ID H0WSN6_OTOGA Unreviewed; 1401 AA.
AC H0WSN6;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 70.
DE RecName: Full=DNA helicase {ECO:0000256|ARBA:ARBA00012551};
DE EC=3.6.4.12 {ECO:0000256|ARBA:ARBA00012551};
GN Name=BLM {ECO:0000313|Ensembl:ENSOGAP00000005104.2};
OS Otolemur garnettii (Small-eared galago) (Garnett's greater bushbaby).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Strepsirrhini; Lorisiformes;
OC Galagidae; Otolemur.
OX NCBI_TaxID=30611 {ECO:0000313|Ensembl:ENSOGAP00000005104.2, ECO:0000313|Proteomes:UP000005225};
RN [1] {ECO:0000313|Proteomes:UP000005225}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG The Broad Institute Genome Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K., Jaffe D.B.,
RA Gnerre S., MacCallum I., Przybylski D., Ribeiro F.J., Burton J.N.,
RA Walker B.J., Sharpe T., Hall G.;
RT "Version 3 of the genome sequence of Otolemur garnettii (Bushbaby).";
RL Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSOGAP00000005104.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12;
CC Evidence={ECO:0000256|ARBA:ARBA00001665};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SIMILARITY: Belongs to the helicase family. RecQ subfamily.
CC {ECO:0000256|ARBA:ARBA00005446}.
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DR EMBL; AAQR03183224; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03183225; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03183226; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03183227; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03183228; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03183229; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03183230; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03183231; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03183232; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03183233; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR STRING; 30611.ENSOGAP00000005104; -.
DR Ensembl; ENSOGAT00000005714.2; ENSOGAP00000005104.2; ENSOGAG00000005708.2.
DR eggNOG; KOG0351; Eukaryota.
DR GeneTree; ENSGT00940000156800; -.
DR HOGENOM; CLU_001103_1_1_1; -.
DR InParanoid; H0WSN6; -.
DR OMA; FHVMEDI; -.
DR TreeFam; TF317801; -.
DR Proteomes; UP000005225; Unassembled WGS sequence.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:Ensembl.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0000800; C:lateral element; IEA:Ensembl.
DR GO; GO:0016363; C:nuclear matrix; IEA:Ensembl.
DR GO; GO:0005730; C:nucleolus; IEA:Ensembl.
DR GO; GO:0016605; C:PML body; IEA:Ensembl.
DR GO; GO:0031422; C:RecQ family helicase-topoisomerase III complex; IEA:Ensembl.
DR GO; GO:0043138; F:3'-5' DNA helicase activity; IEA:InterPro.
DR GO; GO:1905773; F:8-hydroxy-2'-deoxyguanosine DNA binding; IEA:Ensembl.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000405; F:bubble DNA binding; IEA:Ensembl.
DR GO; GO:1990814; F:DNA/DNA annealing activity; IEA:Ensembl.
DR GO; GO:0061749; F:forked DNA-dependent helicase activity; IEA:Ensembl.
DR GO; GO:0000400; F:four-way junction DNA binding; IEA:Ensembl.
DR GO; GO:0009378; F:four-way junction helicase activity; IEA:Ensembl.
DR GO; GO:0016818; F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides; IEA:InterPro.
DR GO; GO:0140677; F:molecular function activator activity; IEA:Ensembl.
DR GO; GO:0002039; F:p53 binding; IEA:Ensembl.
DR GO; GO:0042803; F:protein homodimerization activity; IEA:Ensembl.
DR GO; GO:0061821; F:telomeric D-loop binding; IEA:Ensembl.
DR GO; GO:0061849; F:telomeric G-quadruplex DNA binding; IEA:Ensembl.
DR GO; GO:0000403; F:Y-form DNA binding; IEA:Ensembl.
DR GO; GO:0008270; F:zinc ion binding; IEA:Ensembl.
DR GO; GO:0072757; P:cellular response to camptothecin; IEA:Ensembl.
DR GO; GO:0072711; P:cellular response to hydroxyurea; IEA:Ensembl.
DR GO; GO:0071479; P:cellular response to ionizing radiation; IEA:Ensembl.
DR GO; GO:0000729; P:DNA double-strand break processing; IEA:Ensembl.
DR GO; GO:0000724; P:double-strand break repair via homologous recombination; IEA:Ensembl.
DR GO; GO:0044806; P:G-quadruplex DNA unwinding; IEA:Ensembl.
DR GO; GO:0007095; P:mitotic G2 DNA damage checkpoint signaling; IEA:Ensembl.
DR GO; GO:0051782; P:negative regulation of cell division; IEA:Ensembl.
DR GO; GO:0045910; P:negative regulation of DNA recombination; IEA:Ensembl.
DR GO; GO:0045893; P:positive regulation of DNA-templated transcription; IEA:Ensembl.
DR GO; GO:0051260; P:protein homooligomerization; IEA:Ensembl.
DR GO; GO:0090329; P:regulation of DNA-templated DNA replication; IEA:Ensembl.
DR GO; GO:0031297; P:replication fork processing; IEA:Ensembl.
DR GO; GO:0071139; P:resolution of DNA recombination intermediates; IEA:Ensembl.
DR GO; GO:0010165; P:response to X-ray; IEA:Ensembl.
DR GO; GO:0061820; P:telomeric D-loop disassembly; IEA:Ensembl.
DR CDD; cd18016; DEXHc_RecQ2_BLM; 1.
DR CDD; cd18794; SF2_C_RecQ; 1.
DR Gene3D; 1.10.150.80; HRDC domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR012532; BDHCT.
DR InterPro; IPR032439; BDHCT_assoc.
DR InterPro; IPR032437; BLM_N.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR004589; DNA_helicase_ATP-dep_RecQ.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR010997; HRDC-like_sf.
DR InterPro; IPR002121; HRDC_dom.
DR InterPro; IPR044876; HRDC_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR032284; RecQ_Zn-bd.
DR InterPro; IPR018982; RQC_domain.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR NCBIfam; TIGR00614; recQ_fam; 1.
DR PANTHER; PTHR13710:SF105; BLOOM SYNDROME PROTEIN; 1.
DR PANTHER; PTHR13710; DNA HELICASE RECQ FAMILY MEMBER; 1.
DR Pfam; PF08072; BDHCT; 1.
DR Pfam; PF16204; BDHCT_assoc; 1.
DR Pfam; PF16202; BLM_N; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF00570; HRDC; 1.
DR Pfam; PF16124; RecQ_Zn_bind; 1.
DR Pfam; PF09382; RQC; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00341; HRDC; 1.
DR SMART; SM00956; RQC; 1.
DR SUPFAM; SSF47819; HRDC-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS50967; HRDC; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000005225}.
FT DOMAIN 666..841
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 867..1014
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT DOMAIN 1202..1282
FT /note="HRDC"
FT /evidence="ECO:0000259|PROSITE:PS50967"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 243..303
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1321..1401
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 243..283
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1326..1345
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1348..1372
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1401 AA; 157511 MW; 2E185E03D7A5395B CRC64;
MAGLPHNNLQ EQLERHSARK LNNKLSFSKP KSLGFTFKKK TPSDNSISLT SMSDNVTEAF
SVSEPPSHTT NQQTKINDFY KNAPAGQQTQ RVGSKPLLPD LVQIPKEVLC TTPHTSVIKK
PSDAAFKKLD FSSSSDSFGA IDNWDDMDDF DTSGTSKAFV TPLKNHFLLR VSTAQKSKKA
KRNFSKEQLY KTDTVKAGLT TSSSENKQVD FTKGQKDDSE RLSSDVICID DDCISEVRIN
EDTQESHSLK THLGDERDNS GKKKNLEDTE LHSTEKIPYT KLSDDYDTDF VPPSPEEESI
STASSSLKCF SMLQDSDNFD REKDVLSISK DTLSKPEKIT AQEPNPETST DYDARQISVQ
QQLIHVMDHI CKLIDTVPAD KLKALDCGDE LLLQRNVRRK LLAEVDFNRS GTSHLGSAWR
LRPDPCGSPV EEDSSGENFM KELAFPYLPS NPISPGEYLP TTALGKTGFL ATTKNHCESM
LLESSLQKSF VSNNWAETPK LEKSKESSFF PGSVLTSTAV KDQNKNTASI NDLEREIQAS
CEVDNFDIDD FDDDDDWENI MHNLAASKSS TTAHQPIREG RPVKSVSERI SSAKTNCLPV
ASTAQNINFS ETIQSYTDKS EQDSTYRNVK HEHFQSLNFP HTKEMLKIFH KKFGLHNFRT
NQLEAINAAL LGEDCFILMP TGGGKSLCYQ LPACVSPGVT VVISPLRSLI VDQVQKLTSL
DIPATYLTGD KTDSEATSIY LQLSKKDPII KLLYVTPEKV CASNRLISTL ENLYERKLLA
RFVIDEAHCV SQWGHDFRQD YKRMNMLRQK FPSVPVMALT ATANPRVQKD ILTQLKILRP
QVFSMSFNRH NLKYYVLPKK PKKVAFDCLE WIRKYHPYDS GIIYCLSRRE CDTMAGTLRK
DGLAALAYHA GLSDSERDEV QHKWINQDGC QVICATVAFG MGIDKPDVRF VIHASLPKSV
EGYYQESGRA GRDGEMSHCL LFYTYHDVTR LKRLIMMEKD GNHHTKETHF NNLYSMVHYC
ENITECRRIQ LLAYFGENGF NPDFCKKYPD VSCDNCCKRK DYKIRDVTDD VKSIIRFVQE
HSLSQRARNI KHIGSAGRFT MNMLVDIFLG SKSAKIQLGI FGKGSAYSRH NAERLFKKLI
LDKILDEDLY INANDQAIAY IMPGDKAQTV LSGHLKVDFM ETENSSSVKK QKALVAKVSQ
REEMVKKCLG ELTEVCKSLG KVFGVHYFNI FNIVTLKKLA ESLSSDPEVL LQIDGVTEDK
LEKYGAEVIA VLQKYSEWTF PGQYSKLGRA PAPYTKGGGF EPSPGQTALL LPGHQWPGWT
RPARKWRRPM SSKKVRQRRP HFRGSTACRK VSSKTKSSNV IGSSSASHDS QAPSGACRKL
GIMAPPKPVN RPFLKPSYAF S
//
