ID H2L566_ORYLA Unreviewed; 1112 AA.
AC H2L566;
DT 21-MAR-2012, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 2.
DT 27-MAR-2024, entry version 83.
DE RecName: Full=Ras protein-specific guanine nucleotide-releasing factor 2a {ECO:0008006|Google:ProtNLM};
GN Name=rasgrf2a {ECO:0000313|Ensembl:ENSORLP00000000932.2};
OS Oryzias latipes (Japanese rice fish) (Japanese killifish).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Beloniformes; Adrianichthyidae; Oryziinae;
OC Oryzias.
OX NCBI_TaxID=8090 {ECO:0000313|Ensembl:ENSORLP00000000932.2, ECO:0000313|Proteomes:UP000001038};
RN [1] {ECO:0000313|Ensembl:ENSORLP00000000932.2, ECO:0000313|Proteomes:UP000001038}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hd-rR {ECO:0000313|Ensembl:ENSORLP00000000932.2,
RC ECO:0000313|Proteomes:UP000001038};
RX PubMed=17554307; DOI=10.1038/nature05846;
RA Kasahara M., Naruse K., Sasaki S., Nakatani Y., Qu W., Ahsan B., Yamada T.,
RA Nagayasu Y., Doi K., Kasai Y., Jindo T., Kobayashi D., Shimada A.,
RA Toyoda A., Kuroki Y., Fujiyama A., Sasaki T., Shimizu A., Asakawa S.,
RA Shimizu N., Hashimoto S., Yang J., Lee Y., Matsushima K., Sugano S.,
RA Sakaizumi M., Narita T., Ohishi K., Haga S., Ohta F., Nomoto H., Nogata K.,
RA Morishita T., Endo T., Shin-I T., Takeda H., Morishita S., Kohara Y.;
RT "The medaka draft genome and insights into vertebrate genome evolution.";
RL Nature 447:714-719(2007).
RN [2] {ECO:0000313|Ensembl:ENSORLP00000000932.2}
RP IDENTIFICATION.
RC STRAIN=Hd-rR {ECO:0000313|Ensembl:ENSORLP00000000932.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
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DR AlphaFoldDB; H2L566; -.
DR STRING; 8090.ENSORLP00000000932; -.
DR Ensembl; ENSORLT00000000933.2; ENSORLP00000000932.2; ENSORLG00000000753.2.
DR GeneTree; ENSGT00940000155679; -.
DR HOGENOM; CLU_003405_0_1_1; -.
DR InParanoid; H2L566; -.
DR Proteomes; UP000001038; Chromosome 12.
DR Bgee; ENSORLG00000000753; Expressed in testis and 10 other cell types or tissues.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IBA:GO_Central.
DR GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR CDD; cd06224; REM; 1.
DR CDD; cd00160; RhoGEF; 1.
DR Gene3D; 1.20.900.10; Dbl homology (DH) domain; 1.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 2.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR Gene3D; 1.20.870.10; Son of sevenless (SoS) protein Chain: S domain 1; 2.
DR InterPro; IPR035899; DBL_dom_sf.
DR InterPro; IPR000219; DH-domain.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR008937; Ras-like_GEF.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR PANTHER; PTHR23113; GUANINE NUCLEOTIDE EXCHANGE FACTOR; 1.
DR PANTHER; PTHR23113:SF359; RAS-SPECIFIC GUANINE NUCLEOTIDE-RELEASING FACTOR 2-LIKE ISOFORM X1; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR Pfam; PF00621; RhoGEF; 1.
DR SMART; SM00233; PH; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 2.
DR SMART; SM00325; RhoGEF; 1.
DR SUPFAM; SSF48065; DBL homology domain (DH-domain); 1.
DR SUPFAM; SSF50729; PH domain-like; 2.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR PROSITE; PS50010; DH_2; 1.
DR PROSITE; PS50096; IQ; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658,
KW ECO:0000256|PROSITE-ProRule:PRU00168};
KW Reference proteome {ECO:0000313|Proteomes:UP000001038}.
FT DOMAIN 22..117
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 227..413
FT /note="DH"
FT /evidence="ECO:0000259|PROSITE:PS50010"
FT DOMAIN 608..726
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50212"
FT DOMAIN 969..1112
FT /note="Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50009"
FT REGION 736..772
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 803..841
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 135..172
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 736..758
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1112 AA; 126277 MW; CB98157146D9AFD5 CRC64;
MQKSVRYNEG HALYLSLIAR KEGTKRGFLS KKTTENSRWT EKYFALHQNL LFYFENEQST
RPSGIYLLEG CTCERTPAPK HYFLVIFGHD GQKPLELRTD EESDCNEWVE CIQQASYSDI
IIEREILMQK YIHLVQILET EKIAANQLRT QLEDQDTEIE RLKAEIVVLN KTKERMLPYQ
NNQEEEDPDI KKIKKVQSFM RGWICRRKWK IIVQDYICSP HAESMRKRNQ IVFTMVEAEA
EYVHQLSILV NCFLRPLRMA ASSKKPPISH DDVSSIFLNS ETIMFLHEIF HQGLKARIAS
WPTLVLADLF DILLPMLNIY QEFVRNHQYS LQVLANCKQN RDFDKLLKQY ESNAACEGRM
LETFLTYPMF QIPRYIITLH ELLAHTPHEH VERKSLEFAK SKLEELSKMM HDEVSDTENI
RKNLAIERMI VEGCDILLDT SQTFVRQGSL IQLPSSSERG VLSKVRLGSL SLRKEGERQC
FLFTKHFLIC TRTSGGKLHL LKQGGTLSLI ECTLIEELDA SDEDCECLCT FLPYAGTRDR
LTWSRPNVSG SCVSLPSQCI DNIRCNGLMT SVFEENSKVS VPHMIKSDAR LHKDDVDICF
SKTLNSCKVP QIRYASVERL LERLTDLRFL SIDFLNTFLH TYRIFTTGAV VIDKLADIYK
KPFTSIPVRS LEVFFATNQG AWGADPLNNK SPRLCRKFSS PPPLSIPSRT SSPVHCRKLS
LSSPISIKVG ALDLSTSPSS SAANSPTSSH TPLDLSRGPS SPELSPAAAE DVSGELPRLD
AFCGKLRRSI RRAVLESVSL DKFIPESPSS GEPGDLSPCR SPSTPRHLRF RQSGAAAAEG
SRMSPASAFA IATAAAGHSS SQGFMNSEKG CDKEFIIRRA ATNRVLNVLR HWVSKHSQDF
DMNSELKTGV MSLLEEVLRD PDLLPQERKA TTNILSALSQ EDQDDSQLRI EDILQMAESP
KVECIESLSA MELAEQITLL DHIVFRSIPY EEFLGQGWMK IDKSERTPFI MKTSQHFNDM
SNLVASHIIS HTDVGSRASS IDKWLAVADI CRCLNNYNGV LEITSALNRS AVYRLKKTWA
KVCKQTKALM DRLQKIVSSE GRFKNLRETL KK
//
