ID H0WYT9_OTOGA Unreviewed; 1202 AA.
AC H0WYT9;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 61.
DE SubName: Full=Solute carrier family 12 member 2 {ECO:0000313|Ensembl:ENSOGAP00000007706.2};
GN Name=SLC12A2 {ECO:0000313|Ensembl:ENSOGAP00000007706.2};
OS Otolemur garnettii (Small-eared galago) (Garnett's greater bushbaby).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Strepsirrhini; Lorisiformes;
OC Galagidae; Otolemur.
OX NCBI_TaxID=30611 {ECO:0000313|Ensembl:ENSOGAP00000007706.2, ECO:0000313|Proteomes:UP000005225};
RN [1] {ECO:0000313|Proteomes:UP000005225}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG The Broad Institute Genome Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K., Jaffe D.B.,
RA Gnerre S., MacCallum I., Przybylski D., Ribeiro F.J., Burton J.N.,
RA Walker B.J., Sharpe T., Hall G.;
RT "Version 3 of the genome sequence of Otolemur garnettii (Bushbaby).";
RL Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSOGAP00000007706.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 chloride(out) + K(+)(out) + Na(+)(out) = 2 chloride(in) +
CC K(+)(in) + Na(+)(in); Xref=Rhea:RHEA:72395, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:72396;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC {ECO:0000256|ARBA:ARBA00010593}.
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DR EMBL; AAQR03010183; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03010184; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03010185; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03010186; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03010187; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03010188; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03010189; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03010190; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03010191; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03010192; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR STRING; 30611.ENSOGAP00000007706; -.
DR Ensembl; ENSOGAT00000008604.2; ENSOGAP00000007706.2; ENSOGAG00000008598.2.
DR eggNOG; KOG2083; Eukaryota.
DR GeneTree; ENSGT00940000155742; -.
DR HOGENOM; CLU_001883_0_0_1; -.
DR InParanoid; H0WYT9; -.
DR OMA; DTISFEM; -.
DR TreeFam; TF313191; -.
DR Proteomes; UP000005225; Unassembled WGS sequence.
DR GO; GO:0016324; C:apical plasma membrane; IEA:Ensembl.
DR GO; GO:0009925; C:basal plasma membrane; IEA:Ensembl.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:Ensembl.
DR GO; GO:0016328; C:lateral plasma membrane; IEA:Ensembl.
DR GO; GO:0043005; C:neuron projection; IEA:Ensembl.
DR GO; GO:0043025; C:neuronal cell body; IEA:Ensembl.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IEA:Ensembl.
DR GO; GO:0051879; F:Hsp90 protein binding; IEA:Ensembl.
DR GO; GO:0019901; F:protein kinase binding; IEA:Ensembl.
DR GO; GO:0051087; F:protein-folding chaperone binding; IEA:Ensembl.
DR GO; GO:0008511; F:sodium:potassium:chloride symporter activity; IEA:Ensembl.
DR GO; GO:1990869; P:cellular response to chemokine; IEA:Ensembl.
DR GO; GO:0098659; P:inorganic cation import across plasma membrane; IEA:Ensembl.
DR GO; GO:0010818; P:T cell chemotaxis; IEA:Ensembl.
DR GO; GO:0070634; P:transepithelial ammonium transport; IEA:Ensembl.
DR GO; GO:0030321; P:transepithelial chloride transport; IEA:Ensembl.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR013612; AA_permease_N.
DR InterPro; IPR002444; NKCC1.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR002443; SLC12A1/SLC12A2.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF58; SOLUTE CARRIER FAMILY 12 MEMBER 2; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 1.
DR Pfam; PF08403; AA_permease_N; 1.
DR Pfam; PF03522; SLC12; 1.
DR PRINTS; PR01207; NAKCLTRNSPRT.
DR PRINTS; PR01208; NAKCLTRSPRT1.
PE 3: Inferred from homology;
KW Ion transport {ECO:0000256|ARBA:ARBA00023201};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000005225};
KW Sodium {ECO:0000256|ARBA:ARBA00023053};
KW Sodium transport {ECO:0000256|ARBA:ARBA00023201};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00023201}.
FT TRANSMEM 280..300
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 306..332
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 353..377
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 397..416
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 425..446
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 476..498
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 510..530
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 588..611
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 644..664
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 670..688
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 700..719
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 725..740
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 191..242
FT /note="Amino acid permease N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08403"
FT DOMAIN 279..781
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 790..1202
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT REGION 1..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 139..186
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 949..986
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..180
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 949..978
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1202 AA; 130607 MW; 32F957AC129F0742 CRC64;
MEPGPAAPSS GAQRSAGVGE TPPAAALAPA GLDLPGTAVP SVPEDAAAAS RDASGVRSEV
AAAAGDGLGR PLGPTPSQSR FQVDLVSENA GRAAAAAAAA GAEKAAKEAK VSGEGGPAKG
SEEAKGRFRV NFVDPAASSS AEDSLSDAAG IGGDGPNVSF QNGGDTVLSE GSSLHSGGGG
SSGHHQHYYY DTHTNTYYLR TFGHNTMDAV PRIDHYRHTA AQLGEKLLRP SLAELHDELE
KEPFEDGFAN GEESTPTRDA VVTYAAESKG VVKFGWIKGV LVRCMLNIWG VMLFIRLSWI
VGQAGIGLSV VVIMMATVVT TITGLSTSAI ATNGFVRGGG AYYLISRSLG PEFGGAIGLI
FAFANAVAVA MYVVGFAETV VELLKEHSIL MIDEINDIRI IGAITVVILL GISVAGMEWE
AKAQIVLLVI LLFAIGDFVI GTFIPLESKK PKGFFGYKSE IFNENFGPDF RQDETFFSVF
AIFFPAATGI LAGANISGDL ADPQSAIPKG TLLAILITTL VYVGIAVSVG SCVVRDATGN
VNDTIVTELT NCTSAACKLN FDFSFCESNP CSYGLMNNFQ VMSMVSGFAP LISAGIFSAT
LSSALASLVS APKIFQALCK DNIYPAFQMF AKGYGKNNEP LRGYILTFLI ALGFILIAEL
NVIAPIISNF FLASYALINF SVFHASLAKS PGWRPAFKYY NMWISLIGAI LCCIVMFVIN
WWAALLTYVI VLGLYIYVTY KKPDVNWGSS TQALTYLNAL QHSIRLSGVE DHVKNFRPQC
LVMTGAPNSR PALLHLVHDF TKNVGLMICG HVHMGPRRQA MKEMSIDQTK YQRWLIKNKM
KAFYAAVHAD DLREGAQYLM QVSLLCHSNA CVLVLGIKKL YLQMDVKSSD YFLNHFVDAF
DIQYGVVVIR LKEGLDISHL QGQEELLSSQ EKSPGPKDVV LSVEYSKKSD LDTSKPPGEK
PVKDNLDEEE DGKTPTQPLL KKEKXGPAVP LNVADQKLLE ASTQFQKKQG KNTIDVWWLF
DDGGLTLLIP YLLTTKKKWK DCKIRVFIGG KINRIDHDRR AMATLLSKFR IDFSDIMVLG
DINTKPKKEN ITAFDEMIEP YRLHEDDKEQ DIADKMKEDE PWRITDNELE LYKTKTYRQI
RLNELLKEHS STANIIVMSL PVARKGAVSS ALYMAWLEAL SKDLPPILLV RGNHQSVLTF
YS
//
