ID H2TJE6_TAKRU Unreviewed; 881 AA.
AC H2TJE6;
DT 21-MAR-2012, integrated into UniProtKB/TrEMBL.
DT 17-JUN-2020, sequence version 3.
DT 27-MAR-2024, entry version 69.
DE RecName: Full=Alpha-actinin-1 {ECO:0000256|ARBA:ARBA00018384};
DE AltName: Full=Alpha-actinin cytoskeletal isoform {ECO:0000256|ARBA:ARBA00041477};
DE AltName: Full=F-actin cross-linking protein {ECO:0000256|ARBA:ARBA00043249};
DE AltName: Full=Non-muscle alpha-actinin-1 {ECO:0000256|ARBA:ARBA00042871};
GN Name=LOC101075286 {ECO:0000313|Ensembl:ENSTRUP00000024800.3};
OS Takifugu rubripes (Japanese pufferfish) (Fugu rubripes).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Eupercaria; Tetraodontiformes; Tetradontoidea; Tetraodontidae; Takifugu.
OX NCBI_TaxID=31033 {ECO:0000313|Ensembl:ENSTRUP00000024800.3, ECO:0000313|Proteomes:UP000005226};
RN [1] {ECO:0000313|Ensembl:ENSTRUP00000024800.3, ECO:0000313|Proteomes:UP000005226}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=21551351;
RA Kai W., Kikuchi K., Tohari S., Chew A.K., Tay A., Fujiwara A., Hosoya S.,
RA Suetake H., Naruse K., Brenner S., Suzuki Y., Venkatesh B.;
RT "Integration of the genetic map and genome assembly of fugu facilitates
RT insights into distinct features of genome evolution in teleosts and
RT mammals.";
RL Genome Biol. Evol. 3:424-442(2011).
RN [2] {ECO:0000313|Ensembl:ENSTRUP00000024800.3}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cell junction {ECO:0000256|ARBA:ARBA00004282}.
CC Cell membrane {ECO:0000256|ARBA:ARBA00004236}. Cell projection, ruffle
CC {ECO:0000256|ARBA:ARBA00004466}. Cytoplasm, myofibril, sarcomere, Z
CC line {ECO:0000256|ARBA:ARBA00004216}. Membrane
CC {ECO:0000256|ARBA:ARBA00004370}.
CC -!- SIMILARITY: Belongs to the alpha-actinin family.
CC {ECO:0000256|ARBA:ARBA00010255}.
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DR AlphaFoldDB; H2TJE6; -.
DR STRING; 31033.ENSTRUP00000067973; -.
DR Ensembl; ENSTRUT00000024901.3; ENSTRUP00000024800.3; ENSTRUG00000011100.3.
DR GeneTree; ENSGT00940000155548; -.
DR Proteomes; UP000005226; Chromosome 2.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0001726; C:ruffle; IEA:UniProtKB-SubCell.
DR GO; GO:0030018; C:Z disc; IEA:UniProtKB-SubCell.
DR GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR CDD; cd21216; CH_ACTN_rpt2; 1.
DR CDD; cd00051; EFh; 1.
DR CDD; cd00176; SPEC; 1.
DR Gene3D; 1.20.58.60; -; 4.
DR Gene3D; 1.10.418.10; Calponin-like domain; 2.
DR Gene3D; 1.10.238.10; EF-hand; 3.
DR InterPro; IPR001589; Actinin_actin-bd_CS.
DR InterPro; IPR001715; CH_dom.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR014837; EF-hand_Ca_insen.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR018159; Spectrin/alpha-actinin.
DR InterPro; IPR002017; Spectrin_repeat.
DR PANTHER; PTHR11915:SF434; ALPHA-ACTININ-1; 1.
DR PANTHER; PTHR11915; SPECTRIN/FILAMIN RELATED CYTOSKELETAL PROTEIN; 1.
DR Pfam; PF00307; CH; 2.
DR Pfam; PF13405; EF-hand_6; 1.
DR Pfam; PF13833; EF-hand_8; 1.
DR Pfam; PF08726; EFhand_Ca_insen; 1.
DR Pfam; PF00435; Spectrin; 4.
DR SMART; SM00033; CH; 2.
DR SMART; SM00054; EFh; 2.
DR SMART; SM01184; efhand_Ca_insen; 1.
DR SMART; SM00150; SPEC; 3.
DR SUPFAM; SSF47576; Calponin-homology domain, CH-domain; 1.
DR SUPFAM; SSF47473; EF-hand; 2.
DR SUPFAM; SSF46966; Spectrin repeat; 4.
DR PROSITE; PS00020; ACTININ_2; 1.
DR PROSITE; PS50021; CH; 2.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 2.
PE 3: Inferred from homology;
KW Actin-binding {ECO:0000256|ARBA:ARBA00023203};
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Cell junction {ECO:0000256|ARBA:ARBA00022949};
KW Cell projection {ECO:0000256|ARBA:ARBA00023273};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000005226};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT DOMAIN 1..102
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000259|PROSITE:PS50021"
FT DOMAIN 111..217
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000259|PROSITE:PS50021"
FT DOMAIN 713..748
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 776..811
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT REGION 13..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 232..259
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 397..431
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 881 AA; 100885 MW; DE7560A48A8F8248 CRC64;
PEHLSNVYSL VQLPPEESGH TDREHRGGFP GWPQTHAAAG GERLAKPERG KMRVHKISNV
NKALDFIASK GVKLVSIGAE EIVDGNAKMT LGMIWTIILR FAIQDISVEE TSAKEGLLLW
CQRKTAPYKN VNIQNFHISW KDGLGFCALI HRHRPELIDY GKLRKDDPMT NLNTAFDVAE
KYLDIPKMLD AEDIVGTARP DEKAIMTYVS SFYHAFSGAQ KAETAANRIC KVLAVNQENE
QLMEDYERLA SDLLEWIRRT IPWLENRMPE NTMQAMQQKL EDFRDYRRLH KPPKVQEKCQ
LEINFNTLQT KLRLSNRPAF MPSEGKMVSD IINAWGNLEG AEKGYEEWLL NEIRRLERLD
HLAEKFRQKA TIHEAWTEGQ EEVLQKHDYE TASLSEIKAL LKKHEAFESD LAAHQDRVEQ
IAAIAQELNE LDYYDSPSVN ARCQRICDQW DTLGALTQKR GEALQRTGKL LETIDQLYLE
FAKRAAPFNN WMESAMEDLQ DTFIVHTIEE IQGLSTAHEQ FKATLPEADK ERQAILGIHN
EIAKIVQTYH VNMAGTNPYT TITPQEVNTK WDKVKQLVPQ RDQALIEEHA RQQNNERLRR
QFANQANVIG PWIQTKMEEI GRISIEMHGT LEDQLQHLRQ YEKSIVNYKP KIDQLEGDHQ
LIQEALIFDN KHTKYTMEHI RVGWEQLLTT IARTINEIEN QILTRDAKGI SQEQLNEFRA
SFNHFDRDHS GTLGAEEFKA CLISLGFDIA NDAQKRSGIM DAEDFKTCLI SMGYNLGESE
FSRIMSVVDP NRMGLVTFQA FIDFMSRETA DTDTADQVIA SFKVLAGDKN YILADELRRE
LPPDQAEYCI ARMAPYTGPD GVPGALDYMS FSTALYGESD L
//
