ID H0WHL8_OTOGA Unreviewed; 408 AA.
AC H0WHL8;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 57.
DE RecName: Full=Neutral cholesterol ester hydrolase 1 {ECO:0000256|ARBA:ARBA00044162};
DE EC=3.1.1.71 {ECO:0000256|ARBA:ARBA00044060};
DE AltName: Full=Acetylalkylglycerol acetylhydrolase {ECO:0000256|ARBA:ARBA00044219};
DE AltName: Full=Arylacetamide deacetylase-like 1 {ECO:0000256|ARBA:ARBA00044256};
GN Name=NCEH1 {ECO:0000313|Ensembl:ENSOGAP00000000872.2};
OS Otolemur garnettii (Small-eared galago) (Garnett's greater bushbaby).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Strepsirrhini; Lorisiformes;
OC Galagidae; Otolemur.
OX NCBI_TaxID=30611 {ECO:0000313|Ensembl:ENSOGAP00000000872.2, ECO:0000313|Proteomes:UP000005225};
RN [1] {ECO:0000313|Proteomes:UP000005225}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG The Broad Institute Genome Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K., Jaffe D.B.,
RA Gnerre S., MacCallum I., Przybylski D., Ribeiro F.J., Burton J.N.,
RA Walker B.J., Sharpe T., Hall G.;
RT "Version 3 of the genome sequence of Otolemur garnettii (Bushbaby).";
RL Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSOGAP00000000872.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-O-hexadecyl-2-acetyl-sn-glycerol + H2O = 1-O-hexadecyl-sn-
CC glycerol + acetate + H(+); Xref=Rhea:RHEA:38563, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30089, ChEBI:CHEBI:34115,
CC ChEBI:CHEBI:75936; Evidence={ECO:0000256|ARBA:ARBA00023406};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38564;
CC Evidence={ECO:0000256|ARBA:ARBA00023406};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1-O-alkyl-2-acetyl-sn-glycerol + H2O = 1-O-alkyl-sn-glycerol
CC + acetate + H(+); Xref=Rhea:RHEA:11552, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15850, ChEBI:CHEBI:16291,
CC ChEBI:CHEBI:30089; EC=3.1.1.71;
CC Evidence={ECO:0000256|ARBA:ARBA00043796};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11553;
CC Evidence={ECO:0000256|ARBA:ARBA00043796};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a cholesterol ester + H2O = a fatty acid + cholesterol + H(+);
CC Xref=Rhea:RHEA:36403, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16113, ChEBI:CHEBI:17002, ChEBI:CHEBI:28868;
CC Evidence={ECO:0000256|ARBA:ARBA00043699};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:36404;
CC Evidence={ECO:0000256|ARBA:ARBA00043699};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=cholesteryl (9Z-octadecenoate) + H2O = (9Z)-octadecenoate +
CC cholesterol + H(+); Xref=Rhea:RHEA:33875, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:16113, ChEBI:CHEBI:30823,
CC ChEBI:CHEBI:46898; Evidence={ECO:0000256|ARBA:ARBA00023350};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33876;
CC Evidence={ECO:0000256|ARBA:ARBA00023350};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004401};
CC Single-pass type II membrane protein {ECO:0000256|ARBA:ARBA00004401}.
CC Membrane {ECO:0000256|ARBA:ARBA00004606}; Single-pass type II membrane
CC protein {ECO:0000256|ARBA:ARBA00004606}. Microsome
CC {ECO:0000256|ARBA:ARBA00004144}.
CC -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family.
CC {ECO:0000256|ARBA:ARBA00010515}.
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DR EMBL; AAQR03109734; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03109735; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03109736; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03109737; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03109738; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03109739; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_012663374.1; XM_012807920.1.
DR AlphaFoldDB; H0WHL8; -.
DR STRING; 30611.ENSOGAP00000000872; -.
DR ESTHER; otoga-h0whl8; Arylacetamide_deacetylase.
DR Ensembl; ENSOGAT00000000977.2; ENSOGAP00000000872.2; ENSOGAG00000000976.2.
DR GeneID; 100965102; -.
DR KEGG; oga:100965102; -.
DR CTD; 57552; -.
DR eggNOG; KOG1515; Eukaryota.
DR GeneTree; ENSGT00940000156699; -.
DR HOGENOM; CLU_012494_12_0_1; -.
DR InParanoid; H0WHL8; -.
DR OMA; DSWKLML; -.
DR OrthoDB; 1144477at2759; -.
DR TreeFam; TF314978; -.
DR Proteomes; UP000005225; Unassembled WGS sequence.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0052689; F:carboxylic ester hydrolase activity; IEA:InterPro.
DR GO; GO:0042301; F:phosphate ion binding; IEA:Ensembl.
DR GO; GO:0017171; F:serine hydrolase activity; IEA:Ensembl.
DR GO; GO:0046485; P:ether lipid metabolic process; IEA:Ensembl.
DR GO; GO:0006805; P:xenobiotic metabolic process; IEA:Ensembl.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR013094; AB_hydrolase_3.
DR InterPro; IPR017157; Arylacetamide_deacetylase.
DR InterPro; IPR033140; Lipase_GDXG_put_SER_AS.
DR PANTHER; PTHR48081; AB HYDROLASE SUPERFAMILY PROTEIN C4A8.06C; 1.
DR PANTHER; PTHR48081:SF33; SIMILAR TO HYPOTHETICAL PROTEIN C130079G13; 1.
DR Pfam; PF07859; Abhydrolase_3; 2.
DR PIRSF; PIRSF037251; Arylacetamide_deacetylase; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR PROSITE; PS01174; LIPASE_GDXG_SER; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022848};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022963};
KW Membrane {ECO:0000256|ARBA:ARBA00022989};
KW Microsome {ECO:0000256|ARBA:ARBA00022848};
KW Reference proteome {ECO:0000313|Proteomes:UP000005225};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989}.
FT DOMAIN 109..263
FT /note="Alpha/beta hydrolase fold-3"
FT /evidence="ECO:0000259|Pfam:PF07859"
FT DOMAIN 320..381
FT /note="Alpha/beta hydrolase fold-3"
FT /evidence="ECO:0000259|Pfam:PF07859"
FT ACT_SITE 191
FT /evidence="ECO:0000256|PIRSR:PIRSR037251-1,
FT ECO:0000256|PROSITE-ProRule:PRU10038"
FT ACT_SITE 348
FT /evidence="ECO:0000256|PIRSR:PIRSR037251-1"
FT ACT_SITE 378
FT /evidence="ECO:0000256|PIRSR:PIRSR037251-1"
SQ SEQUENCE 408 AA; 45928 MW; A61BCA746A6D3466 CRC64;
MRASCVLLAA LAALAAYYVY IPLPGSVSDP WKLMLLDATF RGAQQVSNLI HYLGLSHHLL
ALNFIIVSFG KKTAWSSAQV KVTDTEFDGV EVRVFEGPRK PEEPLQRSVV YVHGGGWALA
SAKIRYYDEL CTVMAEELNA VIVSIEYRLV PKVYFPEQIH DVVRATKHFL QPEILQKYMV
DPGRICISGD SAGGNLAAAL AQQFNQDANL KNKLKLQALI YPVLQALDFN TPSYQQNVNT
PILPRYVMVK YWVDYFKGSY DFVQAMIVNN HTSLDVEEAT ALRARLNWTS LLPALITKSY
KPVVQATGNP RIVQEIPQLL DARAAPLIAD QEVLRDLPKT YILTCEHDVL RDDGIMYAKR
LESAGVEVTL DHFDDGFHGC MIFTNWPTNF SVGIRTRKSY IEWLDTNL
//
