ID H0WSQ0_OTOGA Unreviewed; 1150 AA.
AC H0WSQ0;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=Alpha-mannosidase {ECO:0000256|RuleBase:RU361199};
DE EC=3.2.1.- {ECO:0000256|RuleBase:RU361199};
GN Name=MAN2A2 {ECO:0000313|Ensembl:ENSOGAP00000005119.2};
OS Otolemur garnettii (Small-eared galago) (Garnett's greater bushbaby).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Strepsirrhini; Lorisiformes;
OC Galagidae; Otolemur.
OX NCBI_TaxID=30611 {ECO:0000313|Ensembl:ENSOGAP00000005119.2, ECO:0000313|Proteomes:UP000005225};
RN [1] {ECO:0000313|Proteomes:UP000005225}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG The Broad Institute Genome Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K., Jaffe D.B.,
RA Gnerre S., MacCallum I., Przybylski D., Ribeiro F.J., Burton J.N.,
RA Walker B.J., Sharpe T., Hall G.;
RT "Version 3 of the genome sequence of Otolemur garnettii (Bushbaby).";
RL Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSOGAP00000005119.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|RuleBase:RU361199};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|RuleBase:RU361199};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 38 family.
CC {ECO:0000256|ARBA:ARBA00009792, ECO:0000256|RuleBase:RU361199}.
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DR EMBL; AAQR03183210; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03183211; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_003803126.1; XM_003803078.2.
DR AlphaFoldDB; H0WSQ0; -.
DR STRING; 30611.ENSOGAP00000005119; -.
DR Ensembl; ENSOGAT00000005729.2; ENSOGAP00000005119.2; ENSOGAG00000005723.2.
DR GeneID; 100957305; -.
DR KEGG; oga:100957305; -.
DR CTD; 4122; -.
DR eggNOG; KOG1958; Eukaryota.
DR GeneTree; ENSGT01030000234638; -.
DR HOGENOM; CLU_004690_1_0_1; -.
DR InParanoid; H0WSQ0; -.
DR OMA; GHQWLKY; -.
DR OrthoDB; 5474711at2759; -.
DR TreeFam; TF313152; -.
DR Proteomes; UP000005225; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004559; F:alpha-mannosidase activity; IEA:InterPro.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0016799; F:hydrolase activity, hydrolyzing N-glycosyl compounds; IEA:Ensembl.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006013; P:mannose metabolic process; IEA:InterPro.
DR Gene3D; 3.20.110.10; Glycoside hydrolase 38, N terminal domain; 1.
DR Gene3D; 1.20.1270.50; Glycoside hydrolase family 38, central domain; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR InterPro; IPR011682; Glyco_hydro_38_C.
DR InterPro; IPR015341; Glyco_hydro_38_cen.
DR InterPro; IPR037094; Glyco_hydro_38_cen_sf.
DR InterPro; IPR000602; Glyco_hydro_38_N.
DR InterPro; IPR027291; Glyco_hydro_38_N_sf.
DR InterPro; IPR028995; Glyco_hydro_57/38_cen_sf.
DR InterPro; IPR013780; Glyco_hydro_b.
DR PANTHER; PTHR11607; ALPHA-MANNOSIDASE; 1.
DR PANTHER; PTHR11607:SF57; ALPHA-MANNOSIDASE 2X; 1.
DR Pfam; PF09261; Alpha-mann_mid; 1.
DR Pfam; PF07748; Glyco_hydro_38C; 1.
DR Pfam; PF01074; Glyco_hydro_38N; 1.
DR SMART; SM00872; Alpha-mann_mid; 1.
DR SUPFAM; SSF88688; Families 57/38 glycoside transferase middle domain; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF88713; Glycoside hydrolase/deacetylase; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361199};
KW Hydrolase {ECO:0000256|RuleBase:RU361199};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU361199};
KW Reference proteome {ECO:0000313|Proteomes:UP000005225};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU361199}.
FT TRANSMEM 7..26
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 502..588
FT /note="Glycoside hydrolase family 38 central"
FT /evidence="ECO:0000259|SMART:SM00872"
SQ SEQUENCE 1150 AA; 130468 MW; 03439297B7CEE821 CRC64;
MKLKKQVTVC GAVIFCVAVF SLYLMLDRVQ HDPTRHQNGG NFPRSQISVL QNRIEQLEQL
LEENHEIISH IKHSVLELTA NAEGPPALLP YYTANGSWVV PPEPRPSFLS ISPQDCQFAL
GSRGQKPELQ MLAVSEELPF DNVEGGVWRQ GFDISYGPHD WDAEDLQVFV VPHSHNDPGW
IKTFDKYYTE QTQHILNSMV SKLQEDPRRR FLWAEVSFFA KWWDNISAQK RAAVRRLVGN
GQLEIATGGW VMPDEANSHY FALIDQLIEG HQWLERNLGT TPRSAWAVDP FGYSSTMPYL
LRRANLTSML IQRVHYAIKK HFAATHSLEF MWRQTWDPDS STDIFCHMMP FYSYDIPHTC
GPDPKICCQF DFKRLPGGRI NCPWKVPPRA ITEANIAERA ALLLDQYRKK SRLFRSNVLL
VPLGDDFRYD KPQEWDAQFF NYQRLFDFFS SRPDLHVQAQ FGTLSDYFDA LYKRTGVEPG
AQPPGFPVLS GDFFSYADRE DHYWTGYYTS RPFYKSLDRV LEAHLRGAEI LYSLAVAHAR
RSGLAGQYPL SDFTLLTEAR RTLGLFQHHD AITGTAKEAV VVDYGVRLLR SLVSLKRVII
NAAHYLVLGD RGAYHFDPEV PFLQMDDTRL SHDALPERTV IQLDSSPRFV VLFNPLEHER
LSVVSLLVNS PRVRVLSEDG QPLAVQIGMY WSSATEVVPD VFQVSVPIRL PALGLGVLQL
HLGLDGHRTL PSSVRVYLHG RQLSISRHEA FPLRIIESAS SDFTLSNRYM QFWFSGLTGL
LKSIRRVDEE PEQQVDMEFL VYGTRASKDK SGAYLFLPDG EAKPYVPKEP PVLRVTEGPF
FSEVAVYYEH VHQMVRLYNV PGVEGLSLDV SLLVDIRDYV NKELALRVHT DIESQGTFFT
DLNGFQVQPR RYLKKLPLQA NFYPMPVMAY IQDAQKRLTL HTAQALGVSS LRDGQLEVIL
DRRLMQDDNR GLGQGLKDNK RTHSSFRLLL ERRAIGTEVQ DSHPTSYPSL LSHLTSAHLN
TPVLALPVAQ RPLPGPGLRS FHPLASSLPC DFHLLNLRAL QAEDDTLPSA ETALLLHRKG
FDCGLEAKNL GFNCTTSQGK VALGSLFHGL DVVFLQPTSL TLLYPLASPS NSTDVYLEPM
EIATFRLRLG
//
