ID H2SY47_TAKRU Unreviewed; 1199 AA.
AC H2SY47;
DT 21-MAR-2012, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 3.
DT 27-MAR-2024, entry version 65.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN Name=iars1 {ECO:0000313|Ensembl:ENSTRUP00000017335.3};
OS Takifugu rubripes (Japanese pufferfish) (Fugu rubripes).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Tetraodontiformes; Tetradontoidea; Tetraodontidae; Takifugu.
OX NCBI_TaxID=31033 {ECO:0000313|Ensembl:ENSTRUP00000017335.3, ECO:0000313|Proteomes:UP000005226};
RN [1] {ECO:0000313|Ensembl:ENSTRUP00000017335.3, ECO:0000313|Proteomes:UP000005226}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21551351;
RA Kai W., Kikuchi K., Tohari S., Chew A.K., Tay A., Fujiwara A., Hosoya S.,
RA Suetake H., Naruse K., Brenner S., Suzuki Y., Venkatesh B.;
RT "Integration of the genetic map and genome assembly of fugu facilitates
RT insights into distinct features of genome evolution in teleosts and
RT mammals.";
RL Genome Biol. Evol. 3:424-442(2011).
RN [2] {ECO:0000313|Ensembl:ENSTRUP00000017335.3}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR AlphaFoldDB; H2SY47; -.
DR Ensembl; ENSTRUT00000017409.3; ENSTRUP00000017335.3; ENSTRUG00000007056.3.
DR eggNOG; KOG0434; Eukaryota.
DR GeneTree; ENSGT00550000074921; -.
DR HOGENOM; CLU_001493_1_0_1; -.
DR Proteomes; UP000005226; Chromosome 19.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 3.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000005226}.
FT DOMAIN 18..527
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 529..570
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 625..781
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1199 AA; 137111 MW; 60D418F98D8EF8F6 CRC64;
MVEPVSESIH FPSEEEKIMQ FWKEKDCFQE CLKQSKNRPK YTFYDGPPFA TGLPHYGHIL
AGTIKDVVTR FAHQSGFHVE RRFGWDCHGL PVEYEIDKTL GIKGPEDVAK MGIAEYNNQC
RGIVMRYSNE WESSVKRMGR WIDFKNDYKT LYPWFMETVW WVFKQLYDKG LVYRGVKVMP
FSTACNTPLS NFESHQNYKD VQDPSVIVNF PLVGNEEVAL IAWTTTPWTL PSNLALCVNP
EMLYVKVKGD KNNKLYSFNL QLCRAFQVVT DNYVKEEEGT GVVHQAPYFG ADDYRVCTEF
NIIQRDQAPI CPVDASGCFT SEVTDFAGQY VKDADKNIIK WLKENGRLVN ASSFKHSYPF
CWRSDTPLIY KAVPSWFVRV EHMVEKLLDN NGKCYWVPEF VREKRFGNWL RDARDWAISR
NRYWGTPIPL WVSDDFEEVV CIGSVADLEE LTGVKVTDLH RESIDSLTIP SRCGKGVLRR
VSEVFDCWFE SGSMPYAQVH YPFENRKEFE DTFPADFIAE GIDQTRGCDG QKMSKRKKNY
PDPGLIVQNY GADALRLYLI NSPVVRAENL RFKEEGVRDV LKDVFLPWYN AYRFLVQNVQ
RLQKDEDIQF LYNENTAKQS DNIMDKWIQS FTQSLIQFFK EEMDAYRLYT VVPRLVRFVD
MLTNWYVRTN RRRLKGESGT EDCLWALETL FSVLFSMCRL MAPFTPFITE IMYQNLQHLI
DPVSVEEKDA TSIHYLMLPQ VRESLIDKRI ENAVSQMQSV IELGRVIRDR KTMPIKYPLK
EVVVIHQDPE ALKDIQSLQK YILEELNVRQ LTLSTDKDKY GIRLRAEPDH MVLGKRLKGA
FKAVTASIKE LTSEQLEVFQ KTGCIVVDGH ELHEEDLRLM YAFNQASGSA AQYEAHSDSQ
VLVLLDVSPD QSMVDEGVAR EVINRIQKLR KKAHLVPTDE ISVYYRCQPE GDYLNSVVQA
HTDFIMGTTK APLLPFPVPK RASVIIAEKT QLKNSDLELT IVKGSSAQAP LNGPACAYVN
LRLHVNNKET EGVVLLENPR GDNRLDLHKL RKISSSIFAT NNTQLKFFSG GTELTNKMDL
LALNGKTLTV TQGSSSPGPA PTTSSLLCPY VNLLLCNAQP SECLTGVAAT LLLANPVGQN
GLDFSGLLSE ASKVFGLRGR RLKLYLDQDL SQGKMNGEEY QTLDSKTLFV RVLPTTADA
//
