ID H3ALX5_LATCH Unreviewed; 1452 AA.
AC H3ALX5;
DT 18-APR-2012, integrated into UniProtKB/TrEMBL.
DT 18-APR-2012, sequence version 1.
DT 27-MAR-2024, entry version 79.
DE RecName: Full=Rap guanine nucleotide exchange factor 2 {ECO:0000256|ARBA:ARBA00016709};
DE AltName: Full=Cyclic nucleotide ras GEF {ECO:0000256|ARBA:ARBA00031545};
DE AltName: Full=Neural RAP guanine nucleotide exchange protein {ECO:0000256|ARBA:ARBA00032021};
DE AltName: Full=PDZ domain-containing guanine nucleotide exchange factor 1 {ECO:0000256|ARBA:ARBA00030673};
DE AltName: Full=RA-GEF-1 {ECO:0000256|ARBA:ARBA00029925};
DE AltName: Full=Ras/Rap1-associating GEF-1 {ECO:0000256|ARBA:ARBA00031980};
GN Name=RAPGEF2 {ECO:0000313|Ensembl:ENSLACP00000010646.1};
OS Latimeria chalumnae (Coelacanth).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Coelacanthiformes; Coelacanthidae; Latimeria.
OX NCBI_TaxID=7897 {ECO:0000313|Ensembl:ENSLACP00000010646.1, ECO:0000313|Proteomes:UP000008672};
RN [1] {ECO:0000313|Proteomes:UP000008672}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Wild caught {ECO:0000313|Proteomes:UP000008672};
RA Di Palma F., Alfoldi J., Johnson J., Berlin A., Gnerre S., Jaffe D.,
RA MacCallum I., Young S., Walker B.J., Lander E., Lindblad-Toh K.;
RT "The draft genome of Latimeria chalumnae.";
RL Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSLACP00000010646.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Cell junction {ECO:0000256|ARBA:ARBA00004282}.
CC Cell membrane {ECO:0000256|ARBA:ARBA00004236}. Cytoplasm, perinuclear
CC region {ECO:0000256|ARBA:ARBA00004556}. Endosome
CC {ECO:0000256|ARBA:ARBA00004177}. Late endosome
CC {ECO:0000256|ARBA:ARBA00004603}.
CC -!- SIMILARITY: Belongs to the RAPGEF2 family.
CC {ECO:0000256|ARBA:ARBA00010829}.
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DR EMBL; AFYH01113660; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AFYH01113661; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AFYH01113662; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AFYH01113663; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR Ensembl; ENSLACT00000010725.1; ENSLACP00000010646.1; ENSLACG00000009378.2.
DR GeneTree; ENSGT00940000156418; -.
DR Proteomes; UP000008672; Unassembled WGS sequence.
DR Bgee; ENSLACG00000009378; Expressed in post-anal tail muscle and 6 other cell types or tissues.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-SubCell.
DR GO; GO:0005770; C:late endosome; IEA:UniProtKB-SubCell.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
DR CDD; cd00038; CAP_ED; 1.
DR CDD; cd00992; PDZ_signaling; 1.
DR CDD; cd01785; RA_PDZ-GEF1; 1.
DR CDD; cd00155; RasGEF; 1.
DR CDD; cd06224; REM; 1.
DR Gene3D; 2.30.42.10; -; 1.
DR Gene3D; 2.60.120.10; Jelly Rolls; 1.
DR Gene3D; 1.10.840.10; Ras guanine-nucleotide exchange factors catalytic domain; 1.
DR Gene3D; 1.20.870.10; Son of sevenless (SoS) protein Chain: S domain 1; 1.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR018490; cNMP-bd_dom_sf.
DR InterPro; IPR001478; PDZ.
DR InterPro; IPR036034; PDZ_sf.
DR InterPro; IPR000159; RA_dom.
DR InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR InterPro; IPR023578; Ras_GEF_dom_sf.
DR InterPro; IPR001895; RASGEF_cat_dom.
DR InterPro; IPR036964; RASGEF_cat_dom_sf.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR PANTHER; PTHR45161; CYTOSKELETON-ASSOCIATED PROTEIN 4; 1.
DR PANTHER; PTHR45161:SF2; RAP GUANINE NUCLEOTIDE EXCHANGE FACTOR 2; 1.
DR Pfam; PF00595; PDZ; 1.
DR Pfam; PF00788; RA; 1.
DR Pfam; PF00617; RasGEF; 1.
DR Pfam; PF00618; RasGEF_N; 1.
DR SMART; SM00100; cNMP; 1.
DR SMART; SM00228; PDZ; 1.
DR SMART; SM00314; RA; 1.
DR SMART; SM00147; RasGEF; 1.
DR SMART; SM00229; RasGEFN; 1.
DR SUPFAM; SSF51206; cAMP-binding domain-like; 1.
DR SUPFAM; SSF50156; PDZ domain-like; 1.
DR SUPFAM; SSF48366; Ras GEF; 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 1.
DR PROSITE; PS50042; CNMP_BINDING_3; 1.
DR PROSITE; PS50106; PDZ; 1.
DR PROSITE; PS50200; RA; 1.
DR PROSITE; PS50009; RASGEF_CAT; 1.
DR PROSITE; PS50212; RASGEF_NTER; 1.
PE 3: Inferred from homology;
KW Cell junction {ECO:0000256|ARBA:ARBA00022949};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Differentiation {ECO:0000256|ARBA:ARBA00022782};
KW Endosome {ECO:0000256|ARBA:ARBA00022753};
KW Guanine-nucleotide releasing factor {ECO:0000256|ARBA:ARBA00022658,
KW ECO:0000256|PROSITE-ProRule:PRU00168};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Neurogenesis {ECO:0000256|ARBA:ARBA00022902};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000008672};
KW Ubl conjugation {ECO:0000256|ARBA:ARBA00022843}.
FT DOMAIN 63..163
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 195..309
FT /note="N-terminal Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50212"
FT DOMAIN 313..383
FT /note="PDZ"
FT /evidence="ECO:0000259|PROSITE:PS50106"
FT DOMAIN 539..624
FT /note="Ras-associating"
FT /evidence="ECO:0000259|PROSITE:PS50200"
FT DOMAIN 649..876
FT /note="Ras-GEF"
FT /evidence="ECO:0000259|PROSITE:PS50009"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 935..986
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 995..1014
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1029..1083
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1177..1211
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1275..1358
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1401..1452
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..24
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 954..986
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1039..1083
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1293..1307
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1310..1339
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1452 AA; 162468 MW; 5CC9F693B59976BC CRC64;
MDLSGLPETA VDSEDDDDEE DIERASDPLM SRDVVRDCLE KDPIDRTDDD IEQLLEFMHQ
LPAFANMTMS VRKELCAVMV FAVVERAGTI VLNDGEELDS WSVILNGSVE VTYPDGRTEI
LCMGNSFGVS PTMEKEYMKG VMKTKVDDCQ FVCIAQQDYC RILNQVEKNM QKVEEEGEIV
MVKEHRELDR TGTRKGHIVI KGTPERLTMH LVEEHSVVDP TFIEDFLLTY RTFLSSPMEV
GKKLLEWFND PSLRDKVTRV VLLWVNNHFN DFEGDTAMTR FLEEFENSLE REKMMGHLRL
LNIACAAKAK RRLITLTKPS REAPLPFILL GGSEKGLGIF VDSVESGSKA AETGLKRGDR
ILEVNGQNFE NVQLSKAMEI LKNNTHLSIT VKTNLFEFKQ LLTRLAEEKR NGAPHLPKIG
DIKKASRYSI PDLAVDVEQV MGLEKVNKKS KANTVGGKNK LKKIFDKTRI SILPQKPYND
IGIGQSQDDS IVGLRQSKHI PPTLPVSGNL SSSNPDLLQS HHRILDFNNS PAAGFYNLPD
QVLRVFKADQ QSRYIMISKD TTAKEVVHQA LREFALTGPE NYSLCEVSVT PEGVIKQKRL
PDQLSKLADK IQLSGRYYLK NNMETETLCS DEDAQELLKE SQISLLQLST IEVATQLSMR
NFELFRNIEA TEYIDDLFKL KTKVGSSNLK KFEEVINQET FWVASEILKE LNQIKRMKII
KHFIKIALHC RECKNFNSMF AIISGLNLAP VARLRSTWEK LNSKYEKLFQ DLQDLFDPSR
NMAKYRNVLN SQNLQPPIIP LFPVIKKDLT FLHEGNDSKV EGLVNFEKLR MIAKEIRHVG
RMASVNMDPA LMFRTRKKKW RSLGSLSQGS TNAALDVVQT GGHKKRVRRS SFLNAKKLYE
DAQMARKVKQ YLANLVLEMD EDSLQVLSLV CEPSTNTLPK NAGDRRSGKC DTSPVTPRAG
TQQPKTQQPQ PQQQQPQQQQ QKGNQALQVP PVSLYPSRKK VPVKDLPPFG TSSPQALRKI
LSLSEEANFD RHKKQAEDTI SNASSQLSSP PTSPQTSPRK GGSQLRSHGS RHSDLTGSSS
SLGSYTLAPS GTVDNFSDSG HSEISSRSSI VSNSSFDCVP MHDEKRQRHS VSIVEANLGV
GRIDRRAVMD PDQYSFSSYS PVPENRSLYA GATVLSSPST EELTQDQGDR ASLDAADSGR
GSWTSCSSGS HDNIQTIQHQ RSWETLAFGH THFDSAAEGA ALWASGNHMD QLMFSELANK
CSRYSQAREN LDQAQSRGSW ASSPGYWGEE TEGDTGTIKR RGGKDVAAEA ETSSITSLTS
EESKNIRPSH ITVSSSTAKG LIARKEGRYR EPPPTPPGYI GIPIADFPEV SSFQTRKPPD
YNVALQRSRF VARPCEPLGA SATQQLHGHP AARTVNKPQW HRPNESDPRL ALYQSQGFSA
EEEDEDEQVS AV
//
