ID H0VK70_CAVPO Unreviewed; 395 AA.
AC H0VK70;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 62.
DE RecName: Full=GPI-anchor transamidase {ECO:0000256|ARBA:ARBA00019393};
DE AltName: Full=Phosphatidylinositol-glycan biosynthesis class K protein {ECO:0000256|ARBA:ARBA00029842};
GN Name=PIGK {ECO:0000313|Ensembl:ENSCPOP00000010680.2};
OS Cavia porcellus (Guinea pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Caviidae;
OC Cavia.
OX NCBI_TaxID=10141 {ECO:0000313|Ensembl:ENSCPOP00000010680.2, ECO:0000313|Proteomes:UP000005447};
RN [1] {ECO:0000313|Proteomes:UP000005447}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2N {ECO:0000313|Proteomes:UP000005447};
RX PubMed=21993624; DOI=10.1038/nature10530;
RA Lindblad-Toh K., Garber M., Zuk O., Lin M.F., Parker B.J., Washietl S.,
RA Kheradpour P., Ernst J., Jordan G., Mauceli E., Ward L.D., Lowe C.B.,
RA Holloway A.K., Clamp M., Gnerre S., Alfoldi J., Beal K., Chang J.,
RA Clawson H., Cuff J., Di Palma F., Fitzgerald S., Flicek P., Guttman M.,
RA Hubisz M.J., Jaffe D.B., Jungreis I., Kent W.J., Kostka D., Lara M.,
RA Martins A.L., Massingham T., Moltke I., Raney B.J., Rasmussen M.D.,
RA Robinson J., Stark A., Vilella A.J., Wen J., Xie X., Zody M.C., Baldwin J.,
RA Bloom T., Chin C.W., Heiman D., Nicol R., Nusbaum C., Young S.,
RA Wilkinson J., Worley K.C., Kovar C.L., Muzny D.M., Gibbs R.A., Cree A.,
RA Dihn H.H., Fowler G., Jhangiani S., Joshi V., Lee S., Lewis L.R.,
RA Nazareth L.V., Okwuonu G., Santibanez J., Warren W.C., Mardis E.R.,
RA Weinstock G.M., Wilson R.K., Delehaunty K., Dooling D., Fronik C.,
RA Fulton L., Fulton B., Graves T., Minx P., Sodergren E., Birney E.,
RA Margulies E.H., Herrero J., Green E.D., Haussler D., Siepel A., Goldman N.,
RA Pollard K.S., Pedersen J.S., Lander E.S., Kellis M.;
RT "A high-resolution map of human evolutionary constraint using 29 mammals.";
RL Nature 478:476-482(2011).
RN [2] {ECO:0000313|Ensembl:ENSCPOP00000010680.2}
RP IDENTIFICATION.
RC STRAIN=2N {ECO:0000313|Ensembl:ENSCPOP00000010680.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- PATHWAY: Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor
CC biosynthesis. {ECO:0000256|ARBA:ARBA00004687}.
CC -!- SUBUNIT: Forms a complex with PIGT, PIGS, PIGU and GAA1.
CC {ECO:0000256|ARBA:ARBA00011178}.
CC -!- SIMILARITY: Belongs to the peptidase C13 family.
CC {ECO:0000256|ARBA:ARBA00009941}.
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DR EMBL; AAKN02005004; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAKN02005005; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_003478859.1; XM_003478811.3.
DR AlphaFoldDB; H0VK70; -.
DR STRING; 10141.ENSCPOP00000010680; -.
DR MEROPS; C13.005; -.
DR Ensembl; ENSCPOT00000011989.3; ENSCPOP00000010680.2; ENSCPOG00000011874.4.
DR GeneID; 100715468; -.
DR KEGG; cpoc:100715468; -.
DR CTD; 10026; -.
DR VEuPathDB; HostDB:ENSCPOG00000011874; -.
DR eggNOG; KOG1349; Eukaryota.
DR GeneTree; ENSGT00940000156273; -.
DR HOGENOM; CLU_044656_1_0_1; -.
DR InParanoid; H0VK70; -.
DR OMA; PGHTNNW; -.
DR OrthoDB; 1122658at2759; -.
DR TreeFam; TF300848; -.
DR UniPathway; UPA00196; -.
DR Proteomes; UP000005447; Unassembled WGS sequence.
DR Bgee; ENSCPOG00000011874; Expressed in heart and 13 other cell types or tissues.
DR GO; GO:0042765; C:GPI-anchor transamidase complex; IEA:Ensembl.
DR GO; GO:0003923; F:GPI-anchor transamidase activity; IEA:Ensembl.
DR GO; GO:0016255; P:attachment of GPI anchor to protein; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1460; -; 1.
DR InterPro; IPR028361; GPI_transamidase.
DR InterPro; IPR001096; Peptidase_C13.
DR PANTHER; PTHR48067; GPI-ANCHOR TRANSAMIDASE; 1.
DR PANTHER; PTHR48067:SF1; GPI-ANCHOR TRANSAMIDASE; 1.
DR Pfam; PF01650; Peptidase_C13; 1.
DR PIRSF; PIRSF500138; GPI8; 1.
DR PIRSF; PIRSF019663; Legumain; 1.
DR PRINTS; PR00776; HEMOGLOBNASE.
PE 3: Inferred from homology;
KW GPI-anchor biosynthesis {ECO:0000256|ARBA:ARBA00022502};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000005447};
KW Signal {ECO:0000256|SAM:SignalP}; Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..28
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 29..395
FT /note="GPI-anchor transamidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5027773848"
FT TRANSMEM 364..383
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT ACT_SITE 164
FT /evidence="ECO:0000256|PIRSR:PIRSR019663-1"
FT ACT_SITE 206
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR019663-1"
SQ SEQUENCE 395 AA; 45384 MW; 6593FB12536B610A CRC64;
MAATYVLKHS ATTLAAFLFL SFVSSAASHI EDQAEQFFRS GHTNNWAVLV CTSRFWFNYR
HVANTLSVYR SVKRLGIPDS HIVLMLADDM ACNPRNPKPA TVFSHKNMEL NVYGDDVEVD
YRSYEVTVEN FLRVLTGRIP PSTPRSKRLL SDDRSNILIY MTGHGGNGFL KFQDSEEITN
IELADAFEQM WQKRRYNELL FIIDTCQGAS MYERFYSPNI MALASSQVGE DSLSHQPDPA
IGVHLMDRYT FYVLEFLEEI NPASQTNMND LFQVCPKSLC VSTPGHRTDL FKRDPKNVLI
TDFFGSVRKV EITTETMSLQ QDSEIMESSY KEDQMDEELM EPLKYAEQLP VAQIIHQKPK
LKDWHPPGGF ILGLWALIIM VFFKTYGIKH MKFIF
//
