ID H0VDF2_CAVPO Unreviewed; 1566 AA.
AC H0VDF2;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 2.
DT 27-MAR-2024, entry version 75.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN Name=HECW2 {ECO:0000313|Ensembl:ENSCPOP00000008002.3};
OS Cavia porcellus (Guinea pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Caviidae;
OC Cavia.
OX NCBI_TaxID=10141 {ECO:0000313|Ensembl:ENSCPOP00000008002.3, ECO:0000313|Proteomes:UP000005447};
RN [1] {ECO:0000313|Proteomes:UP000005447}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2N {ECO:0000313|Proteomes:UP000005447};
RX PubMed=21993624; DOI=10.1038/nature10530;
RA Lindblad-Toh K., Garber M., Zuk O., Lin M.F., Parker B.J., Washietl S.,
RA Kheradpour P., Ernst J., Jordan G., Mauceli E., Ward L.D., Lowe C.B.,
RA Holloway A.K., Clamp M., Gnerre S., Alfoldi J., Beal K., Chang J.,
RA Clawson H., Cuff J., Di Palma F., Fitzgerald S., Flicek P., Guttman M.,
RA Hubisz M.J., Jaffe D.B., Jungreis I., Kent W.J., Kostka D., Lara M.,
RA Martins A.L., Massingham T., Moltke I., Raney B.J., Rasmussen M.D.,
RA Robinson J., Stark A., Vilella A.J., Wen J., Xie X., Zody M.C., Baldwin J.,
RA Bloom T., Chin C.W., Heiman D., Nicol R., Nusbaum C., Young S.,
RA Wilkinson J., Worley K.C., Kovar C.L., Muzny D.M., Gibbs R.A., Cree A.,
RA Dihn H.H., Fowler G., Jhangiani S., Joshi V., Lee S., Lewis L.R.,
RA Nazareth L.V., Okwuonu G., Santibanez J., Warren W.C., Mardis E.R.,
RA Weinstock G.M., Wilson R.K., Delehaunty K., Dooling D., Fronik C.,
RA Fulton L., Fulton B., Graves T., Minx P., Sodergren E., Birney E.,
RA Margulies E.H., Herrero J., Green E.D., Haussler D., Siepel A., Goldman N.,
RA Pollard K.S., Pedersen J.S., Lander E.S., Kellis M.;
RT "A high-resolution map of human evolutionary constraint using 29 mammals.";
RL Nature 478:476-482(2011).
RN [2] {ECO:0000313|Ensembl:ENSCPOP00000008002.3}
RP IDENTIFICATION.
RC STRAIN=2N {ECO:0000313|Ensembl:ENSCPOP00000008002.3};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
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DR EMBL; AAKN02051331; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAKN02051332; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAKN02051333; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAKN02051334; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_003463417.2; XM_003463369.3.
DR STRING; 10141.ENSCPOP00000008002; -.
DR Ensembl; ENSCPOT00000008988.3; ENSCPOP00000008002.3; ENSCPOG00000008908.4.
DR VEuPathDB; HostDB:ENSCPOG00000008908; -.
DR eggNOG; KOG0940; Eukaryota.
DR GeneTree; ENSGT00940000155466; -.
DR HOGENOM; CLU_002173_14_0_1; -.
DR InParanoid; H0VDF2; -.
DR OMA; TAGQHRE; -.
DR OrthoDB; 5480520at2759; -.
DR TreeFam; TF313938; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000005447; Unassembled WGS sequence.
DR Bgee; ENSCPOG00000008908; Expressed in frontal cortex and 10 other cell types or tissues.
DR GO; GO:0072686; C:mitotic spindle; IEA:Ensembl.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0030071; P:regulation of mitotic metaphase/anaphase transition; IEA:Ensembl.
DR CDD; cd08691; C2_NEDL1-like; 1.
DR CDD; cd00078; HECTc; 1.
DR CDD; cd00201; WW; 2.
DR Gene3D; 2.20.70.10; -; 2.
DR Gene3D; 2.60.40.2840; -; 1.
DR Gene3D; 2.60.40.150; C2 domain; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000008; C2_dom.
DR InterPro; IPR035892; C2_domain_sf.
DR InterPro; IPR037795; C2_HECW.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR040524; HECW1_helix.
DR InterPro; IPR032348; HECW_N.
DR InterPro; IPR001202; WW_dom.
DR InterPro; IPR036020; WW_dom_sf.
DR PANTHER; PTHR11254:SF127; E3 UBIQUITIN-PROTEIN LIGASE HECW2; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF00168; C2; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF18436; HECW1_helix; 1.
DR Pfam; PF16562; HECW_N; 1.
DR Pfam; PF00397; WW; 1.
DR SMART; SM00239; C2; 1.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00456; WW; 2.
DR SUPFAM; SSF49562; C2 domain (Calcium/lipid-binding domain, CaLB); 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF51045; WW domain; 2.
DR PROSITE; PS50004; C2; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS01159; WW_DOMAIN_1; 2.
DR PROSITE; PS50020; WW_DOMAIN_2; 2.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000005447};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 171..301
FT /note="C2"
FT /evidence="ECO:0000259|PROSITE:PS50004"
FT DOMAIN 801..834
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT DOMAIN 979..1012
FT /note="WW"
FT /evidence="ECO:0000259|PROSITE:PS50020"
FT DOMAIN 1231..1566
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 342..453
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 483..725
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 769..788
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1010..1063
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1155..1181
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 366..413
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 510..536
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 562..626
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 640..664
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1010..1027
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1157..1175
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 1534
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 1566 AA; 174919 MW; C9EAF7E0505B181B CRC64;
MASSAREHLL FVRRRNPQMR YTLSPENLQS LAAQGSLPEN MTLPRANSDT DLVTSESRSS
LTASMYEYTL GQAQNLIIFW DIKEEVDPSD WIGLYHIDEN SPANFWDSKN RGVTGTQKGQ
IVWRIEPGPY FMEPEIKICF KYYHGISGAL RATTPCITVK NPAVMMGAEG TEGGMSGSLH
SRKLVSFTLS DLRAVGLKKG MFFNPDPYLK MSIQPGKKSS FPTCAHHGQE RRSTIISNTT
NPIWHREKYS FFALLTDVLE IEIKDKFAKS RPIIKRFLGK LTIPVQRLLE RQAIGDQMLS
YNLGRRLPAD HVSGYLQFKV EVTSSAHEDA SPEAVGTILG VSTVNGDLGS PSDDEDMPGS
HQDSPGCANG AVSEDSTADG TPKHSFRTSS TLEMDAEELT STSSRTSPPR GRQDSLNDYL
DALEHDGPAR PGATASERPM GASPKLRSSF PTDTRLSAML HIDSDEEDHE LQQDLGYPSS
LEEEGGLIML SRPSRADEGS LTSQTKSEDT NLVDLEEHSV LEAVSQEDKP EDLPELADGP
LDAAPEDGEA AGPDSHPEVE QGSTKLCGSQ EAEQPASGAD TGTPDTSGGS RRAISETESL
DQGSEPSQVS SETEPSDPAR TESVSEASTR PEGESDLEGA DSSCNDSVAT QLSSLESARP
PGTPAFSSQE DEDGACAAAA AEPPSRGPGP EPACTPGTGM LPAVQVPEAQ EDAGPEPKDL
GEIWQRRAGL EGASAIENES RSQALEEEEA QEDMGEDARA CEGAAALEEG AAGGSPANGH
QPLRSLPSVR QDVSRYQRVD EALPPNWEAR IDSHGRIFYV DHVNRTTTWQ RPTAPPAPQV
LQRSNSIQQM EQLNRRYQSI RRTMTNERPE ESASAMDGAG EEVDFHQTSA DFRRESVLPH
STSRSRLALL LQSPPVKFLI SPEFFTVLHS NPSAYRMFTN NTCLKHMITK VRRDTHHFER
YQHNRDLVGF LNMFANKQLE LPRGWEMKHD HQGKAFFVDH NSRTTTFIDP RLPLQSSRPT
SALAHRQHLT RQRSHSAGEV GEDSRHAGPP VLPRPSSTFN TVNRPQYQDM VPVAYNDKIV
AFLRQPNIFE ILQERQPDLT RNHSLREKIQ FIRTEGTPGL VRLSSDADLV MLLSLFEEEI
MSYVPPHALL HPSYCQSPRG SPVSSPQNSP GTQRANARAP APYKRDFEAK LRNFYRKLET
KGYGQGPGKL KLIIRRDHLL EDAFNQIMGY SRKDLQRNKL YVTFVGEEGL DYSGPSREFF
FLVSRELFNP YYGLFEYSAN DTYTVQISPM SAFVDNHHEW FRFSGRILGL ALIHQYLLDA
FFTRPFYKAL LRILCDLSDL EYLDEEFHQS LQWMKDNDIH DILDLTFTVN EEVFGQITER
ELKPGGANIP VTEKNKKEYI ERMVKWRIER GVVQQTESLV RGFYEVVDAR LVSVFDAREL
ELVIAGTAEI DLSDWRSNTE YRGGYHDNHI VIRWFWAAVE RFNNEQRLRL LQFVTGTSSI
PYEGFASLRG SNGPRRFCVE KWGKITALPR AHTCFNRLDL PPYPSFSMLY EKLLTAVEET
STFGLE
//
