; IPR016009; tRNA_MeTrfase_TRMD/TRM10.
DR PANTHER; PTHR13563; TRNA (GUANINE-9-) METHYLTRANSFERASE; 1.
DR PANTHER; PTHR13563:SF19; TRNA METHYLTRANSFERASE 10 HOMOLOG B; 1.
DR Pfam; PF01746; tRNA_m1G_MT; 1.
DR PROSITE; PS51675; SAM_MT_TRM10; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000005447};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 113..310
FT /note="SAM-dependent MTase TRM10-type"
FT /evidence="ECO:0000259|PROSITE:PS51675"
FT REGION 1..31
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 78..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 316 AA; 36174 MW; B3CEEDC23B33EA79 CRC64;
MDWKLKGSTQ RTESPMLQGQ EGGAEDTEAD ELSESFQLLQ IDVACECQER ETLPTGGTPW
SSKNVQRKQR HWEKIIAARK SKRKQEKERK KANRAENPGV CTQHSKRFLK ALTKEKLLEA
RHSGPRLCID LSMTDHMSKK EMSRLAGQIR RLYGSNKRAD RPFWICLTGF TAASPLYEEC
LRMNDGFSSY QLDITEEDCF SLFPLESLVY LTPDSEHALE DVDLNKVYVL GGLVDESIQK
KVTFQKAQEH SVKTARLPIQ EYMVRRPNGK NYHSEILAIN QVFDILSTYF DTHNWPKALK
KGVSPGKGYV LQSPDE
//
