ID H0WVG4_OTOGA Unreviewed; 673 AA.
AC H0WVG4;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 58.
DE RecName: Full=Thyrotropin releasing hormone degrading enzyme {ECO:0008006|Google:ProtNLM};
OS Otolemur garnettii (Small-eared galago) (Garnett's greater bushbaby).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Strepsirrhini; Lorisiformes;
OC Galagidae; Otolemur.
OX NCBI_TaxID=30611 {ECO:0000313|Ensembl:ENSOGAP00000006299.2, ECO:0000313|Proteomes:UP000005225};
RN [1] {ECO:0000313|Proteomes:UP000005225}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG The Broad Institute Genome Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K., Jaffe D.B.,
RA Gnerre S., MacCallum I., Przybylski D., Ribeiro F.J., Burton J.N.,
RA Walker B.J., Sharpe T., Hall G.;
RT "Version 3 of the genome sequence of Otolemur garnettii (Bushbaby).";
RL Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSOGAP00000006299.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR634016-3};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|PIRSR:PIRSR634016-3};
CC -!- SIMILARITY: Belongs to the peptidase M1 family.
CC {ECO:0000256|ARBA:ARBA00010136}.
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DR EMBL; AAQR03149324; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03149325; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03149326; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03149327; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03149328; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03149329; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03149330; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03149331; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03149332; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03149333; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; H0WVG4; -.
DR STRING; 30611.ENSOGAP00000006299; -.
DR Ensembl; ENSOGAT00000007041.2; ENSOGAP00000006299.2; ENSOGAG00000007037.2.
DR eggNOG; KOG1046; Eukaryota.
DR GeneTree; ENSGT00940000155878; -.
DR HOGENOM; CLU_003705_5_2_1; -.
DR InParanoid; H0WVG4; -.
DR OMA; CHPHTSN; -.
DR TreeFam; TF300395; -.
DR Proteomes; UP000005225; Unassembled WGS sequence.
DR GO; GO:0004177; F:aminopeptidase activity; IEA:UniProt.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09601; M1_APN-Q_like; 1.
DR Gene3D; 1.25.50.20; -; 1.
DR Gene3D; 2.60.40.1910; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR InterPro; IPR024571; ERAP1-like_C_dom.
DR InterPro; IPR034016; M1_APN-typ.
DR InterPro; IPR001930; Peptidase_M1.
DR InterPro; IPR014782; Peptidase_M1_dom.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR PANTHER; PTHR11533; PROTEASE M1 ZINC METALLOPROTEASE; 1.
DR PANTHER; PTHR11533:SF40; THYROTROPIN-RELEASING HORMONE-DEGRADING ECTOENZYME; 1.
DR Pfam; PF11838; ERAP1_C; 1.
DR Pfam; PF01433; Peptidase_M1; 1.
DR PRINTS; PR00756; ALADIPTASE.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR634016-3};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000005225};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|PIRSR:PIRSR634016-3}.
FT DOMAIN 17..240
FT /note="Peptidase M1 membrane alanine aminopeptidase"
FT /evidence="ECO:0000259|Pfam:PF01433"
FT DOMAIN 367..650
FT /note="ERAP1-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF11838"
FT ACT_SITE 90
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-1"
FT BINDING 89
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 93
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT BINDING 112
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-3"
FT SITE 176
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR634016-4"
SQ SEQUENCE 673 AA; 78008 MW; 07C444CDFCF212C8 CRC64;
VRLYARPDAI RRGSGDYALH ITKRLIEFYE DYFKVPYSLP KLDLLAVPKH PYAAMENWGL
SIFVEQRILL DPSVSSISYL LDVTMVIVHE ICHQWFGDLV TPVWWEDVWL KEGFAHYFEF
VGTDYLYPGW NMEKQRFLTD VLHEVMLLDG LASSHPVSQE VLQATDIDRV FDWIAYKKGA
ALIRMLANFM GHSVFQRGLQ DYLTIHKYGN AARNDLWNTL SEALKRNGKY VNIQAVMDQW
TLQMGYPVIT ILGNTTTTNK VIITQQHFIY DIGAKTKALE LRNSSYLWHI PLTIVVGNRS
HVSSEAIIWV SNKSVNYHLL LLNIHTYIQE NVPTARSNKV TKNINSYNHR FNTSLRQSET
LSLKIARHYI SDAFLEAVAG YLPQNIPLEI LRYLSAEKDF LPWHAASRAL YPLDKLLDRM
ENYNVFNEYI LKQVATTYIK LGWPKNNFNG SLVQASYQHE ELRREVIMLA CSFGNKHCHQ
QASTLISDWI SSNRNRIPLN VRDIVYCTGV SLLDEDVWEF IWMKFHSTTA VSEKKILLEA
LTCSDDRNLL NRLLNLSLNS EVVLDQDAID VIIHVARNPH GRDLAWKFFR DKWKILNTRY
GEALFMNSKL ISGVTEFLNT EGELKELRSF MKSYDGVAAA SFSRAVETVE ANVRWKMLYQ
DELFQWLGKA LRH
//
