ID H3BHM1_LATCH Unreviewed; 203 AA.
AC H3BHM1;
DT 18-APR-2012, integrated into UniProtKB/TrEMBL.
DT 18-APR-2012, sequence version 1.
DT 27-MAR-2024, entry version 53.
DE RecName: Full=tRNA pseudouridine synthase {ECO:0000256|RuleBase:RU003792};
DE EC=5.4.99.12 {ECO:0000256|RuleBase:RU003792};
GN Name=PUSL1 {ECO:0000313|Ensembl:ENSLACP00000021392.1};
OS Latimeria chalumnae (Coelacanth).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Coelacanthiformes; Coelacanthidae; Latimeria.
OX NCBI_TaxID=7897 {ECO:0000313|Ensembl:ENSLACP00000021392.1, ECO:0000313|Proteomes:UP000008672};
RN [1] {ECO:0000313|Proteomes:UP000008672}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Wild caught {ECO:0000313|Proteomes:UP000008672};
RA Di Palma F., Alfoldi J., Johnson J., Berlin A., Gnerre S., Jaffe D.,
RA MacCallum I., Young S., Walker B.J., Lander E., Lindblad-Toh K.;
RT "The draft genome of Latimeria chalumnae.";
RL Submitted (AUG-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSLACP00000021392.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA;
CC Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-COMP:10087,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12;
CC Evidence={ECO:0000256|RuleBase:RU003792};
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000256|ARBA:ARBA00009375, ECO:0000256|RuleBase:RU003792}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AFYH01000412; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AFYH01000413; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AFYH01000414; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AFYH01000415; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AFYH01000416; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AFYH01000417; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AFYH01000418; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AFYH01000419; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AFYH01000420; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AFYH01000421; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; H3BHM1; -.
DR STRING; 7897.ENSLACP00000021392; -.
DR Ensembl; ENSLACT00000021533.1; ENSLACP00000021392.1; ENSLACG00000018795.1.
DR eggNOG; KOG4393; Eukaryota.
DR GeneTree; ENSGT00950000183160; -.
DR HOGENOM; CLU_014673_3_1_1; -.
DR InParanoid; H3BHM1; -.
DR OMA; RSICATT; -.
DR TreeFam; TF105127; -.
DR Proteomes; UP000008672; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR11142:SF0; TRNA PSEUDOURIDINE SYNTHASE-LIKE 1; 1.
DR Pfam; PF01416; PseudoU_synth_1; 2.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|RuleBase:RU003792};
KW Reference proteome {ECO:0000313|Proteomes:UP000008672};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694,
KW ECO:0000256|RuleBase:RU003792}.
FT DOMAIN 10..81
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
FT DOMAIN 126..193
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
SQ SEQUENCE 203 AA; 23357 MW; 8AF7064078D752D5 CRC64;
YLEAAARKLR PVNEINFSIS SRTDSGVHAL CNSAHFDVQR RDGMPPFSED LLVDALNYHL
KPEPISVLKA YRMPDNFHAR YRAVSRTYMY RLVTGICHHS QLPVFERNLC WPICESPLNI
GAMQEAAQLF LGTHDFSAFR SISPDNPLKS PIKTLLQADV RSSSGCFSYL HRHRDLQFWE
LIFKSKSFLY KQVCNHFDSC LTT
//
