ID H0XAR0_OTOGA Unreviewed; 1353 AA.
AC H0XAR0;
DT 22-FEB-2012, integrated into UniProtKB/TrEMBL.
DT 22-FEB-2012, sequence version 1.
DT 27-MAR-2024, entry version 66.
DE RecName: Full=DNA polymerase {ECO:0000256|RuleBase:RU000442};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU000442};
GN Name=POLA1 {ECO:0000313|Ensembl:ENSOGAP00000012725.2};
OS Otolemur garnettii (Small-eared galago) (Garnett's greater bushbaby).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Strepsirrhini; Lorisiformes;
OC Galagidae; Otolemur.
OX NCBI_TaxID=30611 {ECO:0000313|Ensembl:ENSOGAP00000012725.2, ECO:0000313|Proteomes:UP000005225};
RN [1] {ECO:0000313|Proteomes:UP000005225}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG The Broad Institute Genome Sequencing Platform;
RA Di Palma F., Johnson J., Lander E.S., Lindblad-Toh K., Jaffe D.B.,
RA Gnerre S., MacCallum I., Przybylski D., Ribeiro F.J., Burton J.N.,
RA Walker B.J., Sharpe T., Hall G.;
RT "Version 3 of the genome sequence of Otolemur garnettii (Bushbaby).";
RL Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSOGAP00000012725.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|RuleBase:RU000442};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC {ECO:0000256|ARBA:ARBA00005755, ECO:0000256|RuleBase:RU000442}.
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DR EMBL; AAQR03085760; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03085761; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03085762; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03085763; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03085764; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03085765; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03085766; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AAQR03085767; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR STRING; 30611.ENSOGAP00000012725; -.
DR Ensembl; ENSOGAT00000014205.2; ENSOGAP00000012725.2; ENSOGAG00000014195.2.
DR eggNOG; KOG0970; Eukaryota.
DR GeneTree; ENSGT00550000074891; -.
DR HOGENOM; CLU_001718_0_0_1; -.
DR InParanoid; H0XAR0; -.
DR OMA; MTKMNVG; -.
DR TreeFam; TF103001; -.
DR Proteomes; UP000005225; Unassembled WGS sequence.
DR GO; GO:0005658; C:alpha DNA polymerase:primase complex; IEA:Ensembl.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProt.
DR GO; GO:0006269; P:DNA replication, synthesis of RNA primer; IEA:Ensembl.
DR GO; GO:1902975; P:mitotic DNA replication initiation; IEA:InterPro.
DR CDD; cd05776; DNA_polB_alpha_exo; 1.
DR CDD; cd05532; POLBc_alpha; 1.
DR Gene3D; 2.40.50.730; -; 1.
DR Gene3D; 3.30.70.2820; -; 1.
DR Gene3D; 1.10.3200.20; DNA Polymerase alpha, zinc finger; 1.
DR Gene3D; 1.10.132.60; DNA polymerase family B, C-terminal domain; 1.
DR Gene3D; 1.10.287.690; Helix hairpin bin; 1.
DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR024647; DNA_pol_a_cat_su_N.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR038256; Pol_alpha_znc_sf.
DR InterPro; IPR045846; POLBc_alpha.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR015088; Znf_DNA-dir_DNA_pol_B_alpha.
DR NCBIfam; TIGR00592; pol2; 1.
DR PANTHER; PTHR45861; DNA POLYMERASE ALPHA CATALYTIC SUBUNIT; 1.
DR PANTHER; PTHR45861:SF1; DNA POLYMERASE ALPHA CATALYTIC SUBUNIT; 1.
DR Pfam; PF12254; DNA_pol_alpha_N; 1.
DR Pfam; PF00136; DNA_pol_B; 1.
DR Pfam; PF03104; DNA_pol_B_exo1; 1.
DR Pfam; PF08996; zf-DNA_Pol; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|RuleBase:RU000442};
KW DNA-binding {ECO:0000256|RuleBase:RU000442};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU000442};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU000442};
KW Reference proteome {ECO:0000313|Proteomes:UP000005225};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000442};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 41..102
FT /note="DNA polymerase alpha catalytic subunit N-terminal"
FT /evidence="ECO:0000259|Pfam:PF12254"
FT DOMAIN 416..716
FT /note="DNA-directed DNA polymerase family B exonuclease"
FT /evidence="ECO:0000259|Pfam:PF03104"
FT DOMAIN 781..1235
FT /note="DNA-directed DNA polymerase family B
FT multifunctional"
FT /evidence="ECO:0000259|Pfam:PF00136"
FT DOMAIN 1276..1353
FT /note="Zinc finger DNA-directed DNA polymerase family B
FT alpha"
FT /evidence="ECO:0000259|Pfam:PF08996"
FT REGION 1..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 110..134
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 190..231
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 190..220
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1353 AA; 153260 MW; B10DF82C64551B85 CRC64;
MAPVHGDDCE LGASAVSDSG SFVASRARRE KKSKKGRQEA LERLKKAKAG EKFKYEVEDF
ASVYEEVDEE QYSKLVQARQ DDDWIVDDDG IGYVEDGREI FDDDLEDDAF DVSEKGKNGR
ARNKDKRNVK KPKVTKPNNI KSMFIACAGK KTADKGVDLS KDDLLGNILQ DLNTETPQIT
PPPVMVLQKK RSTGTSLNPF SVHTPKAVPS GKTGSSISRK EPPLTPVPLK CPEFSGEMVQ
EEHKEDKQDS GAMEFEDGDF DEPMEAEEVD VEPVAAKTWD QKSEPAERVK HEEGLGKGTT
SCLGSFLPDV SCWDIDQEDD NSFSAQEVQV DSSYLPLVKG ADEEQVFQFY WLDAYEDQYN
QPGVVFLFGK VWIESAKTHV SCCVMVKNIE RTLYFLPRET KIDRNTGKET GTPVAIKDVY
DEFDEKIAAK YKIMKFKSKI VEKNYAFEIP DVPEKSEYLE VRYSAEMPQL PQDLKGETFS
HVFGTNTSSL ELFLMSRKIK GPCWLEVKNP QLLNQPISWC KVEAMALKPD LVNVIKDVSP
PPLVVMSFSM KTMQDAKDHQ NVIIAIAALV HHNFALDKAP PQPPFQSHFC VVSKPMDCIF
PYAFKEVIEK KNMKIEVAAT ERTLLGFFLA KVHKIDPDII VGHNIYGFEL EVLLQRINVG
KVPYWSKIGR LKRCNMPKLG GRSGFGERNA TCGRIICDVE ISAKELIRCK SYHLSELVQQ
ILKTERVVIP MENIRNMYSE SSHLLYLLEH TWKDARFILQ IMCELNVLPL ALQITNIAGN
IMSRTLMGGR SERNEFLLLH AFYENNYIVP DKQIFRKPPQ KLGDEDEEID GDTKYKKGRK
KAAYAGGLVL DPKVGFYDKF ILLLDFNSLY PSIIQEFNIC FTTVQRVALE AKKATEDVEQ
EQIPELPDPG LEMGILPREI RKLVERRKVI SCKPIGRGQG LLLSLCGLQY DIRQKALKLT
ANSMYGCLGF SFSRFYAKPL AALVTYKGRE ILMHTKEMVQ KMNLEVIYGD TDSIMINTNS
TNLEEVFKLG NKVKNEVNKL YKLLEIDIDG VFKSLLLLKK KKYAALVVEP TSDGNYFTKQ
ELKGLDIIRR DWCDLAKDTG NFVIGQILSD QSRDTIVESI QKRLMEIGEN VLNGCVPVSQ
FEINKALTKD PQDYPDKKSL PHVHVALWIN SQGGRKMKAG DTVSYVICQD GSNLSANQRA
YAPEQLQKQD SLTIDTQYYL AQQIHPVVAR ICEPIDGIDS VLIATWLVIG LDPTQFKVHD
YHKDEENDAL LGGPAQLTDE EKYRDCERFK CPCPTCGTEN IYDNVFDGLG TEMEPSLYRC
SNIECKASPL TFIVQLSNKL IMDIRRFIKK YYD
//
