ID H2L671_ORYLA Unreviewed; 1707 AA.
AC H2L671;
DT 21-MAR-2012, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 2.
DT 27-MAR-2024, entry version 73.
DE RecName: Full=Rap guanine nucleotide exchange factor (GEF) 6 {ECO:0008006|Google:ProtNLM};
GN Name=rapgef6 {ECO:0000313|Ensembl:ENSORLP00000001315.2};
OS Oryzias latipes (Japanese rice fish) (Japanese killifish).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Beloniformes; Adrianichthyidae; Oryziinae;
OC Oryzias.
OX NCBI_TaxID=8090 {ECO:0000313|Ensembl:ENSORLP00000001315.2, ECO:0000313|Proteomes:UP000001038};
RN [1] {ECO:0000313|Ensembl:ENSORLP00000001315.2, ECO:0000313|Proteomes:UP000001038}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hd-rR {ECO:0000313|Ensembl:ENSORLP00000001315.2,
RC ECO:0000313|Proteomes:UP000001038};
RX PubMed=17554307; DOI=10.1038/nature05846;
RA Kasahara M., Naruse K., Sasaki S., Nakatani Y., Qu W., Ahsan B., Yamada T.,
RA Nagayasu Y., Doi K., Kasai Y., Jindo T., Kobayashi D., Shimada A.,
RA Toyoda A., Kuroki Y., Fujiyama A., Sasaki T., Shimizu A., Asakawa S.,
RA Shimizu N., Hashimoto S., Yang J., Lee Y., Matsushima K., Sugano S.,
RA Sakaizumi M., Narita T., Ohishi K., Haga S., Ohta F., Nomoto H., Nogata K.,
RA Morishita T., Endo T., Shin-I T., Takeda H., Morishita S., Kohara Y.;
RT "The medaka draft genome and insights into vertebrate genome evolution.";
RL Nature 447:714-719(2007).
RN [2] {ECO:0000313|Ensembl:ENSORLP00000001315.2}
RP IDENTIFICATION.
RC STRAIN=Hd-rR {ECO:0000313|Ensembl:ENSORLP00000001315.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004236}.
CC -!- SIMILARITY: Belongs to the RAPGEF2 family.
CC {ECO:0000256|ARBA:ARBA00010829}.
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DR RefSeq; XP_011481476.1; XM_011483174.1.
DR Ensembl; ENSORLT00000001316.2; ENSORLP00000001315.2; ENSORLG00000001061.2.
DR GeneID; 101165993; -.
DR CTD; 51735; -.
DR GeneTree; ENSGT00940000158124; -.
DR HOGENOM; CLU_002782_2_0_1; -.
DR OrthoDB; 5473909at2759; -.
DR Proteomes; UP000001038; Chromosome 14.
DR Bgee; ENSORLG00000001061; Expressed in animal zygote and 14 other cell types or tissues.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-SubCell.
DR GO; GO:0005770; C:late endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR CDD; cd00038; CAP_ED; 2.
DR CDD; cd00992; PDZ_signaling; 1.
DR CDD; cd01785; RA_PDZ-GEF1; 1.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 2.30.42.10; -; 1.
DR Gene3D; 2.60.120.10; Jelly Rolls; 2.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR Gene3D; 1.20.870.10; Son of sevenless (SoS) protein Chain: S domain 1; 1.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR018490; cNMP-bd_dom_sf.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR45161; CYTOSKELETON-ASSOCIATED PROTEIN 4; 1.
DR PANTHER; PTHR45161:SF4; RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 6; 1.
DR Pfam; PF00595; PDZ; 1.
DR Pfam; PF00788; RA; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00100; cNMP; 1.
DR SMART; SM00228; PDZ; 1.
DR SMART; SM00314; RA; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF51206; cAMP-binding domain-like; 2.
DR SUPFAM; SSF50156; PDZ domain-like; 1.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS50106; PDZ; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658,
KW ECO:0000256|PROSITE-ProRule:PRU00168};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Neurogenesis {ECO:0000256|ARBA:ARBA00022902};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000001038}.
FT DOMAIN 316..416
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 448..561
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50212"
FT DOMAIN 566..636
FT /note="PDZ"
FT /evidence="ECO:0000259|PROSITE:PS50106"
FT DOMAIN 791..877
FT /note="Ras-associating"
FT /evidence="ECO:0000259|PROSITE:PS50200"
FT DOMAIN 902..1130
FT /note="Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50009"
FT REGION 173..278
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1181..1314
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1428..1459
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1496..1577
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1655..1707
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 218..245
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 261..277
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1181..1223
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1235..1250
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1264..1314
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1522..1544
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1561..1577
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1707 AA; 189693 MW; F8AF768698A0656C CRC64;
MLSLERPFPQ GCEFLRTHGD SDTYGDDGEA TEVMKMTGAM EASFKLALKK PPSVRTAEDL
QTIYCHLYNM EVLSHLREHQ LRSMCSSAKY ERHEANHILF DPDSIATCWY ILLSGSVFIK
EHMYLARCCF GKQLGGRRGC ECITLEPSEM IVVDNGSEGN DCFLQREGSQ RQSRRRFRRV
NPRGERELIT DGQEPTGYNT LPADLSKMHL TDHAHQQAMH MPPSQSGCSI ASDSGSSSLS
DIYQATESEL GDVDLSGLPE AAVDSEEEEE EDEDLERASD ALLGRDVVRE CLEKDPADRH
DDDIEQLLEF MHQLPAFANM TMSVRRELCK VMVFEVVEQA GHVILRDKQE LDNWRVILNG
AVEISYPDGR EETLCMGNSF GISPSLEKQY MCGEVRTKGD DCQFVCIAKE DYWRILNHVE
KNTHKVEEEG EIVMVKEHRE LDRSGTRKGH IVIKGTPERL IMHLVEEPSV VDPTYIEDFL
LTYRTFLSSP MEVGKKLLEW FQKDSLQDTV TRIVLLWVNN HFNDFEGDPA MTRFLEEFEK
HLEATKMKGH LRLLNIACAA KAKLRQITLQ KASRESPLHF KLLGGSERGF GIFVEMVEEG
SKAAETGLKR GDQIMEINGQ SFENISFSKA LDILKNNTHL SLTVKTNIFV FKELHSRILN
EKKNGGPHIP KIQEKKGNRL SIPELPGDME FATDHKATRK MKANTVSGGR NRIRKMLEKT
RFSILPPKPF NDGGIGQSQD DSMVGTKQCR HSVVMMPIPS NLSSSSPDLL QPASNVLDFS
NPAALGLYYI PDQVIRVFKA DQQSCYIIIS KDTTAKDVVA HAVNEFGLSA APESYSLCEV
SVSPEGVTKQ RRLPDQLSKL ADRIQLNGRY YLKNNMDTET LCSDDDAQDL LKESQISLLQ
LSTMEVAAQL SMRDFELFRN IEPTEYVDDL FKLDSPLSRG NLKRFEEVMN QETFWVATEI
LREPNSVKRM RTIKHFIKIA LHCKECKNFN SMFAIISGLD LPPVARLRSS WEKLPSKYAK
LFGDLQDVFD PSRNMAKYRN MLSSQSMQPP IIPLFPVIKK DLTFFHEGND SSVDGLVNFE
KLRMIAKEIR HVVRMTSANM DPALMFRQRS LSQGSTNSNM LDVQGGAHKK RVRRSSLLNA
KKLYEDAQMA RKVKQYLSNL TVETDEEKHQ IMSLQCEPSY NTLSKNMNER RPTKSNMSPV
SLRSALQSGK TQNRVSQVLQ VQVPLNPLRK KSLAKDNGTP TSSSPQVIKK PPVNSDDWVT
KRPSEDTISN ISSLHSSPSL SYQNSPLKAG LAKSHNSSQM NLSGSSSSLT SDASTKTGTV
SLRSFGIGYS LLPPGKADNL SDSSHSEISS RSSICSVDSV PAHGSDERCG RTCTASSTMA
ASTTTGAAVA ESSAALHAHV NADYSQPSTS SSLLARGHPP RACNFTPSLS TEELTPDLTS
LDSVADSGRG SWTSCSSNSH ESFQSYPTSS CRPWDHGIYG HPLSLPHTHL GSFKHPARPV
AEAEAARPAW AGEDPRQPSR DSGSDRSAPQ SWASSSSISD NYEGNYGTVK RRIPEHPSSP
ANEEGAQSTD SAYKTVTSST EKGLIVYCVT SPTKDERYRA PPPTPPGYQG LALGDLGLSE
GVVGGPGGLV PRPPHLKPPD YSVAVQRSRL LQSPGAADGL AEARRAQLQH QDSRTSISKQ
HLHRPPSVCC PSQDLADSED DEQVSAV
//
