ID H0WP52_OTOGA Unreviewed; 551 AA.
AC H0WP52;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 60.
DE SubName: Full=5'-nucleotidase domain containing 2 {ECO:0000313|Ensembl:ENSOGAP00000003642.2};
GN Name=NT5DC2 {ECO:0000313|Ensembl:ENSOGAP00000003642.2};
OS Otolemur garnettii (Small-eared galago) (Garnett's greater bushbaby).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Strepsirrhini; Lorisiformes;
OC Galagidae; Otolemur.
OX NCBI_TaxID=30611 {ECO:0000313|Ensembl:ENSOGAP00000003642.2, ECO:0000313|Proteomes:UP000005225};
RN [1] {ECO:0000313|Proteomes:UP000005225}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG The Broad Institute Genome Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K., Jaffe D.B.,
RA Gnerre S., MacCallum I., Przybylski D., Ribeiro F.J., Burton J.N.,
RA Walker B.J., Sharpe T., Hall G.;
RT "Version 3 of the genome sequence of Otolemur garnettii (Bushbaby).";
RL Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSOGAP00000003642.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR017434-2};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR017434-2};
CC -!- SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family.
CC {ECO:0000256|ARBA:ARBA00009589}.
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DR EMBL; AAQR03061738; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03061739; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_003789424.1; XM_003789376.2.
DR AlphaFoldDB; H0WP52; -.
DR STRING; 30611.ENSOGAP00000003642; -.
DR Ensembl; ENSOGAT00000004089.2; ENSOGAP00000003642.2; ENSOGAG00000004086.2.
DR GeneID; 100948616; -.
DR KEGG; oga:100948616; -.
DR CTD; 64943; -.
DR eggNOG; KOG2470; Eukaryota.
DR GeneTree; ENSGT00940000159940; -.
DR HOGENOM; CLU_017845_5_1_1; -.
DR InParanoid; H0WP52; -.
DR OMA; WARYHEM; -.
DR OrthoDB; 3626840at2759; -.
DR TreeFam; TF323990; -.
DR Proteomes; UP000005225; Unassembled WGS sequence.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd07522; HAD_cN-II; 1.
DR Gene3D; 3.40.50.1000; HAD superfamily/HAD-like; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR008380; HAD-SF_hydro_IG_5-nucl.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR016695; Pur_nucleotidase.
DR NCBIfam; TIGR02244; HAD-IG-Ncltidse; 1.
DR PANTHER; PTHR12103; 5'-NUCLEOTIDASE DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR12103:SF14; 5'-NUCLEOTIDASE DOMAIN-CONTAINING PROTEIN 2; 1.
DR Pfam; PF05761; 5_nucleotid; 1.
DR PIRSF; PIRSF017434; Purine_5'-nucleotidase; 1.
DR SUPFAM; SSF56784; HAD-like; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|PIRSR:PIRSR017434-2};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR017434-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000005225}.
FT ACT_SITE 104
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-1"
FT ACT_SITE 106
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-1"
FT BINDING 104
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
FT BINDING 106
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
FT BINDING 389
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
SQ SEQUENCE 551 AA; 63727 MW; 4930BBE5D66D8539 CRC64;
MAGAGLRAAA WRWLLCGGQG GPRAASSSCP GCGPPGPGAH CPGAPRSAPA GSTEVNSHLW
SRYQDMRRLV HDLLPPEVCS LLNPAAIYAN NEISLRDVEV YGFDYDYTLA QYADILHPEI
FSAARDILIE HYKYPEGIRK YDYNPNFAIR GLHYDIQKSL LMKIDAFHYV QLGTAYRGLQ
PVPDEEVIDL YGGTQHIPLY QMSGFYGKGP SIKQFMDIFS LPEMALLSCV VDYFLGHSLE
FDQAHLYKDV TDAIRDVHVK GLMYQWIEQD MEKYILRGDE TFAVLNRLVT HGKQLFLITN
SPFSFVDKGM RHMVGPDWRQ LFDVVIVQAD KPSFFTDRRK PFRKLDEKGS LQWDRITRLE
KGKIYRQGNL FDFLRLTEWR GPRVLYFGDH LYSDLADLML RHGWRTGAII PELEREIRII
NTEQYMHSLT WQQALTGLLE RMQTYQDAES RQVLAAWMKE RQELRCITKA LFNAQFGSIF
RTFHNPTYFS RRLVRFSDLY MASLSCLLNY RVDFTFYPRR TPLQHEAPLW MDQLCTGCMK
TPFLGDMAHI R
//
