ID H2LRM3_ORYLA Unreviewed; 669 AA.
AC H2LRM3;
DT 21-MAR-2012, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 2.
DT 27-MAR-2024, entry version 65.
DE RecName: Full=Choline O-acetyltransferase {ECO:0000256|ARBA:ARBA00040495};
DE EC=2.3.1.6 {ECO:0000256|ARBA:ARBA00039091};
GN Name=chata {ECO:0000313|Ensembl:ENSORLP00000008722.2};
OS Oryzias latipes (Japanese rice fish) (Japanese killifish).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Atherinomorphae; Beloniformes; Adrianichthyidae; Oryziinae;
OC Oryzias.
OX NCBI_TaxID=8090 {ECO:0000313|Ensembl:ENSORLP00000008722.2, ECO:0000313|Proteomes:UP000001038};
RN [1] {ECO:0000313|Ensembl:ENSORLP00000008722.2, ECO:0000313|Proteomes:UP000001038}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hd-rR {ECO:0000313|Ensembl:ENSORLP00000008722.2,
RC ECO:0000313|Proteomes:UP000001038};
RX PubMed=17554307; DOI=10.1038/nature05846;
RA Kasahara M., Naruse K., Sasaki S., Nakatani Y., Qu W., Ahsan B., Yamada T.,
RA Nagayasu Y., Doi K., Kasai Y., Jindo T., Kobayashi D., Shimada A.,
RA Toyoda A., Kuroki Y., Fujiyama A., Sasaki T., Shimizu A., Asakawa S.,
RA Shimizu N., Hashimoto S., Yang J., Lee Y., Matsushima K., Sugano S.,
RA Sakaizumi M., Narita T., Ohishi K., Haga S., Ohta F., Nomoto H., Nogata K.,
RA Morishita T., Endo T., Shin-I T., Takeda H., Morishita S., Kohara Y.;
RT "The medaka draft genome and insights into vertebrate genome evolution.";
RL Nature 447:714-719(2007).
RN [2] {ECO:0000313|Ensembl:ENSORLP00000008722.2}
RP IDENTIFICATION.
RC STRAIN=Hd-rR {ECO:0000313|Ensembl:ENSORLP00000008722.2};
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh)
CC from acetyl CoA and choline at cholinergic synapses.
CC {ECO:0000256|ARBA:ARBA00037088}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + choline = acetylcholine + CoA;
CC Xref=Rhea:RHEA:18821, ChEBI:CHEBI:15354, ChEBI:CHEBI:15355,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.6;
CC Evidence={ECO:0000256|ARBA:ARBA00036720};
CC -!- SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.
CC {ECO:0000256|ARBA:ARBA00005232, ECO:0000256|RuleBase:RU003801}.
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DR AlphaFoldDB; H2LRM3; -.
DR Ensembl; ENSORLT00000008723.2; ENSORLP00000008722.2; ENSORLG00000006943.2.
DR eggNOG; KOG3717; Eukaryota.
DR GeneTree; ENSGT01060000248556; -.
DR HOGENOM; CLU_013513_3_1_1; -.
DR TreeFam; TF313836; -.
DR Proteomes; UP000001038; Chromosome 15.
DR Bgee; ENSORLG00000006943; Expressed in bone element and 6 other cell types or tissues.
DR GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 3.30.559.70; Choline/Carnitine o-acyltransferase, domain 2; 1.
DR InterPro; IPR000542; Carn_acyl_trans.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR039551; Cho/carn_acyl_trans.
DR InterPro; IPR042231; Cho/carn_acyl_trans_2.
DR PANTHER; PTHR22589; CARNITINE O-ACYLTRANSFERASE; 1.
DR PANTHER; PTHR22589:SF14; CHOLINE O-ACETYLTRANSFERASE; 1.
DR Pfam; PF00755; Carn_acyltransf; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 2.
DR PROSITE; PS00439; ACYLTRANSF_C_1; 1.
DR PROSITE; PS00440; ACYLTRANSF_C_2; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000256|RuleBase:RU003801};
KW Neurotransmitter biosynthesis {ECO:0000256|ARBA:ARBA00022979};
KW Reference proteome {ECO:0000313|Proteomes:UP000001038};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU003801}.
FT DOMAIN 18..593
FT /note="Choline/carnitine acyltransferase"
FT /evidence="ECO:0000259|Pfam:PF00755"
FT REGION 610..669
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 623..661
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 327
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR600542-1"
SQ SEQUENCE 669 AA; 75290 MW; 70F74A1145B30D9E CRC64;
SKQTEKAPAS LPIGLPKLPL PDLKDTLDMY LRCVKHLLTE EQFNKTQKIV KQFEAPGGVG
ELLQSKLVEK REKTANWVFD YWLNDMYLNN RVALPVNSSP VMVFPQQNFR APIDSLKFAA
HLISGVLEYK ALLDCSHDLP VDCARGQLAG TPLCMEQYHR LFTSHRLPGL EKDTLVALES
SMMPDPEHMI VACKNQFFVL NVVINFCRLN ERDLLIQLEK IKKMAESGED RLPPVGLLTS
DGRTEWAEAR SILMRESINR DSLDMIERSM CLVCLDEASG PELSDTTRAM LMLHGGGAAK
NGGNRWYDKP MQFVVGEDGC CGVVCEHSPF EGIVLVQCTE YLLKYMIGSP SKLVRAASVS
KLPAPRKLHW KCSPEIQKRI SSSADKLQRL VKNLDMNVHK FCDYGKEFIK KQKMSPDAFI
QVALQLAYYR CHGRMVSTYE SASTRRFQKG RVDNIRSATP EALAFIRAMT DGKLNSKKMR
MLREAITAQT NYTVLAISGM AIDNHLLGLR EIAQEMKMEK PEIFKDAAYL ISNHFLLSTS
QVPTTVEMFC CYGPVVQNGY GVCYNPQSDH IIFSVSSFHE SPETCSAEFV KHLLQGLQDM
MDLCNECSTD PNTAERRKGP TLEPHSQASQ LEKTLQRESD PTKGKSPAQT NVQIQSQSSA
KVKKNNEKK
//
