ID H2RWD2_TAKRU Unreviewed; 1135 AA.
AC H2RWD2;
DT 21-MAR-2012, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 2.
DT 27-MAR-2024, entry version 50.
DE SubName: Full=Solute carrier family 12 member 2 {ECO:0000313|Ensembl:ENSTRUP00000004452.2};
GN Name=slc12a2 {ECO:0000313|Ensembl:ENSTRUP00000004452.2};
OS Takifugu rubripes (Japanese pufferfish) (Fugu rubripes).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Tetraodontiformes; Tetradontoidea; Tetraodontidae; Takifugu.
OX NCBI_TaxID=31033 {ECO:0000313|Ensembl:ENSTRUP00000004452.2, ECO:0000313|Proteomes:UP000005226};
RN [1] {ECO:0000313|Ensembl:ENSTRUP00000004452.2, ECO:0000313|Proteomes:UP000005226}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21551351;
RA Kai W., Kikuchi K., Tohari S., Chew A.K., Tay A., Fujiwara A., Hosoya S.,
RA Suetake H., Naruse K., Brenner S., Suzuki Y., Venkatesh B.;
RT "Integration of the genetic map and genome assembly of fugu facilitates
RT insights into distinct features of genome evolution in teleosts and
RT mammals.";
RL Genome Biol. Evol. 3:424-442(2011).
RN [2] {ECO:0000313|Ensembl:ENSTRUP00000004452.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 chloride(out) + K(+)(out) + Na(+)(out) = 2 chloride(in) +
CC K(+)(in) + Na(+)(in); Xref=Rhea:RHEA:72395, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:29101, ChEBI:CHEBI:29103;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:72396;
CC Evidence={ECO:0000256|ARBA:ARBA00034631};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the SLC12A transporter family.
CC {ECO:0000256|ARBA:ARBA00010593}.
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DR RefSeq; XP_011602692.1; XM_011604390.1.
DR AlphaFoldDB; H2RWD2; -.
DR Ensembl; ENSTRUT00000004476.3; ENSTRUP00000004452.2; ENSTRUG00000001927.3.
DR GeneID; 101074482; -.
DR CTD; 6558; -.
DR GeneTree; ENSGT00940000155742; -.
DR OrthoDB; 5490251at2759; -.
DR Proteomes; UP000005226; Chromosome 6.
DR GO; GO:0098590; C:plasma membrane region; IEA:UniProt.
DR GO; GO:0015377; F:chloride:monoatomic cation symporter activity; IEA:InterPro.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1740.10; Amino acid/polyamine transporter I; 1.
DR InterPro; IPR004841; AA-permease/SLC12A_dom.
DR InterPro; IPR013612; AA_permease_N.
DR InterPro; IPR002444; NKCC1.
DR InterPro; IPR018491; SLC12_C.
DR InterPro; IPR002443; SLC12A1/SLC12A2.
DR InterPro; IPR004842; SLC12A_fam.
DR NCBIfam; TIGR00930; 2a30; 1.
DR PANTHER; PTHR11827:SF58; SOLUTE CARRIER FAMILY 12 MEMBER 2; 1.
DR PANTHER; PTHR11827; SOLUTE CARRIER FAMILY 12, CATION COTRANSPORTERS; 1.
DR Pfam; PF00324; AA_permease; 1.
DR Pfam; PF08403; AA_permease_N; 1.
DR Pfam; PF03522; SLC12; 1.
DR PRINTS; PR01207; NAKCLTRNSPRT.
DR PRINTS; PR01208; NAKCLTRSPRT1.
PE 3: Inferred from homology;
KW Ion transport {ECO:0000256|ARBA:ARBA00023201};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000005226};
KW Sodium {ECO:0000256|ARBA:ARBA00023053};
KW Sodium transport {ECO:0000256|ARBA:ARBA00023201};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00023201}.
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 248..274
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 295..316
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 342..361
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 368..388
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 416..435
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 447..466
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 532..555
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 588..608
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 614..632
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 644..667
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 133..184
FT /note="Amino acid permease N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08403"
FT DOMAIN 221..725
FT /note="Amino acid permease/ SLC12A"
FT /evidence="ECO:0000259|Pfam:PF00324"
FT DOMAIN 734..1135
FT /note="SLC12A transporter C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03522"
FT REGION 1..111
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 890..922
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 55..71
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 899..922
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1135 AA; 124087 MW; 1E0C3CAF7DEB402D CRC64;
MSSPSSAPSA PPEDATAEND FLTADTGLRP LGPTPSQSRF QVDLVAESAG AAEDKSPSSD
VSTTVPSSDK SSAPDGEEAK GRFRVVNFAD PRGAGSAASP EAPPAEGIQN GDTVMSETSI
HSSTGGQHHY HYDTHTNTYY LRTFGHNTID AVPNIDFYRQ TAAPLGEKLI RPTLSELHDE
LDKEPFEDGF ANGEELTPAE EAAAKESAES KGVVKFGWIK GVLVRCMLNI WGVMLFIRMS
WIVGQAGIAF SCLIVLMATV VTTITGLSTS AIATNGFVRG GGAYYLISRS LGPEFGGSIG
LIFAFANAVA VAMYVVGFAE TVVELLAGAD AIMTDNINDV RIIGTITVIL LLGISVAGME
WEAKAQIFLL IVLITAIINY FIGTFISVES KKAFGFFGYD GSLLWENMGP DFRGETFFSV
FAIFFPAATG ILAGANISGD LADPQLAIPR GTLLAILITG IVYLGVAVST GSCIVREATG
NINSTVSSQF VANCTDAACK FGFDFSSCKD PAADCKYGLL HDFQVMSMVS GFGPIITAGI
FSATLSSALA SLVSAPKVFQ ALCKDNIYPG LGIFAKGYGK NNEPLRGYIL TFGIALAFIL
IAELNIIAPI ISNFFLASYA LINFSVFHAS LAKSPGWRPS FKYYNMWVSL AGAILCCVVM
FVINWWAALL TNVIVLGLYI YVSYKKPDVN WGSSTQALTY HQALTHTLRL SGVEDHIKNF
RPQCLVMTGY PNSRPALLDL VHSFTKNVGL MICGHVRMGY RRPNYKELAT DQARYQRWLL
KHECKAFYTP VFAEDLKQGT QYLLQATGLG RLKPNTLVLG FKNDWRDGDM MNVETYISMI
HDAFDFQFGA VILRLKEGLD VSHIQGQDDL LSSQEKSSGM KDVIVSIDTS KDSDADSSKP
SSKATSLQNS PAIQKDKKSP TVPLNVSDQR LLEASQQFQN KQGKGTVDVW WLFDDGGLTL
LIPYLLTNKK RWKDCKIRVF IGGKINRIDH DRRAMATLLS KFRIDFSDIT VLGDVNTKPK
MEHVSAFEEL IEPYRLKEDD LEPEAAERLK NSEPWRITDN ELELYRAKTN RQIRLNELLK
EHSSTANLIV MSLPLARKGA VSSALYMAWL EVLSRELPPI LLVRGNHQSV LTFYS
//
